Discussion – Effect of pH on the Function of the Enzyme Amylase
The objective of the lab was to examine the effects of environmental variables on the functions of an enzyme. To the point, an experiment was conducted to test the effect of pH on the function of the enzyme Amylase. Enzymes are proteins that act as catalysts and help reactions take place. In short, enzymes reduce the energy needed for a reaction to take place, permitting a reaction to take place more easily. Some enzymes are shape specific and reduce the energy for certain reactions. Enzymes have unique folds of the amino acid chain which result in specifically shaped active sites (Frankova Fry 2013). When substrates fit in the active site of an enzyme, then it is able to catalyze the reaction. Enzyme activity is affected by the concentrations of the enzymes and substrate present (Worthington 2010). As the incidence of enzyme increases, the rate of reaction increases. Additionally, as the incidence of substrate increases so does the rate of reaction. Almost all enzymes need specific conditions for them to function. The conditions include temperature, pH level, and concentration of salt. Enzymes have optimal conditions. If they are changed, the enzyme may denature and deactivate. If that happens, the enzyme would not be able to catalyze the reaction, and the reaction rate would decrease (Worthington 2010). Amylase is a digestive enzyme. The enzyme is found in the salivary glands in your mouth
Enzymes are biological catalysts, which means it decreases activation energy in reactions. The lower activation energy in a reaction, the faster the reaction rate. Many enzymes alter their shape when they bind to the activation site. This is called induced fit, meaning for the enzyme to work to its full potential it has to change shape to binding substrate. The location of enzyme’s activation site is on the surface of the enzyme, where the binding of substrates take place. Enzyme activity can be influenced by a variety of environmental factors. If the concentration of enzyme is low, and there is a great deal of substrate, then increasing enzyme concentration results in more molecules available to convert substrates to products. Thus, increasing enzyme concentration can increase reaction rate. If substrate concentrations are low, and many of the existing enzymes are idle because of a lack of substrate, then adding enzyme will have no effect on reaction rate. Enzyme concentration affects the enzyme activity, because the more enzyme concentration the faster the reaction rate, until it hits it’s limiting factor. When substrate concentration is increased, it also increases rate of reaction. Temperature plays an important
Enzyme catalysis is dependant upon factors such as concentration of enzyme and substrate, temperature and pH. These factors determine the rate of reaction, and an increase in temperature or pH above the optimum will
Enzymes are biological catalysts that speed up chemical reactions, without being used up or changed. Catalase is a globular protein molecule that is found in all living cells. A globular protein is a protein with its molecules curled up into a 'ball' shape. All enzymes have an active site. This is where another molecule(s) can bind with the enzyme. This molecule is known as the substrate. When the substrate binds with the enzyme, a product is produced. Enzymes are specific to their substrate, because the shape of their active site will only fit the shape of their substrate. It is said that the substrate is complimentary to their substrate.
Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment. Whether or not the reaction continues to move forward is not up to the enzyme, instead the reaction is dependent on a reaction’s free energy. These enzymatic reactions have reactants referred to as substrates. Enzymes do much more than create substrates; enzymes actually work with the substrate in a reaction (Madar &Windelspecht, 106). For reactions in a cell it is
Enzyme is an essential concept in biology; they act as catalysts, speeding up chemical reactions by lowering the activation energy. According to the Lock & Key model, a specific substrate joins with a specific enzyme at the active site, to form the enzyme-substrate complex; this brings the reactants closer together to facilitate chemical reactions (Ophardt).
“Enzymes are proteins that have catalytic functions” [1], “that speed up or slow down reactions”[2], “indispensable to maintenance and activity of life”[1]. They are each very specific, and will only work when a particular substrate fits in their active site. An active site is “a region on the surface of an enzyme where the substrate binds, and where the reaction occurs”[2].
Amylase is a digestive enzyme. The enzyme is found in the salivary glands in your mouth and cells in your
The purpose of this experiment is to determine how the acidity and basicity of the environment affects the rate at which a specific enzyme is able to produce products in a computer simulation.
This mixture of amylase and pH buffer will be modified by the addition of more pH buffer into water down (diluted) amylase enzyme. Also, the pH buffers used in this experiment vary by 1, 4, 7, 10 and 14. Dependent Variable: Rate of starch hydrolysis, which will be determined by the change of color; if the iodine turns blue- black it indicates that the enzyme is denatured and if the iodine turns orange-yellow it indicates that the enzyme is working properly by digesting the starch. Controlled Variables: • Amount of iodine.
Enzymes are small biological molecules that significantly speed up the rate of chemical reactions. Enzymes are a vital element for life and serve a vast amount of important functions inside the body, such as aiding in digestion and metabolism. Some enzymes help break down larger molecules into smaller pieces that can be easily absorbed by the body. Enzymes are highly selective catalysts, which means each enzyme catalyzes only one type of reaction. This occurs because substrates with which an enzyme acts, has a special that compliments the enzymes active site, fitting together like pieces of a puzzle. There are two models in regards to how the enzyme and catalyst fit together, the lock-and-key model and the induced-fit model. In the lock-and-key model, the active site of an enzyme is specifically designed to fit with specific substrates. In the induced-fit model, the active site and substrate don't fit perfectly together but instead, they both can alter their shape in order to connect. PH, Temperature and Concentration affect the rate of enzyme activity. Most enzymes would denature when exposed to extreme acidic or basic PH. As temperature rises molecules will have a greater kinetic
Observe and record what happens when enzymes (chicken) are added to different amounts of acid (hydrogen peroxide) mixed with water. The activity level of these enzymes will differ and be dependent upon several factors, such as consistency, size and shape of the molecule. Specific factor will be the concentration enzyme and substrate. I will conduct a controlled experiment to determine the influence of pH on the enzyme catalase and the release of oxygen.
: Enzymes are made up of proteins and their function is mainly determined by their structure. The primary structure of an enzyme is determined by the sequence of amino acids. The secondary structure is based on the folding of the primary structure. Lastly, the tertiary structure is based on the folding of the secondary structure and its interactions of the side chains. The purpose of an enzyme is to speed up chemical reactions in a cell. Temperature is a factor that affects enzyme activity because as temperature increases the enzyme reaction increases too. Also, pH levels also play a role in affecting enzyme activity. Very high or very low pH levels can result in the loss of enzyme activity. For an enzyme to maintain activity it needs to be
Some of them include temperatures, pH and amount of reagent. In our experiment, we decided to carry a test on the effects of low pH on enzyme activities. The objectives of the experiment is to find if lowering the PH will lower the activities of enzyme catalase. A hypothesis was then formulated. Hypothesis Low pH reduces the rate of activities of enzymes catalase.
Enzymes are globular proteins that act as catalysts which affect the rate of chemical reaction without being destroyed or altered during the process. They are very effective in the body systems of organisms, an enzyme that catalyzes more than one thousand chemical reactions per second. But there are certain conditions that enzymes can work normally or efficiently, such as the temperature of the environment must be correct for each enzyme because different enzymes will have different optimum temperature ranges in which they can live; pH levels in the environment must also be correct because if the environment around the enzyme is too basic or acidic, the enzyme will quickly denature; substrate concentration also affects the rate of reaction
Thousands of enzymes have been identified, suggesting that every function or substrate has a specific enzyme those catalysis unique chemical reactions. However both physical and chemical factors can interfere with enzymes, this is partially due to the fact that enzymes construct their catalyst reactions under specific environmental conditions.