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Factors Of Seed Germination Of Raphanus And Cucumber

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Seed germination and seedling growth: The seeds were surface sterilized with 0.1% Mercuric chloride for 5 minutes then washed with distiied water several times to remove chemicals. The seeds of Raphanus and Cucumber were spread on sterile filter paper in each petridish and soaked in aqueous root exudates of each weeds. Each weeds had three replicates with equal quantity of seeds in petridishes. The seeds are allowed to grow for 10 days at room temperature. The seeds treated with sterile distilled water was termed as control. The seeds treated with weeds aqueous root exudates of Ageratum conyzoides, Leucas aspera, Scoparia dulcis, Spilanthes acmella and Vernonia patula were termed respectively as T1, T2, T3, T4 and T5. During the experiment period, care was taken to add equal volume of root exudates in each petridish periodically. After 10 days the seedlings were harvested. Then the effect of root exudates on the seed germination and seedling growth were studied. The shoot and root length of the seedlings were measured in cm and recorded. Then the average shoot and root length were calculated. The result observed are presented in Tables and photo plates.

RESULTS AND DISCUSSION A.Germination test: The aqueous root exudates of five weeds showed inhibitory effect on seed germination. The effects of germination inhibition were different for different weeds root exudates. The inhibition of germination was found acute in Cucumis sativus than Raphanus sativus. The seeds

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