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Determination Of Flowering In Arabaliana

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Affects of Vernalization Length on the Flowering of Arabidopsis thaliana
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The flowering of plants is a tightly controlled process and flowering at the wrong time could cause detrimental problems for a plant, especially if it were to flower too early in the season. There are both environment and genetic factors that can influence flowering in Arabidopsis thaliana. In the winter-annual A. thaliana, environmental factors affect the gene expression of Flowering Locus C (FLC) (Michaels, et al., 2006). Environmental factors include the quality and quantity of light and vernalization, which is the promotion of flowering due to cold temperatures (Balasubramanian et al., 2006). Genetic factors include FRIGIDA (FRI), flowering …show more content…

In the Seo et al. study in 2009, it was shown that ten days of cold would slightly delay flowering, while twenty days of cold will delay it further. This means that the flowering of the plant is delayed according to the days it was in the cold temperatures. I hypothesize that A. thaliana will flower based on the first vernalization period, and will be unable to adjust to each variation of length in vernalization. If not, then one can assume A. thaliana is able to adjust the epigenetic effect on FLC that was caused by the first vernalization. If I shorten the vernalization time, the plant will flower based on the first vernalization, thus causing it to flower late. Likewise, if I lengthen the vernalization time, the plant will flower according to the first time, causing it to flower too early and potentially damaging the plant. This experiment may help us understand how global warming may affect winter-annual plants that work in a similar way to A. …show more content…

Two will be the control with a normal period of vernalization at five weeks, two will be in a short vernalization period at three weeks, two will be at a relatively long period of vernalization at seven weeks, and two will be at the longest period of vernalization of nine weeks. The independent variable will be vernalization length, and the dependent variable will be the time it takes the plants to flower. The vernalization will occur at 4ºC. Otherwise; they will be grown at 23ºC. Before vernalization, they will grow at normal temperature for four weeks. Other constants include light, at 20-150 µmol/m2sec and humidity, at 55%. The plants will be grown in long day periods of fourteen hours of light, with dawn at 0600 hours and dusk at 2000 hours. For accurate results, the plants will be grown in a growth chamber. After their first vernalization, I will grow them at 23ºC for four weeks, then put them in a vernalization state again, but each for one less week than previously done. I will repeat this same process twice so that I can shorten the vernalization time again. Flowering time will be measured in days since initial planting to the first

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