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- Select ALL the correctly matched terms: a. Immersion oil/ Oil with a different refrative index than air, used to enhance magnification b. Luminescence/ Absorption of light and energy within a sample c. Fluorescence/ Absorbed light rays are changed into longer wavelengths and re-emitted d. Diffraction/ Bending of light rays around an openingParagraph 3. If the ocular lens on a microscope has a magnification of 20OX, what is the total magnification of: a. a scanning lens with magnification of 4X b. a low-power lens with a magnification of 1oX c. a high power lens with a magnification of 40X d. an oil-immersion lens with a magnification of 100X 4. Choose how the letter "R" will appear as viewed under a microscope. R B R As viewed with the naked eye b. a. P. 5. True/False a. Begin the focusing process with the high-power objective. b. Store the microscope with the oil-immersion objective in position. c. Use tissue d. The coarse adjustment knob can be used with any objective lens. раper and water to clean the ocular and objective lenses.Compute the total magnification. [Use 10x magnification for eyepiece]1. Scanning2. LPO3. HPO4. OIO
- 3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. A.The cell is being viewed under high power. Scanning power objective=4x; Low power objective= 10x; High power objective= 40x; Eyepiece= 5x; Low power field of view (FOV)= 4.2 mm3. Please number these steps, in the order they should be completed when using a microscope. Remove specimen, lower stage, wrap cord, and return covered microscope to numbered shelf. Move to other objective lenses to increase magnification. Rotate lowest-magnification objective lens into position (probably 4x or 10x). Clean all lenses with lens paper. Place specimen on stage. Adjust chair to a comfortable height. Use coarse focus knob to adjust stage until an image is visible. Be careful to use only the fine focus knob with the longest objective lenses.B. Write the function/s of each part of the microscope listed below. a. Eyepiece b. Draw tube c. Hemispheric prism housing d. Dust shield e. Revolving Nose Piece f. Objectives: Scanner LPO HPO ΟΙΟ g. Arm h. Coarse Adjustment knob i. Fine adjustment knob j. Slide holder & clip k. Rear knob I. Front knob m. Central aperture n. Condenser o. Iris diaphragm p. Mechanical Stage q. Mirror r. Mirror rack s. Stage adjustment knob t. Base
- Preparing a wet mount of the letter "e”. 1. Obtain a prepared ‘e’ slide. 2. Focus the slide first with the scanning objective, then click to lower power and focus again. 3. Finally, focus the slide under high power. Remember, at high power, you should ONLY use the fine adjustment knob. 4. Draw the ‘e’ exactly as it appears in your viewing field for each magnification. The LPO, HPO, and Oil ImmersionWhich of the following is not required for a UV-vis absorption spectrophotometer? Group of answer choices camera detector monochromator light sourceMatch the term with it's definition from the lab Group of answer choices Field of View [ Choose ] occular lens x objective lens the width of the circle of light the distance from the slide to the bottom of the lens clarity of an image Working Distance [ Choose ] occular lens x objective lens the width of the circle of light the distance from the slide to the bottom of the lens clarity of an image Resolution [ Choose ] occular lens x objective lens the width of the circle of light the distance from the slide to the bottom of the lens clarity of an image Total Magnification [ Choose ] occular lens x objective lens the width of the circle of light the distance from the slide to the bottom of the lens…
- The significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.Preparing a wet mount of the letter "e”.1. Obtain a prepared ‘e’ slide.2. Focus the slide first with the scanning objective, then click to lower power and focus again.3. Finally, focus the slide under high power. Remember, at high power, you should ONLY use the fineadjustment knob.4. Draw the ‘e’ exactly as it appears in your viewing field for each magnification of Oil Immersion objectiveWhat part of the microscope adjusts the amount of light projected onto the slide? Group of answer choices A. rheostat B. on/off switch C. iris D. course adjustment