Name: Omid Hadj Drawer/Group #: 7
PS ID #: 1235832 Three digit mutant code: 168
BIOL 3311 Fall 2016 Lab Section: 16706 (Th2)
Date: 9/28/2016 TA Instructor Name: Laura Montier
BarH1 is critical for the proper development and patterning of ommatidia in D. melanogaster
Introduction
Although D. melanogaster is small and relatively inexpensive, its value to the research community and modern medicine is immeasurable. D. melanogaster is a great model organism for researching cellular processes of various diseases because within its genome, almost 70% of the human genome is conserved (Rubin 1988). Due to this similarity, the use of D. melanogaster has led to breakthroughs in the research of cancer, neurological diseases and various other diseases. For example, studying the development of photoreceptors in D. melanogaster eyes led to the identification of the Ras proto-oncogene pathway, which allowed researchers to gain a greater understanding of the signaling pathway and cellular process behind cancer (Tickoo and Russell 2002). Another example of the benefit of D. melanogaster as a model organism is the use of the mutant bang senseless and its known effect on voltage-gated sodium channels may lead to potential treatments for human epilepsy (Parker et al. 2010).
D. melanogaster is also an excellent model organism in studying embryological development due to its aforementioned similarities with the human genome. Certain homeobox genes found in the D. melanogaster
The goal of this study was to induce a deletion in the DMAP1 gene on chromosome two in Drosophila melanogaster through P-element mobilization. The DMAP1 gene may be an essential gene, however not much is known about it. We attempted to uncover the function of DMAP1 by creating a series of genetic crosses and selecting for brown-eyed non-stubble male flies that may have the deletion. To test whether these flies had the deletion, we produced PCR products and ran them on an agarose gel, which resulted as inconclusive. We created a balanced stock of flies homozygous for the deletion to see if the
Drosophila Melanogaster, commonly known as fruit flies, are highly important model organisms in pertaining to biological research. The logic behind their recurrent use is due to their: easy culture in the laboratory, brief generation time, and ability to produce large numbers of offspring. In this report, we created isolated virgin D. Melanogaster from the original three populations we were given and then created crosses between them. Upon observation, we noticed an unusual mutant that arose from two of the three created crosses. We suspected that this genetic mutation had previously been discovered and named.
Brennan K, Tateson R, Lewis K, Arias AM. A Functional Analysis of Notch Mutations in Drosophila. Genetics. 1997;147(1):177-188.
Hilde Mangold looked beyond the genetic makeup of embryos to find a small patch of tissue that was able to direct other cells to form an entire body plan. This is called the organizer. According to Shubin, “... many scientists consider Mangold’s work to be the single most important experiment in the history of embryology.” However, in the 20th century, scientists decided to try to understand why embryos of different species look the same and figure out more information, even beyond the organizer. Neil shares that scientists discovered a sequence of DNA within a few specific genes. The book shares more information on this sequence, stating, “ This little sequence is called a homeobox. The eight genes that contain the homeobox are called Hox genes.” Eventually scientists realized that the Hox genes are present in every animal with a body. Shubin tells of how hox genes establish proportions of our bodies too. They are responsible for the different regions of our head, chest, and lower back. Hox genes are involved with the development of different organs, limbs, genitalia, and guts. Shubin states that changes in our hox genes bring about changes in the way our bodies are put together. The similarities that are revealed between the basic body structures of different animals and creatures show how immensely diverse evolution
Roi1 is also known as rough eye and it is a dominant mutation which causes abnormal patterns and genomic inversions in the D.melanogaster eye (Chanut et al. 2002). The recombination map location of the Roi1 is 2-54.7. The gl3 is an allele and the Gl1 is a protein for the gl gene, also known as glass, both are located at 3-63.1. The gl gene is known to reduce the size of the adult D. melanogaster eye. Even though the gl3 is a weak allele for the glass gene it produces a really pigmented eye ( Ma et al. 1996). The rh1 gene is known as rhodopsin; the recombination map location is at 3.66.4. Rh1 causes degeneration of the D. melanogaster retina (Kristaponyete et al. 2012). Rho1 also known as the rhomboid gene, is closely related to the roughed (ru); which happens to be a recessive eye mutation (Wasserman
When examining the D. Melanogaster mutants in the lab, our group immediately noticed an apparent difference from the wild-type flies. None of the mutants were able to fly. This led us to believe that we were dealing with a wing mutation. Upon further examination, we concluded that it was the overall wing shape that prevented the mutants from flying. The wing shapes among the mutants varied in both size and shape. Some were long, while others were short. The mutant wings could be distinguished into two general classes. One division of the mutant wing was short and stubby, almost a fourth the size of the wild-type wing. The rest of the mutants ranged in wing size and length, however many mutant wings were the same length as the wild-type wings. Although the
The Drosophila melanogaster is an ideal organism most often used to study genes and mutations. The genome of the D. melanogaster, is similar to that of humans, making it the very beneficial to study. Through the studies done on the fruit fly, we are able to get a better understanding as to the processes of modern issues such as Alzheimer’s and cancer, in order to study and develop cures. Not only is the D. melanogaster an ideal organism based on its genetic similarities to human genetics,
In this experiment we bred drosophila with unknown genotypes, which are the actual genes of an organism, to see what the phenotype of their offspring would be. Introduction: T. H. Morgan was a geneticist who worked in the early part of the twentieth century. He was the first to use fruit fly, Drosophila melanogaster, as a model organism in genetic studies. He developed a name for himself that way and the science world wouldn’t be the same with out his discovery.
The Drosophila melanogaster is one of genetics most studied organisms. This is due to the Drosophila melanogaster being an excellent model organism. The Drosophila melanogaster has a short lifespan and is genetically similar to humans (Adams 2000). This experiment had three major goals. The first goal of this experiment was to determine which eye colors, body colors and wing type are dominant or recessive. The second goal was to determine if the gene for eye colors, body colors and wing type are on an autosomal or a sex chromosome. The third goal was to determine if eye colors, body colors and wing type are physically linked or independently assorting (Morris and Cahoon). First
As much as I would like to say it, transferring from a private school to a public school is honestly not a great deal. Trying to be picky with school selections was my first mistake as a rookie. In Omnia Paratus (GILMOIRE GIRLS), notice the dozens of attempts in repeating this three-worded line on the Opening Day for the heavily Scottish inspired school. Oh look, this revolutionised version of a primate fixated her physique, while the rolling stone gathers no moss. Nevertheless, Harambe had nothing on me. On rouge, I was taunted by the amount of people from primary school who transferred to private high schools. While, I was stricken to a state school. Subsequently. I figured that this aforementioned issue would result in no one attending mine.
Drosophila melanogaster, or the common fruit fly, is a holometabolous insect, meaning it has larval and pupal stages before becoming an adult. At the beginning of development, the D. melanogaster eggs go through syncytial development, which allows for a unique pattern of development. Furthermore, as the egg develops it is surrounded by a thick layer called the chorion, and as two respiratory filaments and a micropyle. Once the egg develops into a larva, it goes through a first, second and third instar stage. In the third instar stage, the imaginal discs and salivary glands develop. The imaginal discs are pockets of epithelium cells that form the adult structures of the organism, including the wings, halters, legs antennae and eyes. The salivary glands are large cells that contain large polytene chromosomes that can be observed when the the glands are stained and squashed.
Introduction: The Drosophila project was fundamentally conducted to determine potential regulators of the wingless pathway in Drosophila Melanogaster. All the experiments in this project were used to establish two things. Firstly, the location of the transposon insertion and secondly, the effect the genes near the transposon insertion had on the wingless pathway.
Homeotic genes are like a “tool kit” like the statement says. Homeotic genes determine and direct the formation of body structures in an embryo. These genes are like a “tool kit” since it contains all the “tools” or in this case a homeobox that have a DNA sequence that encode amino acids. This then helps the homeotic genes’
The fruit fly Drosophila melanogaster is an extensively studied model organism. Its steadfast presence since the early 20th century, has led to significant findings and contributions in classical genetics, various areas of biological sciences, and biomedical research. This includes the discovery of the fundamental concept that chromosomes carry heritable traits (Pandey and Nichols, 2011). Its fully sequenced and annotated fly genome, homology similarity to the human genome, rapid life cycle, large number of progeny, easiness of culturing, and low maintenance expenses are the primary attributes that make it an excellent model organism convenient for study (Arias, 2008; Pandey and Nichols, 2011). Even more so, humans and D. melanogaster share
The cause of Retinitis pigmentosa is due to the harmful changes in any one or more than 50 genes. The genes are carried by instructions that make proteins that requires, among the cells in the retina, called photoreceptors. Some of the developments, or mutations, within the genes are relentless that the gene cannot produce the necessary protein, which limits the cellís function. The mutations produce a protein that toxic the cell, and the other mutations leads an abnormal protein that doesn’t function properly. These essentials result, in which damage has been done in the photoreceptors within the retina of the eye.