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Biotechnology: Forward And Reverse Genetic

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Forward and reverse genetics are ways to determine what genes or proteins are made by mutating or editing specific genes or proteins. In forward genetics, there is a specific phenotype and the goal is to figure out what genes cause that phenotype. This can be done in many different ways. For example, there could be a plant that wasn’t growing its roots and to figure out why, we would need to find all genes involved in the growing of the plants roots. The plant would need to be treated with some sort of mutagen, such as X-rays, chemicals, and insertional mutagenesis. X-rays can cause rearrangement and lose of DNA. Chemicals can result in point mutations, nonsense mutations, and non-coding regions. Insertional mutagenesis could effect the splicing of introns or where certain proteins bind/don’t bind affecting how they are coded. Then the plant would mate with multiple wild type plants. Each offspring of this plant would have different mutations. The plant(s) would be tested for the phenotype of interest or in this case, the ones not growing roots. This could …show more content…

A protein is present and can be translated back into a DNA sequence. This DNA sequence is then used to make a mutant gene. This mutant gene then makes a mutant phenotype. When the DNA sequence is formed it can be disrupted or changed by gene silencing, deletions and point mutations. Genes can be edited by being knocked out or knocked down. Two ways this can be done is by the use of RNA interference or morpholino for a specific gene, which are examples of gene silencing. This can inhibit the genes function and from there it could create mutated alleles, which could cause a loss of function phenotype. TILLING is also another way to mutate a specific gene using a lot of point mutations and chemical mutagenesis. This is done without injecting any foreign DNA into the genome and it produces differences in phenotypes to be looked

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