1) Draw a chromatogram depicting separation of proteins 1, 2, and 3(protein #1, mw 30,000, protein #2, mw 60,000, and protein #3, mw 90,000).b)Indicate on the chromatogram in (a) where insulin would elute. Would insulin have total accessibility, some accessibility or no accessibility to the particle pores on this column?c)Referring to the size exclusion chromatogram in (a), indicate on the chromatogram where you would expect a molecule with a size of 40,000 to elute. Would you expect the molecule to be well resolved, somewhat resolved, or not resolved from the peak for protein #1? TABLE 26-4 Representative molecular exclusion mediaGel filtration in open columnsTSKgel SW silica for HPLCFractionation range forFractionation range forglobular proteins (Da)5 000-150 00010 000-500 000Pore sizeNameglobular proteins (Da)Name(nm)Sephadex G-10Sephadex G-25Sephadex G-50Sephadex G-75Sephadex G-100Sephadex G-200to 7001 000-5 0001 500-30 000G2000SWG3000SW12.52545G4000SW20 000-7 000 0003 000-80 0004 000-150 0005 000-600 000NOTE: Sephadex is manufactured by GE Amersham Biosciences. TSKgel SW silica is manufactared by Tosoh Bioscience.

The diagram of chromatograph is as follows-

1) Draw a chromatogram depicting separation of proteins 1, 2, and 3 (protein #1, mw 30,000, protein #2, mw 60,000, and protein #3, mw 90,000). b)Indicate on the chromatogram in (a) where insulin would elute. Would insulin have total accessibility, some accessibility or no accessibility to the particle pores on this column? c)Referring to the size exclusion chromatogram in (a), indicate on the chromatogram where you would expect a molecule with a size of 40,000 to elute. Would you expect the molecule to be well resolved, somewhat resolved, or not resolved from the peak for protein #1?

1) Draw a chromatogram depicting separation of proteins 1, 2, and 3 (protein #1, mw 30,000, protein #2, mw 60,000, and protein #3, mw 90,000). b)Indicate on the chromatogram in (a) where insulin would elute. Would insulin have total accessibility, some accessibility or no accessibility to the particle pores on this column? c)Referring to the size exclusion chromatogram in (a), indicate on the chromatogram where you would expect a molecule with a size of 40,000 to elute. Would you expect the molecule to be well resolved, somewhat resolved, or not resolved from the peak for protein #1?

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter5: The Eukaryotes

Section: Chapter Questions

Problem 1UTM

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