1. (2.5 pts) A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as diagrammed below. Each dilution tube contained 400 µl of diluent and 100 µl was transferred into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes. a. What is the dilution between each tube shown in the diagram below? Express your answer as a ratio. b. What is the total dilution of tube number 5? Express your answer as a ratio. c. What is the concentration of viable bacteria in the original culture? Express your answer using scientific notation and the units CFU/ml. d. What is the concentration of viable bacteria in tube number 2? Express your answer using the units CFU/ml. e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would you expect to see after the plate was incubated? 2 3 423 80 13 Number of colonies
1. (2.5 pts) A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as diagrammed below. Each dilution tube contained 400 µl of diluent and 100 µl was transferred into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes. a. What is the dilution between each tube shown in the diagram below? Express your answer as a ratio. b. What is the total dilution of tube number 5? Express your answer as a ratio. c. What is the concentration of viable bacteria in the original culture? Express your answer using scientific notation and the units CFU/ml. d. What is the concentration of viable bacteria in tube number 2? Express your answer using the units CFU/ml. e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would you expect to see after the plate was incubated? 2 3 423 80 13 Number of colonies
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 2.4P
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Transcribed Image Text:1. (2.5 pts) A pure bacterial culture of unknown concentration was diluted to determine the
concentration of viable bacteria in the original culture. Serial dilutions were performed as
diagrammed below. Each dilution tube contained 400 ul of diluent and 100 ul was transferred
into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes.
a. What is the dilution between each tube shown in the diagram below? Express your
answer as a ratio.
b. What is the total dilution of tube number 5? Express your answer as a ratio.
c. What is the concentration of viable bacteria in the original culture? Express your
answer using scientific notation and the units CFU/ml.
d. What is the concentration of viable bacteria in tube number 2? Express your answer
using the units CFU/ml.
e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would
you expect to see after the plate was incubated?
1
2
3
5
423
80
13
Number of colonies
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