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- In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.You have carried out an experiment using the spectrophotometry concept. A solutioncontaining compound X is mixed with reagent 1 and then reagent 2. This mixture produces ablue colour whose absorbance (A) could be read at 550 nm. The results are shown below. If the standard solution (compound X) used have a concentration of 1 mM:1. Calculate the quantity of the standard compound X in μmoles for each test tube (1-7).In basic spectrophotometer, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of a light beam is transmitted through 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 cm-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 0.025 mg/mL. and compare between 1 and 2
- The BSA stock solution from the previous problem was then diluted to generate a set of standard solutions of known concentrations. After performing biuret assay on these solutions, their absorbance at 540 nm were measured using a UV-vis spectrophotometer. The following data were obtained. Concetration of BSA Absorbance (mg/mL) 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8 0.380 1.0 0.485 Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.7. The spectrophotometer is used to measure absorbance experimentally. Briefly, explain how this is accomplished.What are 2 advantages to using absorbance values (from a Spectrophotometer) over a spread plate and dilution factors? What are 2 disadvantages?
- The significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.2. Imagine you have mixed the following known concentrations of a solution. You then measured their absorbance in the spectrophotometer. Construct a standard curve (a line) from the following data points. Use the graph below. Each axis must be labeled with the variable and units, and the scale must be evenly spread. Tube Known concentration (M) Absorbance (AU) 1 0. 0.1 0.03 0.5 0.06 1.0 0.08 5 5.0 0.28 Eseel 221.. What is the useful operating volume range of a Gilson P-10 pipette? Where did you find this information? 2.. Which micropipette should be used to dispense 4.50 x10-4 L of water? Explain your choice. 3. What should 2.50 x 10-4 L of water weigh at 27 °C? (Show calculations and include proper units with your answer) 4. Determine the temperature in the room where a gravimetric pipette calibration is being conducted. A 100 μl volume yielded a weight of 99.46 mg. Show your work for full credit.
- Match the following items: [4] 1. 540 nm A. Wavescan 2. Quantity of light of a specific wavelength absorbed by a sample B. Standard series 3. Reference samples with a concentration range C. Absorbance 4. Absorbance values at all wavelengths D. Purple(b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 5 10 15 fluorescence intensity 300000 250000 200000 150000 100000 50000 0 0 Table Q1 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte]/nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or…Answer this ff question: 1. Explain the importance of system suitability on HPLC method of analysis. 2.In UV and visible spectrophotometry, the specimen is generally dissolved in a solvent, and determinations are made at room temperature using a path length of 1 cm. Give the most common solvents suitable for UV or visible spectrophotometry.