Q: Michelle has a clone of the DNA of a newly discovered virus. She wants to identify which specific…
A:
Q: gene therapy
A: Gene therapy - it's a therapy in which doctor replace the damage genes by making it in vitro. It…
Q: Draw an Illustration of the steps in restriction digestion and PCR [ Please make it clean and…
A: Restriction digestion is the process of cutting the DNA sequence at a particular nucleotide sequence…
Q: has been assembled by researchers and transplanted into a donor bacterial strain to study never…
A: there are diverse bacterial strains that have an antibiotic resistance system. Using antibiotics may…
Q: PCR has many useful applications. However, an incorrect application of PCR is: a) Amplification of…
A: DNA or Deoxyribonucleic acid is a macromolecule which is responsible for the transfer of hereditary…
Q: The genomic DNA of a bacterial cell is not destroyed by the cell's own restriction enzymes because…
A: None of the above Restriction enzymes are the enzymes which cut DNA at a specific location called as…
Q: PCR can be utilized _______
A: Answer: Option A PCR can be used for cloning vectors
Q: Taq Polymerase that is used in PCR is an artificially engineered enzyme created by scientists, which…
A: Taq in the name Taq polymerase, stands for Thermus Aquaticus, a microorganism which has evolved to…
Q: Which of the following is NOT an advantage of the PCR method over traditional cloning methods for…
A: PCR or polymerase chain reaction refers to the method by which a particular DNA (deoxyribonucleic…
Q: what questions should be asked to identify how biotechnology plays a role in economics and society…
A: Biotechnology refers to the application of science to engineering, molecular biology, genetics, and…
Q: Why is Covid PCR testing also referred to as reverse transcriptase? O because it is done real-time O…
A: PCR (Polymerase Chain Reaction) is a technique, which is used to amplify a small fragment of DNA.…
Q: describe in your own words what DNA barcoding is. Explain how PCR is used in this process
A: The process of determining the sequence of nucleic acid is called DNA sequencing. This includes…
Q: We have mRNA prepared from human cells but PCR needs DNA. What should we do?
A: A polymerase chain reaction (PCR) is a technique that is used in molecular biology to amplify a…
Q: The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy.…
A: DNA cloning is a process in which clones / copies of DNA are formed . DNA sequencing is a technique…
Q: ___ can be used to introduce foreign DNA into cells of tissues to cure inheritable diseas
A:
Q: 1. You are a research assistant in a renowned science academy. Your supervisor requires you to…
A: PCR:- it is used to amplify the fragments of a DNA or gene of interest. Gene cloning:- it is a…
Q: Which of the following is NOT a vector used in genetic engineering? O A. mosquitos O B. viruses OC.…
A: Foreign DNA is delivered into recipient cells using genetic vectors. Vectors can self-replicate and…
Q: What are the applications of pcr in BIOTECHNOLOGY RESEARCH?
A: PCR (Polymerase chain reaction) is a repeated cycle through which a segment of DNA is multiplied by…
Q: CRISPR has the potential to change everything. What potential benefits of this technology are you…
A: CRISPR stands for clustered regularly interspaced short palindromic repeats. It is a technique…
Q: Sequencing the human genome, the development of microarray technology, and understanding of complex…
A: Proteomics is the large-scale study of proteins.Proteomics has enabled the identification of ever…
Q: The southern blot below is the results of a PCR-based paternity test conducted at KNUST forensic lab…
A: The genetic material of an individual is packed in the form of DNA. Shorter nucleotide sequences…
Q: Genes of Interest can be selected from a genomic library by using (a) cloning vectors o) DNA probes…
A: Vector refers to a DNA molecule which is used as a vehicle to carry genetic material from one cell…
Q: The genome of a typical bacterium contains about 5 x 106 base pairs, and can be replicated in about…
A: Replication is the process of synthesis new daughter DNA strand using the existing parental DNA…
Q: Which of following can you do with the biotechnology depicted in the figure? The physical The…
A: DNA fingerprinting also called DNA typing, DNA profiling, genetic fingerprinting, genotyping, in…
Q: PCR is quick, efficient and easy to perform. However, there are some situations when cell-based…
A: PCR which stands for polymerization chain reaction is an analytic technique which is used to amplify…
Q: Give me 3 examples of how PCR and restriction enzymes can be used to create a genetic fingerprint
A: DNA is made of nucleotides, which are composed of a nitrogenous base, deoxysugar, and phosphate. The…
Q: When you have a recombinant organism, it generally has genetic information from? only a bacteria…
A: Recombinant organism – An organism that contains a different combination of alleles from either of…
Q: • High-throughput technologies allow parallel sequencingof millions of individual DNA molecules.…
A: High throughput methods are used in cell biology. Cell biology is a division of biology that deals…
Q: MOLECULAR CLONING • Molecular cloning is the laboratory process used to create recombinant DNA. • It…
A: Recombinant DNA: it is a method of combining two distinct DNA (molecule) sequences, which may or may…
Q: Cloning is a general term used for whole organisms and DNA sequences. Define what we mean when we…
A: DNA is the genetic material present in most of the living organisms. The DNA is made up of 4…
Q: The following statements are true about common gene cloning procedures except: -DNA plasmids can…
A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…
Q: CRISPR-Cas9 technology can edit the DNA in random regions True False
A: CRISPR Cas9 technology can edit DNA in precise specific locations and not rand DNA regions. CRISPR…
Q: The use of genetically modified organisms in areas of agriculture and medicine is favored by many.…
A: Genetically modified organisms (GMOs) are the organisms having altered genome by using Recombinant…
Q: Genetic Methods to modify genes Replacement of genes (recombination) Creates genetically modified…
A: answer in picture format:
Q: The idea behind PCR-based diagnostics is that a very small number of microbial genomes in a patient…
A: A primer is a short nucleic acid sequence that provides a starting point for DNA synthesis. In…
Q: has been assembled by researchers and transplanted into a donor bacterial strain to study never…
A: A- recombinant plasmid B- developmental genetics
Q: To study the function of any gene of interest you would perform the loss and gain of function…
A: Genes : It can be defined as a segment of DNA that codes for a specific protein performing a…
Q: If a researcher attempted to do a PCR amplification of the slc24a5 gene in the gol1 mutatant line,…
A: PCR Stands for polymerase chain reaction and is used in the amplification of DNA It has three steps…
Q: Definition of Terms( This is all about Applications of Recombinant DNA) a. Clone b. Plasmids c.…
A: Dear student, we are authorized to answer three subparts at a time, since you have not mentioned…
Q: It is difficult to use a restriction enzyme that cuts (shown as ) within one of these restriction…
A: Ans- It is difficult to use a restriction enzyme that cuts like the sequence given in option D( *…
Q: The Human Genome Project attempts to modify organism’s DNA inserting desired genes into bacteria.…
A: The human genome project (HGP) aims to sequence the whole human genome (3 billion base pairs)…
Q: Why is CRISPR useful for genome editing? O it can't be used in humans, but it works really well in…
A: Answer:- Option D The gene editing is a recent concept. It allows cutting the DNA sequence at a…
Q: If a police detective finds the tiniest amount of cells at a crime scene, they could produce more…
A: Polymerase chain reaction Hence option(b) is correct.
Q: 177 3 The figure above represents results observed from the differential gene expression lab. Lane 3…
A: PCR is the polymerase chain reaction. This is the molecular technique used for amplification of the…
Q: Scientists can distinguish between DNA of different individuals, thus making this information useful…
A: Molecular Biology approaches are used to investigate the molecular foundation of biological…
Q: Which of the following does NOT require a primer? A) PCR-based viral test B) cloning a human gene…
A: It is the process of producing individuals with identical or virtually identical DNA, either…
Q: Which of the following term is associated with DNA finger printing?a) Hybridomab) Site specific…
A: DNA fingerprinting - DNA fingerprinting is a kind of technique which is used to make or identify the…
...... doesn't use for gene expression at global level
Microarrays
maldi tof mass spectrometry
quantitative pcr
none of wnove
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- Wa... SLSU SVM Status... X ↓ O 650 O 550 O 500 200 O 850 Smal ↓ O 1550 Applicant Dashbo... 300 Msel 350 Probe Smal ↓ Caption: Restriction enzyme sites are indicated by the arrows. Smal binds to CCCGGG sequences. Msel binds to TTAA sequences. Enzyme 'X' refers to an unnamed restriction endonuclease that is not affected by DNA methylation. The distance between enzyme sites is indicated as number of bases. Assume only CpG methylation occurs. A human genomic segment (depicted above) was subjected to various experimental conditions (enzyme digestion, DNA methylation) AS INDICATED BELOW. 200 If the DNA was not methylated and digested with enzymes X, and Smal, what is the largest DNA fragment size that would show up on a Southern blot using the radiolabeled probe? Smal ↓ 500 MacBook Pro X ↓Why is it important to sequence positive clones derived from PCR cloning? Group of answer choices Errors could have been incorporated during restriction enzyme digestion and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during plasmid DNA extraction and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during PCR and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during cloning and so it is important to verify that only the expected protein is produced.BOTTOM The sequence depicted on the gel is 5'-GTGATGTAG-3' The sequence depicted on the gel is 3'- GTGATGTAG -5' The sequence shown on the gel is the sequence of the template strand of the reaction The sequence shown on the gel is complementary to the template strand of the reaction The template strand of the reaction is antiparallel to the sequence shown on the gel The largest fragment shown on the gel is closest to the top The largest fragment shown on the gel is closest to the bottom Each of the fragments shown on the gel have a primer incorporated at their 5' end Each of the fragments shown on the gel have a primer incorporated at their 3' end The number of nucleotides in the shortest band on the gel is 21 The number of nucleotides in the longest band on the gel is 21 ick Save and Submit to save and submit. Cick Save All Answers to save all answers.
- Select all that would be true if I had a missense mutation in an gene: The missense mutant protein would be the same size by Western as the wildtype protein The missense mutant allele would be a different size compared to wildtype by PCR- electrophoresis The missense mutant protein would be a different size by Western compared to the wildtype protein The missense mutant allele would be the same size as wildtype by PCR-electrophoresis....... assess anatomical distribution of gene expression. genomic pcr nortbern blotting Rnai in situ hybridizationAll things considered, the most important factor with respect to successful PCR is Multiple Choice primer design annealing temperature extension time quantity of template
- Our PCR samples already contain loading dye, but sometimes this isn’t the case. If your samples didn’t already contain dye and you wanted to load your PCR sample onto an agarose gel, you’d need to add loading dye to the proper concentration. There is a 6X loading dye available for use; how many µl of this loading dye will you add to 10 µl of your sample so that it is at a 1X working concentration? Show your work.What would be the expected effect on the PCR reaction, if you increased the temperature of the annealing phase and the length of the elongation phase? O Accuracy will be reduced, but yield will be increased Accuracy and yield will be increased OAccuracy will be increased, but yield will be decreased Accuracy and yield will be reducedO e. Gene amplification CLEAR MY CHOICE To study the function of any gene of interest you would perform the loss and gain of function approaches by either deleting or re-expressing the gene of interest, which of the following can be used to determine and quantify the activity of the gene? Oa. Western blotting O b. Gene knockdown O. PCR/OLA O d. Microscopy O e. DNA hybridization Paternity testing can be detected most precisely by using technique
- Make the PCR Cocktail I field out my work.. but I don't know which one is incoeert.. This table lists the ingredients, stock reagent concentrations, and concentrations in the PCR reaction. Prepare a "PCR cocktail" to be added to your samples to achieve these concentrations. Make enough cocktail to run nine samples. [Four student samples + three positive controls + one negative control + one extra.] {Hint: Remember that the concentration in the reaction is not the same as the concentration in the cocktail!] Component Stock Concentration Concentration in the PCR reaction Volume per reaction Volume to make cocktail Sterile water - - 0µl 0µl PCR buffer w/ MgCl2 10x 1x 4µl 36µl Nucleotide mix 10 mM 0.2 mM 0.8µl 7.2µl Primer 1 (Forward) 10 µM 1.0 µM 4µl 36µl Primer 2 (Reverse) 10 µM 1.0 µM 4µl 36µl Taq DNA polymerase 5 U/µl 1.0 U 8µl 72µl DNA template (sample) - ~1 ng 20 µl 180µl Total - - 40 µl 360 µlOO HUAWEI Nova 2 Plus DUAL CAMERA estion To study the function of any gene of interest you would perform the loss and gain of function approaches by either deleting or re-expressing the gene of interest, which of the following can be used to determine and quantify the activity of the gene? red Oa. Microscopy d out of O b. Western blotting O C. PCR/OLA on O d. Gene knockdown O e. DNA hybridization stion Which one of the following is NOT correct regarding Bacterial Biosensors? O a. produced by all Pseudomonas species ed O b. Encoded by LacZ gene out of O c. Encoded by Lux genes O d. Chemical compounds used for the quantitative assessment of water pollution O e. In the presence of pollutants the bioluminescent decreases. stion CD4-Pseudomonas Exotoxin Fusion Protein is another biotechnology strategy for HIV therapeutic setting. This construct will target and kill: Select one: d O a. Any lymphocyte put of O b. HIV (virus only) O C. Non infected TH O d. Infected TH cells O e. Infected TH…at Arrange Window Help 4) 57% D Tue 4 May Tools Slide Show 2 ARMS PCR nations Slide Show Review View A A =、=<|EE|三v| v v A Sha v 20 Quick Styles D Sha Text Arrange Convert to SmartArt Picture Shapes ab x x. AV v Aa v D. Av A、申。 Box Briefly outline how MLPA probe-sets can simultaneously detect multiple targets using a single set of PCR primers. E Notes Comments MAY 4 Pr w MacBook Air
