2-5 pls PART 2: INITIATION OF REPLICATIONQUA T2 ANOBelow is a figure representing a time lapse of DNA replication of a eukaryoticchromosome. This is the same chromosome, shown every 15 min after the process ofDNA synthesis has begun. The original DNA is black and the newly made strands areshown in grey.The asterisk represents the origin of replication. At the origin, the two strands of thehelix are pulled apart.*1.Draw arrows into the replicationbubbles to show the direction ofmovement of the replicationforks.Time Lapse5.ک ہیun to a hold onible2. What types of bonds areoccurring at the origin betweenthe enzyme and the DNAbackbone (what functionalgroups are in the backbone of grine od ni amoleDNA?)?LIGHTHOE5'in in5'*5. Below is a chromosome mid-replication. TAK*odgorli lens(1603. What types of bonds are occurring at the origin between the enzyme and theDNA nitrogenous bases?4. As the DNA strands are separated, what bonds are being broken?eroitonut isnW OIsnotonutter Wmina. Place an asterisk at each origin of replication above.b. Why do you think eukaryotic chromosomes use multiple origins ofreplication?348

Eukaryotic DNA replication is a tightly controlled process involving numerous enzymes and proteins.…

2607 A47) eblosun to alcold prihlucker P * woled oiterarion 2. What types of bonds are occurring at the origin between the enzyme and the DNA backbone (what functional groups are in the backbone of prise cillni amote no DNA?)? Time is i 5' 5. Below is a chromosome mid-replication. wong ents 3. What types of bonds are occurring at the origin between the enzyme and the DNA nitrogenous bases? 4. As the DNA strands are separated, what bonds are being broken? QUOT L And to frienggmoo a. Place an asterisk at each origin of replication above. b. Why do you think eukaryotic chromosomes use multiple origins of replication? 35

2607 A47) eblosun to alcold prihlucker P * woled oiterarion 2. What types of bonds are occurring at the origin between the enzyme and the DNA backbone (what functional groups are in the backbone of prise cillni amote no DNA?)? Time is i 5' 5. Below is a chromosome mid-replication. wong ents 3. What types of bonds are occurring at the origin between the enzyme and the DNA nitrogenous bases? 4. As the DNA strands are separated, what bonds are being broken? QUOT L And to frienggmoo a. Place an asterisk at each origin of replication above. b. Why do you think eukaryotic chromosomes use multiple origins of replication? 35

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter14: Dna Structure And Function

Section: Chapter Questions

Problem 17RQ: Which of the following is not a true statement comparing prokaryotic and eukaryotic DNA replication?...

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Figure 14.14 You isolate a cell strain in which the joining of Okazaki fragments is impaired and suspect that a mutation has occurred in an enzyme found at the replication fork. Which enzyme is most likely to be mutated?
5' 3' For numbers 6 to 10, refer to the image above and answer the questions. 6. Which among the two strands will have a continuous replication? 7. Which is the lagging strand? 8. Along which strand will Okazaki fragments appear? 9. How are Okazaki fragments joined together? 10. Where should the 3' end of the lagging strand be located? On the right or left side? in
PART 4: TERMINATION OF REPLICATION When the ends of linear chromosomes are reached there is a 3' overhang of single stranded DNA. This is because the RNA primer located at this position has been removed and DNA polymerase cannot replace that primer with DNA (without a primer). This apparent dilemma is solved by an enzyme called Telomerase. Telomerase extends the DNA at the end of chromosomes, called telomeres. Telomeres are stretches of repeated And yd 5 ITT 3¹ 5 TTTTT 3¹ LL 3' is in 35mming 5' Telomere Repeats TITTAGGG TTAGGG TTAGGG 3 5' CCAAUCCC of chromosomes. Telomerase 3 Telomere Repeats 5⁰ VITTAGGGTTAGGG TTAGGG TTAGGG 3 AATCCC AATCCC AAUG( CCAAUCCC 9.0 Telomerase 1. The RNA nucleotides part of the telomerase structure is used as the In ins DNA polymerase DNA sequences. Telomerase recognizes the repeated sequence, binds to it, and then lays down more nucleotides, lengthening the telomere. 3' Telomerase is composed of amino acids and RNA nucleotides. In the image above the amino…
AKS 5b: Which statement is correct regarding the semiconservative nature of DNA? * The semiconservative nature of DNA allows for genetic stability in somatic gene production MRNA operates as a template to allow DNA to replicate itself using ribosomes The structure of the phosphate group on the DNA molecule direct the correct nucleotides into place during replication Nucleotides in each original strand serve as a template for the new strand to be made AKS 5b: Which model accurately represents the semi-conservative nature of DNA replication? * * AA AA AA AA АВ ВА AA BB AA AA АВ АС Figure A Figure B Figure C Figure D
Below is a picture of a single origin of replication in a eukaryotic cell. 5' 3' 5' 1. On the figure above, Draw out where the following molecules will be located: Helicase; Sliding Clamp, Single Strand Binding Protein. 2. On the right hand side of the dotted line, the replication of which template strand (top or bottom) will be continuous by DNA polymerase? 3. On the left hand side of the dotted line, the complete replication of which template strand (top or bottom) will be more affected by a mutation that causes DNA ligase to be partially functional?
PLEASE HELP ME WITH THIS ONEMatch the statement to the corresponding agent/key player in DNA replication. Some items require more than one answer. Choices: Origin of replication Bubble SSBP RPA Sliding Clamp PCNA DNA Pol III Pol ε Pol δ DNA Pol I RNAse H Flap 1 DNA gyrase Pol α DNA helicase Primase Single chromosome Multiple points in chromosomes DNA ligase Not applicable Holds the processive enzyme in prokaryotes Start of replication in prokaryotes Holds the processive enzyme in eukaryotes Primosome in prokaryotes Dissociates after adding the few initial nucleotides in eukaryotes Adds more nucleotides in the lagging strand of prokaryotes Make primers for the replicative enzymes in eukaryotes Adds more nucleotides in the leading strand of prokaryotes Adds more nucleotides in the leading strand of eukaryotes Adds more nucleotides in the lagging strand of eukaryotes Start of replication in eukaryotes Removal of eukaryotic primer Breaks the H-bonds of bases Proof reading in prokaryotic…
DNA Replication Topoisomerase LAstand Primase E strand D Match the diagram with the correct term listed below. Structures Letter Okazaki fragments DNA polymerase RNA primer Helicase Leading strand Lagging strand 3' 5'
A. Which of the followings are characteristics related to eukaryotic replication? * 1. DNA linear in nature 2. Replication takes place in cytoplasm 3. DNA circular in nature 4. DNA replicates in nucleus 5. One replication origin 6. Unwinds in multiple areas
to 4 minutes) The schematic diagram below shows organization of the DNA replication fork. Match parts of the diagram (labeled A-F) with the corresponding term from the answer list (designated 31 parental duplex 5' 3' fork progression v A 1Lagging strand 2. An Okazaki tragment 3.Site of action of DNA topoisomerase 4 Leading strand 5. Site of action of DNA helicCase 6.Site of action of DNA ligase
.5' 3' For numbers 6 to 10, refer to the image above and answer the questions. 6. Which among the two strands will have a continuous replication? 7. Which is the lagging strand? 8. Along which strand will Okazaki fragments appear?
AKS 5c1: The process represented in the model involves the creation of a molecule that is complementary to the template molecule. According to the model, what type of molecule is being created? * Nontemplate strand Polymerase Ribonucleotide Template strand New DNA Polypeptide O Messenger RNA Carbohydrate V 0 4:57 Sign out ET O O O O
DNA Replication Drawing Name: Using penci, you will draw a representation of DNA replication along the leading and lagging strands. Follow the directions below, drawing each element in its proper location along the replicating DNA strand. Once you are sure everything is in the correct place, complete your drawing by adding color to distinguish objects as separate. 1. On the diagram below, label the 5 and 3' onds of both parental DNA strands (you can make up which is which) 2 Label the replication fork 3. Draw and label helicase 4. Label the overall direction of DNA replication 5. Draw and label single stranded binding proteins 6. Draw and label the leadng strand 7. Draw and label a single DNA polymerase IIl on the leading strand 8. Draw and label an RNA primer on the leading strand 9. Draw and label a DNA polymerase I on the leading strand 10. On the lagging strand, draw and label at least three Okazaki fragments 11. On the lagging strand. draw and label at least two DNA polymerase IIl…