2. Joey was tasked to ensure that an enzyme responsible for keeping his species from extinction remains biologically active. To do so, he needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component Ka Conjugate Base Salt KYA Benzoic acid, C6H5COOH 6.25 x 10-5 NaC6H5COO MUH Boric acid, H3BO3 5.81 x 107 NaH₂BO3 TWO Hydrogen cyanide, HCN 6.20 X 10-10 NaCN A. In what buffer system (KYA, MUH, or TWO) will Joey store the enzyme? In your solution sheet, show calculations and briefly explain to support your answer. B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet C. Calculate the amount (in mL) of the 1.75 M stock solution (pH 7.00) of the same buffer system needed to prepare 250 mL of a 0.550 M buffer pH 7.00.

Chemistry: Principles and Practice
3rd Edition
ISBN:9780534420123
Author:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Publisher:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Chapter16: Reactions Between Acids And Bases
Section: Chapter Questions
Problem 16.7QE
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10.2 answer correctly and clearly or I will leave negative remarks. 

2. Joey was tasked to ensure that an enzyme responsible for keeping his species from extinction remains
biologically active. To do so, he needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only
three buffer systems are available in their laboratory:
Buffer System Weak Acid Component
Ka
Conjugate Base Salt
KYA
Benzoic acid, C6H5COOH 6.25 x 10-5 NaC6H5COO
MUH
Boric acid, H3BO3
5.81 x 107 NaH₂BO3
TWO
Hydrogen cyanide, HCN 6.20 X 10-10 NaCN
A. In what buffer system (KYA, MUH, or TWO) will Joey store the enzyme?
In your solution sheet, show calculations and briefly explain to support your answer.
B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet
C. Calculate the amount (in mL) of the 1.75 M stock solution (pH 7.00) of the same buffer system needed to
prepare 250 mL of a 0.550 M buffer pH 7.00.
Transcribed Image Text:2. Joey was tasked to ensure that an enzyme responsible for keeping his species from extinction remains biologically active. To do so, he needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component Ka Conjugate Base Salt KYA Benzoic acid, C6H5COOH 6.25 x 10-5 NaC6H5COO MUH Boric acid, H3BO3 5.81 x 107 NaH₂BO3 TWO Hydrogen cyanide, HCN 6.20 X 10-10 NaCN A. In what buffer system (KYA, MUH, or TWO) will Joey store the enzyme? In your solution sheet, show calculations and briefly explain to support your answer. B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet C. Calculate the amount (in mL) of the 1.75 M stock solution (pH 7.00) of the same buffer system needed to prepare 250 mL of a 0.550 M buffer pH 7.00.
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