Assignment_12.pdf - Adobe ReaderFile Edit View Window HelpОpen1/ 199,1%Tools Fill & Sign Comment3. Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cellgrowth. The recombinant protein can be considered a product of cell culture even though it is notsecreted from the cells; it is synthesized in addition to normal E. coli biomass. Ammonia is used asthe nitrogen source for aerobic respiration of glucose.The recombinant protein has an overall formula of CH1.5500.31N..25. The yield of biomass (excludingrecombinant protein) from glucose is measured as o.48 g/g; the yield of recombinant protein fromglucose is about 20% of that for cells.How much ammonia is required?What is the oxygen demand?If the biomass yield remains at 0.48 g /g, how much different are the ammonia and oxygenrequirements for wild-type E. coli that is unable to synthesize recombinant protein?а.b.с.24°C08:39Berawan27/04/2022

Disulfide-bonded proteins primarily mature in the oxidative environment of the eukaryotic…

Answered: 3. Recombinant protein is produced by a…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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וןווד ← Q Lab Report 6 worksheets 314 F22 .DOCX File Edit View Insert Format Tools Help A 100% ¿ Summary Grades for Arysta Visser: 23 x M Uh-oh! There's a problem w X b The restriction EcoRI cleaves X + Untitled spreadsheet - Goog X https://docs.google.com/document/d/1mKY1HIgMPRh1kRDCmX7msBF2yf07-ogT/edit Outline Headings you add to the document will appear here. Normal text Times ... 12 + B I U 2 18. The restriction EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3', the restriction enzyme HindIII cleaves at the sequence 5'-AAGCTT-3', and the restriction enzyme BamHI cleaves at 5'GGATCC-3. An 805 bp circular plasmid is digested with each enzyme individually and then in combination, and the resulting fragment sizes are determined by means of electrophoresis. The results are as follows: 1 Restriction Enzyme(s) EcoRI BamHI HindIII EcoRI and BamHI EcoRI and HindIII BamHI and HindIII 3 Practice ====•=•€ EX Fragment lengths (base pairs) 430 bp, 375 bp 470 bp, 335 bp Lab Report 6…
I need help writting a biological "title" for this lab report. I performed 5 labs which are PCR purification, Rapid ligation, and Transformation, Blue/White screening and observation of pGreen transformation, Plasmid Extraction and Restriction Enzyme Digestion, Soil DNA extraction and gel electrophoresis, and Bioinformatics Analysis of 16s rRNA genes. Note; I attached one page of my abstract and one page of the introduction
patibility Mode] UE Layout References Mailings Review View A A Aa vA 前、一间刷一| T AaBbCcDdE AaBE x A Av X, Normal No Spacing Headi co Edit and Save Start your free one month trial of Microsoft 365 or sign in to activate an existing subscription. 9. In human beings, the gene for red-green colorblindness (r) is sex-linked and recessive to its allele for normal vision (R), while the gene for freckles (F) is autosomal and dominant over its allele for nonfreckled (f). A nonfreckled, normal-visioned woman whose father was freckled and colorblind, marries a freckled, colorblind man whose mother was nonfreckled. a. What is the genotype of the woman's father? b. What is the probability that the couple's first child will be a non-freckled, normal visioned girl c. What is the probability that the first two children born to the couple will be freckled and colorblind girls? d. What is the probability that the first child born to the couple will either be a freckled, colorblind boy or a non-freckled,…
AutoSave O Off # 2 Mol Bio Mutation QUESTIONS - Saved to this Pc - O Search A MS T MCHUNU MT File Home Insert Design Layout References Mailings Review View Help Chemistry A Share P Comments (i SIGN IN TO OFFICE It looks like your stored credentials are out of date. Please sign in as 20*****@ke****** za so we can verify your subscription. Sign In Task B [5 points]: Sanger sequencing Having successfully amplified the NRAS gene from wild-type and cancerous tissue through PCR, you now sent your PCR product for sequencing. However, the only available technology to you is classical Sanger sequencing or the dideoxy chain termination method, explained in Figure 2. Shown below are the sequencing gels obtained for the sections of the wild-type and mutant NRAS genes where the mutation can be found. WILD-TYPE NRAS MUTANT NRAS ddA ddT ddC ddG ddA ddT ddC ddG 1. Determine the 5'-to-3' sequence of the wild-type TEMPLATE strand. Answer: 5' 3' 2. Using the codon table given below, determine the…
ule_2023_Spring_Stu x M Inbox (1) - Ic21-0506@lclari X =6104_EP#6 - Google Docs ent/d/104sNODGApfrUg768nDS9piPmOB-VzXs86b_t-tDcDF8/edit My Drive - Google... WebAdvisor Main... ols Extensions Zotero Help text T Arial Haplontic life cycle Diplontic life cycle Parthenogenesis Karyotype Histone - 11 + 2 4. Match the words with the correct definitions: | Alternation of generations Job Listing - USF... WT Enrollment Requir... B I U 3 4 X 0 Lecture Video: The Cell Cyc x Chemical Principl... UO UND Orn Z = - 1:3 A type of reproduction that produces a clone of the mother A protein DNA wraps around it to condense the DNA An organism alternates between 1n and 2n with each generation A complete set of chromosomes, as they appear in the nucleus When an organism spends most of its life in the 1n state When an organism spends most of its life in the 2n state 11
Search (Option + Q) rences Review View Help Editing v A^ Dv A A A Question 1 During nucleic acid hybridization, the probe is labelled for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification Question 2 10 IV 12 13 14 Which of the following best describes the trait in the pedigree? Question 2 options: X-linked dominant X-linked recessive autosomal domiant autosomal recessive
the image is the steps for help. Not writing assignment!!!! What is the intended outcome of the lysozyme-based ion exchange chromatography described above? Will the protein be effectively separated using the materials listed in the image, or will it fail? Give a brief explanation for your decision.
personal/eenongen_my_tnstate_edu/_layouts/15/doc.aspx?sourcedoc={a6b083c9-a226-4c31... O Search (Option + Q) Review View Help Editing v A BI U Ov =<=v 三 v ... Question 13 Zinc finger nuclease is a Gene editing tool: which uses a site-specific endonucleases containing a DNA-cleavage domain and a modular DNA-binding domain RNA-guided endonucleases programmable nucleases Exonuclease Question 14 Which elements are required for DNA to behave as a chromosome in yeast cells: a centromere an autonomous replicating sequence two telomeres Antibiotic selection marker Question 15 A series of such clones where the insert of each clone partially overlaps that of its neighbors with no gaps is known as a Clone Contig DNA fingerprinting A tiling path Genome MacBook Pro
available to you). Summarize the results of your research on table 1.4. The first item is given as an example. Table 1.4: Transgenic Organisms and How They Benefit Human Society Field How the Organism Benefits Human Society (Limit your explanation to 2 or 3 sentences.) Because of these transgenic E. coli, human insulin can producing E. coli now be obtained without using human pancreas from cadavers. This also made possible the production of large amounts of human insulin over a short period of time. Allergic reactions from insulin injections are also Transgenic Organism Human insulin- Medicine prevented. Medicine
9:33 AM Fri Jun 11 @ 1 100% The_Farmer_and_the_Fly Home Insert Draw Layout Review View Times New Roma I U A.. A READ ONLY - This is an older file format. To make changes, make a copy of the file in the new format. Upgrade a copy THE FARMER AND THE FLY: PART I Directions: Read each part of the scenario Determine a response for the problem which correlates to part of the scenario you have just read. Scenario part I. A farmer was working with chickens at an agricultural research station. The population of flies in the chicken house was so large that the health of the chickens was affected. So the farmer decided to spray the chicken house and the chickens with a solution of insecticide, Formula A. The insecticide worked well and killed nearly all of the flies. Sometime later, however, the number of flies was again large. The farmer sprayed the chicken house and the chickens with insecticide, Formula A again. Many of the flies were killed, but not all. Unfortunately, after a short time, the…
UPVOTE WILL BE GIVEN! ANSWER IN 3-5 PARAGRAPHS (TYPEWRITTEN) a. What are the applications of modern biotechnology in the field of engineering? b. What are the implications of these applications? c. How do you think these applications will change the world in the future?
Copy and paste the link below and watch the video in Youtube and Answer the Questionshttps://www.youtube.com/watch?v=8RBs0Ghg_48 Gel electrophoresis Questions 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?

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