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Heterogeneity in phenotypic characters is a widely observed phenomenon by breeder’s in all plant

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Heterogeneity in phenotypic characters is a widely observed phenomenon by breeder’s in all plant species were bud grafting technique is adopted as the popular propagation technique. Though these variations are attributed to several internal and external factors, genetic heterogeneity of the root stocks is considered as one of the major contributor. It is presumed that a series of responses called stock scion interactions are triggered as a result of the grafting procedure where a genetically diverse stock and scion are joined together to form a single plant. The impact of these interactions on several aspects of plant growth and development is an area under extensive investigation for the past many years (Rogers and Beakbane 1957; …show more content…

In this respect, earlier studies shows that, culture condition, callus phase, duration of the non morphogeneic stage and the growth regulators are some of the important factors influencing the induction of somaclonal variations in in vitro generated plants (Shepard et al. 1980; Hartman et al. 1984; Evans et al. 1987). As conventional micro propagation techniques using somatic explants requires the artificial induction of embryogenic competence in cells which are not naturally embryogenic, the influence of the above factors will be more in somatic embryogenesis process thereby increasing the possibility of generating more somaclonal variants (Dodeman et al. 1997). An alternative method is the zygotic embryogenesis technique where, the above effects will be negligible because the explant zygote is intrinsically embryogenic. In short, the zygote shows some structural and functional characteristics which are intimately linked with the formation of the first embryonic developmental stage with many of the genes required for the induction process already expressed. Due to this embryonic potentiality of the cells, their fate is already committed and does not need to be redirected towards a new developmental path. Considering the above facts, it is assumed that the chance induction of somaclonal

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