Pre Lab Report

In the Affinity Chromatography experiment we were purifying our Con A proteins. In general, affinity chromatography is a technique that is used for isolating a protein, in our case Con A from a large amount of other macromolecules. Our protein of interest is captured using a microbead matrix while we let everything else flow through the column. The Sephadex matrix is made of cross-linked glucose or dextran and because our Con A has an affinity for glucose it is able to bind to those beads. In general, we began by equilibrating our column with NaCl, then poured Jack Bean Meal Extract which so happens to contain Con A through our column, the Con A then binded to the Sephadex beads, and finally we eluded with a dextrose solution so that

Throughout the experiment, Allura Red was continually mixed with distilled water to form many different concentration solutions. The colorimeter was then used to determine the absorbance of each solution. Red light is around 650 nanometers on the visible light spectrum, but the colorimeter was set at 470 nanometers. This was done because Allura Red has the greatest absorbance when exposed to a light that is blue in color, which is why 470 nanometers was used.

Structure and mechanism of alkaline phosphatase. Annu Rev Biophys Biomol Struct. 1992; 21:441-83. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/1525473

The baseline is a common term for most chemical reactions. However, in this experiment, it is used to authorize a basic for a reaction. With the dependent variables being the substrate or the enzyme in this experiment, you have to point out and understand what is happening in the reaction. The baseline may vary with different situations that are applicable to the design of this experiment. In this experiment, the effects of changing a coincidental variable may possibly be resolved.

By looking at ‎Figure 3.7, a better match can be noticed in general shape of the profiles that are matching the theoretical profile corresponding to open-terrain condition (target α = 0.15). For instance, at d=2H, 3D-CFD-RSM is showing a good representation of open-terrain profile for the mean velocity until , and above this point the profile has a tendency to decrease speed which is also the trend of experimental measurements above . This is mainly because in the laboratory and also CFD simulations, there is no velocity inflow above the last upper row of fans, but for the theoretical formulation and in nature, there is still increasing velocity inflow beyond this height. Another insight of CFD simulations is that the results of the 2D k-є model are not as good as the results of 3D cases when comparing with the theoretical target velocity profiles.

Biochemistry is the study of chemical processes within and relating to living organisms. Amino acids play an important role in biochemistry because amino acids are found in living organisms in both their free forms and bound by amide linkages in peptides and proteins. Amino acids may either be regarded as the components which are found in proteins as they emerge from the ribosome or alternatively they may be considered as the components found in proteins which are sufficient age for post-ribosomal modification of some residues to have taken place.

I think this objective means that we need to make sure we understand the procedure or steps for the lab. Knowing the steps for the lab is extremely important because especially in chemistry if something is done out of order it can be dangerous. So recording the procedure in our lab book is important. This relates to my major of Political Science in that in Political Science, recording down things accurately is very important and if something isn’t recorded accurately it could be dangerous like in chemistry lab. In my lab report for the Density Lab I recorded the steps to the lab. The objective of that lab was to find out what procedure was preferable for finding density. This made it very important to makes sure the experimental procedure was recorded accurately.

This following science lab reflection is about light and how it travels and does its job. Before I talk about what I did in this lab, I will talk a bit about what I knew about light beforehand. I knew that when we look at an object, the light bounces off the object and hits the back of our eye, and we can see. This happens so quick, therefore it is hard to really notice it. I Predict that, because our nerves quickly sense what we are doing and looking at, especially the nerves in our eyes. Light rays and beams also cause radiation, which can significantly damage our eyes. This is about a couple things I know about light, but I learnt a lot more from the lab I conducted.

This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate student’s writing of lab reports by providing this information in an editable file which can be sent to an instructor

In this laboratory, the purpose of this lab was to allow the lab students to understand

Lastly, the individual flows were determined when all three lines in the closed conduit were opened.

The M-H loops taken at room temperature for Co0.6Zn0.4PrxFe2-xO4 (x = 0.0, 0.025, 0.05, 0.075, 0.10) compositions as a function of applied magnetic field H are shown in Fig. 8. From these loops, saturation magnetization (Ms), remanence magnetization (Mr) and coercivity (Hc) were measured, shown in Fig. 9. It can be observed that all the samples have the lower values of Hc (few hundred Oersted), thereby indicating the soft magnetic nature of these samples. Moreover, with thesubstitution of Pr3+ions, Hc of the samples enhances, whileareduction in MS and Mr have been observed.

introducing the parabolic profile for laminar flow in a pipe results in α = 2, for turbulent flow, we have α ≈ 1.0 and for uniform flow, α = 1.

Azam khan a, Umair Alam a, Danish Ali a, Detlef Bahnemann b, M. Muneer a,*

Abstract— A compact frequency reconfigurable slot antenna containing A U-shaped slot with short ends and an L-shaped slot with open ends are engraved in the ground plane to realize dualband operation By implanting 2 PIN diodes in tothe slots,accesible reconfigurability of three frequency bands over a frequency ratio of 2.62:1 can be achieved. For reducing the cross polarization of the antenna, one more L-shaped slot is introduced proportionally. In this proposed method, less VSWR, optimum return loss and high efficient radiation pattern will be obtained. Settled monopole bearing empty-headed regulations are achieved at all operating frequencies. From the simulated and measured results, we observe that the antenna is switched between 2