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- Briefly explain the principle behind flow cytometry What is/are purpose of flow cytometry?explain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork. 1. Pulsed-Field Gel Electrophoresis (PFGE)2. Whole Genome Sequencing (WGS)Match the following experimental methods with their specialty procedures and objectives. autoradiography use of green fluorescent protein (GFP) fractional centrifugation use of genetic mutants A. silencing genes means inhibition of the expression of a protein B. fluorescing GFP protein is fused with a protein tracked in the cell C. biochemical processes are visualized using radiolabeled materials D. tissue samples homogenized at varying centrifugal force leads to isolation of desired cellular fraction
- Following is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…The following are words used in animal testing and its alternative methods. Define the following terms - a. Vivisection b. Optogenics c. In vivo d. In vitro cell culture e. In silico computer simulation f. Pharmacokinetics g. Pharmacodynamics h. Tissue culture i. Phototoxicity j. 3D cell culturesList 3 different types of nanoparticles that can be used in drug delivery applications? List down the 3 different goals of drugdelivery systems
- explain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork. (1) Restriction Fragment Length Polymorphism (RFLP)(2) Pulsed-Field Gel Electrophoresis (PFGE)(3) Whole Genome Sequencing (WGS)(4) Gram Staining(5) Biochemical Reactions: Multiple Tube Fermentation Technique(6) Biochemical Reactions: IMViC Test1. Labels are used in biosensors to amplify a biorecognition event. Which is frequently used in lateral flow assays? C-13 or P-32 nanoparticles fluorophores labeled enzymes 2. Why is it a good idea to use filters? Because of the mains interference All of these Because of the parasitic Because the signal is mixed with other unwanted signals•There are two figures- one for vector control plate & another PCRinsert+vector experimental plate. Control plate = 10 blue colonies , Ligation plate ( experimental) = 150 white , 8 blue. Interpret what each figure means, what is its importance (control and experimental)? Why do colonies grow on vector control plates? Why are both blue and white colonies in your experimental plate? What were the components of the agar plate & their use (AMP+Xgal+IPTG)? What do these suggest about amp sensitivity and resistance? Why did we use X-gal and IPTG? Can you think of any issue with your result? Is the recombination efficiency good? Is your transformation efficiency good? •Write a concluding sentence about this lab and the data you analyzed.
- With detail, compare and contrast the following 5 real-time assays; Taqman, SYBR Green, Molecular Beacon, FRET, and Scorpion. Please write complete sentences in paragraph form. Identify how the assays are similar but more importantly, what are the distinguishing features of each of the technologies.1.Discuss how PCR may be used for the detection of disease-causing pathogens in a population during the COVID Pandemic. For example: it may be used to check if a patient has a COVID virus infection. 2. Discuss how the cloning and expression of certain genes allows for massive production of the desired product. For Example: the cloning and expression of insulin in bacteria allows for the mass production of this necessary protein for use by diabetic patients.Explain your opinion about how \ SPR technology should be used.
