5.. A sample of cells has a total receptor concentration of 25 mM. Ninety percent of thereceptors have bound ligand and the concentration of free ligand is 125 µM. Calculate the Kfor the receptor ligand interaction. (a) What is the AG° for binding for this interaction at 25°C?

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Answered: 5.. A sample of cells has a total…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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(b) What is the binding site' of a receptor? How does the binding site change shape during activation? ہے۔
17. Explain the concepts of specificity, competition, and saturation as they relate to membrane receptors. Explain how membrane receptors show specificity, competition, saturation. Some key concepts which relate to this question are first messengers, binding sites, and ligands.
A ligand binds two different receptors with a Kd value of 10−7 M for receptor 1 and a Kd value of 10−9 M for receptor 2. For which receptor does the ligand show the greater affinity? Calculate the fraction of receptors that have a bound ligand ([RL]/RT) in the case of receptor 1 and receptor 2 if the con- centration of free ligand is 10−8 M.
6. For a 1:1 ligand-receptor binding interaction that is modeled by X + Y XY : (A) Show how the fraction of ligand-receptor binding (fo) is represented by the [X] [X]+ K, equation of f, (i.e., derive this expression for this reaction beginning eg with the relationship for K = f([X].[X],[XY]) under equilibrium conditions). (B) shown to the right for this reaction, what is the estimated value of Keg? Give your answer in units of M. For the data plot 0.9 0.8 0.7 0.6 문 0.5 0.4 0.3 0.2 0.1 0.0000 0.0050 0.0100 0.0150 0.0200 0.0250 0.0300 0.0350 0.0400 [X) (mM)
19. Which of the following statements is FALSE? A) Nucleotides and amino acids can act as extracellular signal molecules. B) Some signal molecules can bind directly to intracellular proteins that bind DNA and regulate gene transcription. C) Some signal molecules are transmembrane proteins. D) Answer: Dissolved gases such as nitric oxide (NO) can act as signal molecules, but because they cannot interact with proteins they must act by affecting membrane lipids. Explanation:
From the Hill Plot below, the of the first binding event for the receptor-ligand system under study is: Q1 4nM Ο 10 μΜ -2 nM 2 nM Calculate the Hill Coefficient from the receptor-ligand binding data below: Q2 4 100 2 3 (0-1) 60 log 10 8 6 4 2 0 -2 -4 -6 -6 -4 -2 0 2 4 log [L] (nM) 6 8 10
2) After plotting actin bound to cofilin versus the cofilin concentration, you generate the figure below. v (cofilin binding density) 1 0.8 0.6 0.4 0.2 0 2 4 6 8 [S. pombe cofilin] free (UM) 10 a) What is the Kd you measure for this interaction? b) What is the AG of the interaction? c) Based on the data, is there anything you suspect about the binding of protein and ligand?
3) Acetylcholine binds to a GPCR on heart muscle, making the heart beat more slowly. The activated receptor stimulates a G protein, which opens a K+ channel in the plasma membrane, as shown in Figure 1. Which of the following would enhance this effect of the acetylcholine? (a) (b) (c) (d) Figure 1 addition of a high concentration of a non-hydrolyzable analog of GTP addition of a drug that prevents the a subunit from exchanging GDP for GTP mutations in the acetylcholine receptor that weaken the interaction between the receptor and acetylcholine mutations in the acetylcholine receptor that weaken the interaction between the receptor and the G protein
15. In order to function, all extracellular signal molecules must be transported by their receptor across the plasma membrane into the cytosol. A) True B) False Answer: Explanation:
Help me please
1) How many binding sites per molecule does the receptor have? 2) What type of cooperativity is observed with this binding protein? a) No cooperativety b) Positive homotropic cooperativity c) Positive heterotropic cooperativity d) Negative homotropic cooperativity e) Negative heterotropic cooperativity f) none of the above
2 of 16 Compound Binds to Elicits biological receptor response A Yes Yes B Yes No C No No Refer to the table above. Compound X is the natural ligand that binds to a receptor and stimulates a signal transduction pathway associated with the receptor. Based on the information given in the table, what are the roles of compounds A, B, and C? O A is an antagonist, B is an agonist, and C is a ligand. A is an agonist, B is an antagonist, and C has no role. A is a ligand, B has no role, and C is an antagonist. A is an agonist, B is an antagonist, and C is an agonist. A has no role, B is an agonist, and C is an antagonist.

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5.. A sample of cells has a total receptor concentration of 25 mM. Ninety percent of the receptors have bound ligand and the concentration of free ligand is 125 µM. Calculate the Kp for the receptor ligand interaction. (a) What is the AGº for binding for this interaction at 25°C?