Macromolecules Lab Report

The purpose of this lab was to test different substances using various procedures to see what biomolecules were present and ultimately find out what restaurant Anna Lyza had eaten at before she died. For the first control test, we used vegetable oil to test for lipids. So, if the solution does not contain lipids, it does not become translucent when placed onto a paper bag square and held up to a light. So, it is a negative result. However, in the presence of lipids, the solution will become translucent when placed onto a paper bag square and held up to a light. Therefore in this case, the result is positive. On the other hand, we used albumin egg to test for proteins in another control test. If the solution does not contain proteins, it will not experience any color change and so it is a negative result. When there are proteins existing in the solution, it will turn bluish/purplish and for this reason it is a positive result. Furthermore in the third control test, we used dextrose to test for simple carbohydrates such as glucose. If the solution does not contain simple carbohydrates, it will not undergo any color change and will remain a blue color. So, it is a negative result in this circumstance. If there are simple carbohydrates present in the solution, the solution will turn reddish and so the result is positive. For the last control test, we used starch solution to test

All living things contain some form of organic macromolecules including: Lipids, proteins, carbohydrates and nucleic acids. All of these organic molecules are alike in the sense that is they are made up of bonded elements such as carbon, hydrogen, oxygen, and to smaller quantities nitrogen, phosphorus and sulfur. The macromolecules each contain large long chains of carbon and hydrogen atoms and often consists of repeating smaller molecules bonded together in a repeating pattern (polymers). To test whether a specific solid white substance is a protein is simple due to the unique chains found in the respective type of macromolecule. To test if the substance is a carbohydrate, two tests could be performed on separate samples of the object. First,

In doing the lab, one was able to determine the characteristics of the given solutions, containing different macromolecules, whilst doing the multiple tests. The tests performed were,

7)a) You would do the Benedict’s reagent test for simple sugars and the Lugol’s solution or Iodine test for polysaccharides and starch. To test the lipids you would use the Sudan iV test and Biurets reagent test for protein.

Analysis: In this experiment, every substance tested positive for at least 1 organic compound. Corn oil tested positive for lipids. Honey and oats tested positive for starch. Honey, apple juice, and unknown 1 tested positive for sugar. Egg white, gelatin, and unknown 2 all tested positive for proteins. So, not a single substance didn't test positive for at least 1 organic compound. All the indicators used in this experiment change color when in the presence of a compound; therefore, they chemically react with the compound. No errors were made during this experiment

2.List whether each of the following substances was positive or negative for reducing sugar, as indicated by the Benedict's test. (6 points)

Dehydration-synthesis is a reaction that occurs to form different types of macromolecules. It is a reaction that leaves a macromolecule structure along the lines of developing its own specific function in a living organism. The 4 most common ones are carbohydrates which includes monosaccharides and polysaccharides, lipids, proteins and nucleic acids (Mack 2012). Scientists identify different types of macromolecules to achieve a better understanding on how they function in our bodies. This can be achieved by studying their chemical properties and structure.

Milk pounder yielded a very light sheer orange at 80 degrees Celsius. For section B, Benedict’s test after hydrolysis several reducing sugars produced positive test results including glucose, sucrose, raffinose, starch and milk powder. That being said most positive results were organic in color however raffinose produced a brown color (closer to dark orange). Looking at the overall data, starch responded to both reagents for section B, Benedict’s after hydrolysis and section C, Lugol’s- meaning both were positive test results. However for section A, Benedict’s test before hydrolysis, Starch did not yield a positive result.

In this experiment, twelve liquids provided by the lab instructor are to be tested for the presence of reducing sugars, starches, as well as protein. After recording the results, repeated trials are conducted with the

The purpose of this lab was to identify the presence of macromolecules in the food we eat and include in our diet. The three different macromolecules we tested for include carbohydrates, better known as sugars and starches, lipids(fats), and proteins. The results of such tests were not surprising as the contents used, typically contained only one of the products tested for. For example, when I tested albumen(egg whites) for protein, it confirmed positive. In contrast, potato juice- another of the contents- did not contain enough protein for the test to prove positive.

We tested for simple sugars, starch, protein, lipids, and pH.

The objective in this experiment was to detect saccharides in different samples. The null hypothesis constructed is when a ketone and an aldehyde are in the presence of Benedict’s reagent or Lugol’s solution the appearance of the sample will change. The samples that were chosen to test consisted of distilled water, glucose, sucrose, bread, and salad dressing. The negative control of this experiment was water, and the positive control for this experiment was glucose. The independent variables of the experiment are the Lugol’s solution, and Benedict’s reagent. The dependent variable of the experiment are the ketone and aldehyde color change.

Name ____________________________ I) Introduction All cells contain four major types of macromolecules: carbohydrates, lipids, nucleic acids, and proteins. In today’s lab, we will be studying three of the four-proteins, carbohydrates and lipids. Various chemical tests can be used to detect the presence of each of these molecules. Most of the tests involve a color change visible to the eye. If a color change is observed, the test is considered positive. If the color change is not observed, the test is negative, indicating that a particular molecule is not present. In all the chemical tests we will be performing, we will also be using a control. In most cases, the control will be a sample of

Purpose the purpose of this experiment was to perform test to detect the presence of carbohydrates, proteins, lipids, and nucleic acids. Explain the importance of a positive and a negative control in biochemical test. Use biochemical test to identify an unknown compound.

In order to identify what food, and ultimately from which restaurant Parry ate the day of his poisoning, four experiments were carried out. Each experiment tested for the presence of the following four macromolecules in Parry’s vomit: