Ammonium Nitrate Lab Report

In this experiment we are testing the effect of fertilizer on the speed of plant growth. We prepared a 4 quad cell, 1 control group and 3 experimental groups. So, we had one with no fertilizer, one with three seeds of fertilizer, one with six seeds of fertilizer, and lastly, one with nine seeds of fertilizer. The plants that we grew were called Wisconsin Fast Plants, members of the crucifer family. These plants are small and easy to grow, but for optimal growth they require continuous fertilizer, water, fluorescent light, and temperature between 18 degrees Celsius and 26 degrees Celsius 24 hours a day. Fertilizers are substances that are put into soils to increase the growth of the plant. There are two different types of fertilizers, synthetic

The experiment was begun by obtaining four 8 oz. Styrofoam cups and punching a hole through the bottom of them. This hole was for water entry or excess water drainage. Moistened soil was packed to the 1/2 full line in the cup along with 3 fertilizer pellets The cups were labeled the following: Rosette-H20, Rosette-GA, Wild-Type-H2O, and Wild-type- GA.(Handout 1) A small wooden applicator stick was obtained a moistened at the tip with water from the petri dish labeled ‘water.’ This was to be able to attract the seed to the applicator in order to place the seed from its original container into

Our data recorded shows that the germinating peas did consume more oxygen than the non-germinating or the glass beads alone and that the cooler temperature did slow down the consumption of oxygen in the germinating peas. In both water baths the atmospheric pressure seemed to increase causing our reading to raise in our glass beads and non-germinating peas. This direct relationship in reading leads us to believe that the oxygen consumption in the non-germinating peas was minimal if any at all.

The seeds soaked in the salt solution did not have any change in appearance while the seeds exposed to the vinegar solution generally appeared to become darker in color and shriveled. For the 10% fertilizer solution only one replication produced germination (6 out of the 10 seeds in the petri dish germinated). The seeds that did not germinate for the 10% fertilizer solution appeared darker in color. Seeds exposed to distilled water (control) had germination across every replication group and produced some fairly large sprouts. The 10% soap solution seeds also had germination in every replication group, but the size of the sprouts were not as large as the ones in the control group.

The results obtained are non-conclusive. More research is necessary in order to fully understand the effects of Nitrogen in the development of Fast Plants seeds and the soil. It is recommended that original is repeated. However, only one fertilizer should be added per quad, rather than mixing the fertilizer used for the control with the fertilizer being studied. For future studies it is also recommended to maintain a record of the pH of the soils before, during, and after the experiment in order to understand the impact of fertilizers on the

Throughout this experiment, we are researching the effect on the growth and survival of Wisconsin Fast Plants using fertilizer pellets to help with the growth of the plants. Wisconsin Fast Plants is a plant member of the crucifer family which is related to other plants (vegetables) such as cabbage, broccoli, turnips, etc. This plants are small and can grow very easily because they go through their cell cycle around 40 days. Wisconsin Fast Plants Fertilizers are different materials used that can provide plants with the nutrients it need to grow. (1) These plants are a good model system to study because they grew very quickly and didn’t need a lot of resources to grow making them the perfect plant to use for studies. (4) By using the fertilizers,

Strontium nitrate, the limiting reagent, was poured into the copper(II) sulphate solution, the excess reagent, rather than the other way around. This is a minor systemic error, because even if some of the strontium nitrate remained in its initial beaker, the beaker was thoroughly rinsed out multiple times. The error causes the final mass of the filter paper and precipitate to be slightly decreased, as not all of strontium nitrate reacts with the copper(II) sulfate. This can explain why the percent yield in 92.2%.

Experiment 1 (Assignment 3): Using sciccors, leaves from the Geranium plant were cut (Plant A was the bigger leaf and Plant B was the smaller leaf). Then begin to heat up the hot plate to boiling temperature of 100℃. Next one beaker was filled with ⅔ of water and another beaker was filled with ⅓ alcohol. Place the beaker of water onto the hot plate until boiling. To speed up the boiling process put boiling chips into the beaker. Then put Plant A (the leaf exposed to air) into the boiling water for 3-5 minutes. After time is up, using tongs, place Plant A directly into the alcohol solution for another 3-5 minutes. When time is up, take out Plant A and place it into a clean petri dish. Once the plant is properly placed, cover the leaf completely

The first error we made was not watering the grass seeds in the terrestrial chamber at a constant rate; this resulted in the grass not growing. We planned to water the grass seeds for 50 mL every week, but ended up only watering the seeds at random times with random amounts of water. Another error that we made was when we were testing for the dissolved oxygen in the aquatic chamber. We did not know that if we left the probe in for too long the dissolved oxygen will decrease. During week 2 of testing for the dissolved oxygen we left the probe in for too long and that changed our results dropping the amount collected down to 5.4, if we did not leave the probe in for too long the result might have came out to be around 8.0 to 10.0.

A. ) The glycolytic enzyme produces NADH+ and carbon dioxide from pyruvate. The allosteric control of the glycolytic enzyme is controlled by the amount of NADH produced. The NADH+ and carbon dioxide tricarboxylic acid cycle and become NADH+ and FAH2 The higher concentration of NADH, will lead to more electrons being contributed to the electron transport cycle, increasing the hydrogen ion gradient across the inner mitochondrial membrane, which would lead to an increased production of ATP.

METHODS/PROCEDURES: In the beginning of the experiment, pea seeds were used in order to perform the experiment. It was extremely important to acquire good, dry, and viable seeds so the process of germination could occur. A handful of these healthy seeds worked best in assisting the experiment. The seeds ability to germinate was a vital information needed to determine the outcome of the experiment.

Our theoretical yield of Silver is 0.370 g, and our actual yield is 0.351 g. To begin with, I was amazed of the process of making silver from a little piece of metal and silver nitrate solution even though it took 3 days to complete this lab. On the first day, Michael mostly did all the measurements and testing, while Mai and I was responsible for the calculations, including taking pictures and recording down the data. Firstly, our group balanced out the chemical equation, which is very important for the later steps of our procedure. The equation is 1Cu+2AgNO3Cu(NO3)2 +2Ag ( you can see how to balance it out in the procedure and we are using Copper II for this lab).

Hypothesis for experiment 1: The hypothesis for experiment 1 was that cell fraction pellet 2 would contain the most chloroplasts. Figure 2C on page 43 in the lab notebook shows that centrifugation of a plant cell resulting in 3 pellets will result in the second pellet containing the most chloroplasts because it is lighter and less dense when compared to nuclei and amyloplasts but more dense than mitochondria(Mcallister and Leicht 43). We created 2 pellets so it would make sense that the last pellet will contain the most chloroplasts. Question for experiment 2: Using the cell fraction that was identified in the previous experiment as containing the most chloroplasts, how much effect will diuron, an herbicide used in Iowa, have on the electron transport chain?(Mcallister and Leicht

Studies have shown that under varying light conditions, different bryophyte species result in different growth responses. For example, although most of the tested bryophyte exhibited greater growth responses when irradiance levels decreased, this was not the case for every bryophyte species. Unlike the other 5 bryophyte moss species that Rincón observed, L. bidentate did not exhibit the typical decrease in growth with the increased irradiance levels (Rincón, 1993). This indicates that the variation in different species can affect the growth of bryophyte moss, which relates to the amount of microbial diversity and their abundance. Likewise, in North Carolina, researchers specifically studied the chlorophyll a/b ratios in mosses. The presence of chlorophyll a and b is indicative of the amount of photosynthesis a plant undergoes. Photosynthesis is a very important process that allows plants to produce their own food, glucose sugar, for proper plant growth and development. A variety of moss species were studied and their chlorophyll a/b ratios were shown to be less than or equal to 2.1 (Martin, 1980). Vascular plants would have higher chlorophyll a/b ratios because they require greater light exposure for growth and development. As we have observed in this experiment, this is not the case for moss. The darker bottom samples showed greater microbial diversity and abundance. In the same way, mosses grow better in environments with less light, which explains why the 11 mosses studied by Martin have a lower chorophyll a/b ratio than a typical vascular plant (Martin, 1980). In addition, mosses that vary in this ratio can show different degrees of microbial diversity and abundance, whether they are sampled in the same or different light

Figure 2. Mean seedling biomass (g) for the control and each concentration of the nutrient complement, along with standard deviation error bars. Each experimental treatment had a sample size of 20 that