5. The following kinetic data are recorded for equal amounts of the enzyme, proline racemase,in the absence and presence of an inhibitor, I. Based on the Lineweaver-Burk plots:a. What type of inhibitor is I?b. Calculate the Km and Vmax in the presence and absence of the inhibitor?c. Calculate Ki of the inhibitor when 0.4mM inhibitor is used.0.50.40.30.20.1-0.2-0.10.10.20.30.4-0.11/[S] (1/mM)1/V (min/mM)

Competitive inhibitors bind to the free enzyme and show changes in the slope of the plot whereas…

5. The following kinetic data are recorded for equal amounts of the enzyme, proline racemase in the absence and presence of an inhibitor, I. Based on the Lineweaver-Burk plots: a. What type of inhibitor is 1? b. Calculate the Km and Vmax in the presence and absence of the inhibitor? c. Calculate Ki of the inhibitor when 0.4mM inhibitor is used. 0.5 0.4 0.3 0.2 30.1 -0.2 -0.1 -0.1 0.1 0.2 0.3 0.4 1/[S] (1/mM) 1/V (min/mM)

5. The following kinetic data are recorded for equal amounts of the enzyme, proline racemase in the absence and presence of an inhibitor, I. Based on the Lineweaver-Burk plots: a. What type of inhibitor is 1? b. Calculate the Km and Vmax in the presence and absence of the inhibitor? c. Calculate Ki of the inhibitor when 0.4mM inhibitor is used. 0.5 0.4 0.3 0.2 30.1 -0.2 -0.1 -0.1 0.1 0.2 0.3 0.4 1/[S] (1/mM) 1/V (min/mM)

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Help blic Health Ch x 100% 47 HSC 258 - Major Projec X MindTap - Cengage Lea x C The Illustration To- 55750828934189288909969212&elSBN=9781305657571&id%3D1061392007&nbld%321... Q Search References Use the References to access important values if needed for this question. For the following reaction, 65.6 grams of barium hydroxide are allowed to react with 40.9 grams of sulfuric acid. barium hydroxide (aq) + sulfuric acid (aq) >barium sulfate (s) + water (1) What is the maximum amount of barium sulfate that can be formed? grams What is the FORMULA for the limiting reagent? grams What amount of the excess reagent remains after the reaction is complete? Submit Answer