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- A protein’s concentration can be determined spectrophotometrically by mixing it with an indicator called a Bradford reagent and measuring the amount of light absorbed by the protein. Use the plot below to determine the mass of protein (in micrograms, μμg ) in 100.0 mL of the solution if its absorbance is 0.398. A. 52 B. 3.1 C. 2.4 D. 0.52 E. 0.031How would a decrease in the wavelength of light affect the resolution power of a bright-field microscope?A sample is dissolved in water and analyzed using spectrophotometry. The sample shows an absorbance peak at a wavelength of 486 nm. Which one of the following adjustments would increase the absorption at that wavelength? O Diluting the sample with additional water. O Evaporating some of the water from the sample. Decreasing the path length, b. the light takes through the sample. Re-measuring the absorbance of the blank (water).
- True or false? A. Phosphorescence occurs at higher energy then fluorescence. B. Fluorescence occurs at longer wavelengths than phosphorescence. C. Fluorescence occurs at shorter wavenumbers than phosphorescence.You are required to measure a sample that absorbs at a wavelength of 300 nm and 4 µm.What would you use as your light source? Justify your answer.3. Discuss the relationship of refractive index to molecular polarizability.
- 1. Write a report comparing the principles, limitations/capabilities of the following microscopic techniques. (a) Optical Microscopy(b) Scanning Electron Microscopy(c) Transmission Electron Microscopy(d) Atomic Force Microscopya) The refractive index (μ) of water is found to have the values 12.9,12.5,12.3 and 11.8.Calculate the standard deviation for the refractive index of water.The refractive index of water is 0.33 .a 1.33 .b O 1.5 .c O 0.5 .d O
- Nanoparticle was synthesis from silver atoms with 1.8 nm of diameter. Find the ratio of the surface atoms of this particle.what are the applications of Atomic Force Microscopy and explain eachCite one research article that used an IR spectrophotometer in their study and discuss how they used the instrument to generate their data and how they used the data of IR in answering their hypothesis.