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3. A mixture of proteins shown in the table below are dissolved in water and injected into a size exclusion chromatography system. The column is rated to separate molecular weights from 10,000 to 90,000. Given the calibration curve for the SEC column below and the data in the table showing the mixture of proteins injected, draw a qualitative curve to show the expected results (chromatogram) of running the SEC by showing detection units (RI or UV absorbance) on the y-axis and time on the y-axis. Relative peak heights (areas under curves) and times should be as quantitative as possible. ID Protein Molecular Weight Conc pl Туре 1 Collagen 68,000 8 g/L 7.8 2 Lactoperoxidase 92,600 1 g/L 6.5 345678 Insulin 5,800 12 g/L 7.3 fibrous α-helices; structural antibacterial enzyme globular protein Fibronectin 55,000 5 g/L 4.6 fibrous protein Lysozyme 16,000 2 g/L 4.6 cell-lysing enzyme Trypsin 47,000 10 g/L 2.5 digestive enzyme Cytochrome C 12,400 1 g/L 9.6 mitochondrial protein Immunoglobulin G 150,000 2 g/L 7.3 antibody in blood Calibration Curve for SEC Column Note: as shown in the calibration curve, the beads in the column can separate molecules less than ~90,000 molecular weight, but any proteins larger than that will elute at, matching the time for 90,000 MW proteins to eluse (~8 minutes). The minimum molecular weight that can be separated in the column is 10,000; smaller proteins elute together at a time matching 10,000 MW (~31 minutes). The equation of the calibration curve is shown on the graph. log(Molecular Weight) 7 6.5 6 5.5 log10(MW) 5.266 -0.0396 t 5 4.5 4 3.5 3 2.5 04 8 12 16 20 24 28 32 36 Retention Time (min)

3. A mixture of proteins shown in the table below are dissolved in water and injected into a size exclusion chromatography system. The column is rated to separate molecular weights from 10,000 to 90,000. Given the calibration curve for the SEC column below and the data in the table showing the mixture of proteins injected, draw a qualitative curve to show the expected results (chromatogram) of running the SEC by showing detection units (RI or UV absorbance) on the y-axis and time on the y-axis. Relative peak heights (areas under curves) and times should be as quantitative as possible. ID Protein Molecular Weight Conc pl Туре 1 Collagen 68,000 8 g/L 7.8 2 Lactoperoxidase 92,600 1 g/L 6.5 345678 Insulin 5,800 12 g/L 7.3 fibrous α-helices; structural antibacterial enzyme globular protein Fibronectin 55,000 5 g/L 4.6 fibrous protein Lysozyme 16,000 2 g/L 4.6 cell-lysing enzyme Trypsin 47,000 10 g/L 2.5 digestive enzyme Cytochrome C 12,400 1 g/L 9.6 mitochondrial protein Immunoglobulin G 150,000 2 g/L 7.3 antibody in blood Calibration Curve for SEC Column Note: as shown in the calibration curve, the beads in the column can separate molecules less than ~90,000 molecular weight, but any proteins larger than that will elute at, matching the time for 90,000 MW proteins to eluse (~8 minutes). The minimum molecular weight that can be separated in the column is 10,000; smaller proteins elute together at a time matching 10,000 MW (~31 minutes). The equation of the calibration curve is shown on the graph. log(Molecular Weight) 7 6.5 6 5.5 log10(MW) 5.266 -0.0396 t 5 4.5 4 3.5 3 2.5 04 8 12 16 20 24 28 32 36 Retention Time (min)

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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3. A mixture of proteins shown in the table below are dissolved in water and injected into a size exclusion chromatography system. The column is rated to separate molecular weights from 10,000 to 90,000. Given the calibration curve for the SEC column below and the data in the table showing the mixture of proteins injected, draw a qualitative curve to show the expected results (chromatogram) of running the SEC by showing detection units (RI or UV absorbance) on the y-axis and time on the y-axis. Relative peak heights (areas under curves) and times should be as quantitative as possible. ID Protein Molecular Weight Conc pl Туре 1 Collagen 68,000 8 g/L 7.8 2 Lactoperoxidase 92,600 1 g/L 6.5 345678 Insulin 5,800 12 g/L 7.3 fibrous α-helices; structural antibacterial enzyme globular protein Fibronectin 55,000 5 g/L 4.6 fibrous protein Lysozyme 16,000 2 g/L 4.6 cell-lysing enzyme Trypsin 47,000 10 g/L 2.5 digestive enzyme Cytochrome C 12,400 1 g/L 9.6 mitochondrial protein Immunoglobulin G 150,000 2 g/L 7.3 antibody in blood Calibration Curve for SEC Column Note: as shown in the calibration curve, the beads in the column can separate molecules less than ~90,000 molecular weight, but any proteins larger than that will elute at, matching the time for 90,000 MW proteins to eluse (~8 minutes). The minimum molecular weight that can be separated in the column is 10,000; smaller proteins elute together at a time matching 10,000 MW (~31 minutes). The equation of the calibration curve is shown on the graph. log(Molecular Weight) 7 6.5 6 5.5 log10(MW) 5.266 -0.0396 t 5 4.5 4 3.5 3 2.5 04 8 12 16 20 24 28 32 36 Retention Time (min)
Transcribed Image Text:3. A mixture of proteins shown in the table below are dissolved in water and injected into a size exclusion chromatography system. The column is rated to separate molecular weights from 10,000 to 90,000. Given the calibration curve for the SEC column below and the data in the table showing the mixture of proteins injected, draw a qualitative curve to show the expected results (chromatogram) of running the SEC by showing detection units (RI or UV absorbance) on the y-axis and time on the y-axis. Relative peak heights (areas under curves) and times should be as quantitative as possible. ID Protein Molecular Weight Conc pl Туре 1 Collagen 68,000 8 g/L 7.8 2 Lactoperoxidase 92,600 1 g/L 6.5 345678 Insulin 5,800 12 g/L 7.3 fibrous α-helices; structural antibacterial enzyme globular protein Fibronectin 55,000 5 g/L 4.6 fibrous protein Lysozyme 16,000 2 g/L 4.6 cell-lysing enzyme Trypsin 47,000 10 g/L 2.5 digestive enzyme Cytochrome C 12,400 1 g/L 9.6 mitochondrial protein Immunoglobulin G 150,000 2 g/L 7.3 antibody in blood Calibration Curve for SEC Column Note: as shown in the calibration curve, the beads in the column can separate molecules less than ~90,000 molecular weight, but any proteins larger than that will elute at, matching the time for 90,000 MW proteins to eluse (~8 minutes). The minimum molecular weight that can be separated in the column is 10,000; smaller proteins elute together at a time matching 10,000 MW (~31 minutes). The equation of the calibration curve is shown on the graph. log(Molecular Weight) 7 6.5 6 5.5 log10(MW) 5.266 -0.0396 t 5 4.5 4 3.5 3 2.5 04 8 12 16 20 24 28 32 36 Retention Time (min)
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