6. An 80-year-old man patient was admitted to the hospital with a fever of 102 F. A sputum culture revealed many gram-negative rods on MacConkey agar and blood agar. The patient was diagnosed with pneumonia. The following biochemical results were obtained from the culture: H;S = Neg %3D Lactose = + Urease = + Citrate = + Indole = + VP = + Motility = Neg Resistance to ampicillin and carbenicillin %3D Name organism:
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- A culture of Staphylococcus of unknown density is diluted as follows (letters represent each step): • 60 mL of a bacterial culture is diluted by adding 440 mL of water • 1000 microliters from (A) is added to 9 mL of water • Dilution (B) is used to prepare a 103 dilution in water • 100 ul from dilution (C) is plated to nutrient agar and incubated at 37°C Following incubation, colonies are uniformly distributed on the agar, and 102 colonies are counted on one half of the plate. 1. Express the total CFU/mL of the original culture in scientific notation. 2. If 10 ul is plated from dilution (C), how many colonies should grow on the plate? Edit View Insert Format Tools Table1. The differential aspect of MacConkey agar allows for the determination of which bacteria are lactose fermenters and which are not. 2. Blood agar is used to determine an organism's ability for hemolysis. Blood agar is differential in this regard. 3. EMB agar uses two dyes, eosin and methylene blue, to restrict the growth of gram-positive organisms, making this agar (EMB) in this regard. 4. Crystal violet dye in MacConkey agar will prevent some organisms from growing, making MacConkey agar in this regard. The crystal violet dye can also be used in the determination of the ability to ferment the carbohydrate in the agar; making MacConkey agar in this regard. 5. Use of an agar which contains an elevated amount of salt would allow some organisms to grow while preventing the growth of other organisms. This media would be considered selectiveWhich bacterial culture (Lb. plantarum, P. acidilactici, or P. pentosaceus) was most effective against monocytogenes and Salmonella? Explain why. What are the limitations of using an agar disk diffusion assay to assess the effectiveness of an antiseptic, disinfectant, or, in this case, a biological control agent on the growth of bacteria of interest?
- A researcher is planning to plant antibiotic-resistant Escherichia coli pure bacteria sample in antibiotic (ampicillin) solid media in his experiment. Describe each of the sterilization methods that this researcher should use while preparing this solid medium with antibiotics and in cultivation of the bacteria on the solid medium, indicating the stages at which it should be used and giving the mechanism of action.1. Describe the three different type of hemolysis that are observed on blood agar.2. What is a selective medium?3. What is a differential medium?4. Which media can be used to isolate E. coli samples from contaminated lettuce?5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea?6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain.7. What is the color or TSI for Salmonella?8. What is a fastidious organism?15 - Which one is a disadvantage of UV emitters? Hangisi UV yayıcıların dezavantajıdır? A) minimum operation cost minimum işletme maliyeti B) No formation of disinfection by productsÜrünlerde dezenfeksiyon oluşmaması C) simplest ,homogeneity, low cost, reproducibility, controllability en basit, homojenlik, düşük maliyet, tekrarlanabilirlik, kontrol edilebilirlik D) it does not penetrate glass paper or plastic cam kağıda veya plastiğe nüfuz etmez E) max operational safety maksimum operasyonel güvenlik
- 3- Visible Growth in Tubes Growth in Petri Dishes 0 µg/ml 2 µg/ml 0 1 3 4 ÖÖÖÖÖÖÖÖ 2 5 6 7 number 1, and After addition of The figure given above summarizes an experiment carried out to test the efficacy of an antimicrobial substance. In the tube number 0, no antimicrobial substance was added into bacterial culture. 2 µg/ml of antimicrobial substance was added into tube the amount of antimicrobial was doubled for each consecutive tube. antimicrobial into tubes, tubes were incubated for overnight and after incubation tubes were observed for growth. The growth status of bacteria in each tube was shown as (+) and (-). In addition to observation of visible growth, 1 ml of sample from each tube was collected and plated into petri dishes and incubated for additional overnight. Similarly, the growth status in petri dishes was as (+) and (-). According to given information; a. Determine the minimum inhibitory concentration (MIC). b. Determine the minimum bactericidal concentration (MBC). c.…1. To test the ability of each diluent to sustain the viability of Escherichia coli during dilution, the cells were incubated at room temperature (20 to 23°C) or 37°C for 0 or 2 h and plated on NA agarin duplicate. The counts obtained are shown in the following table: E. coli counts on dilution time O hour 2 hours Diluents 10-5 10-6 10-7 10-5 10-6 10-7 R1 26 ; 27 3:2 0:0 0:0 0:0 0:0 Distilled Water R2 31 ; 27 1:0 0:0 0:0 0:0 0:0 (DW) R3 27 : 28 4:5 0:0 0:0 0:0 0:0 R1 290 : 295 34 :31 2:1 252 : 239 27 : 26 1;1 DW-0.85% NacI R2 315 ; 309 37 : 34 2;2 250 : 245 26 ; 26 1:0 R3 301 ; 285 36 : 44 1:2 235 ; 267 34 : 21 0:0 R1 385 ; 392 42 : 35 4:5 368 :379 33 ; 32 2:2 DW-3.0% Nacı R2 370 : 388 37 : 40 2:4 305 : 303 35 ; 30 2:5 R3 355 : 365 35 : 28 4:6 370 :350 33: 37 3:1 R1 380 ; 383 30 : 27 2:2 255 : 245 25 : 24 2;2 PBS R2 341 ; 352 27 : 34 1:3 230 : 245 26 : 27 2:2 R3 329 ; 335 33 : 31 3:6 242 : 250 28 : 29 2:1 R1 340 ; 320 32 : 35 1:0 245 : 249 27 : 25 1 1 PBS-0.85% Naci R2 340 ; 345 35 :…In lab we learned a technique that helped us to visulize individual colonies of bacter 1. Describe this technique. 2. What do you expect the resutls to look like? Be specific. 3. How can this technique help you to determine if your culture is contaminated? For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). BIUS Paragraph I +] F H Ix X ABC † ( O K₂ KN Q V Arial sè "Ω Θ A 4 10pt EE 88 A Click Save and Submit to save and submit. Click Save All Answers to save all answers. 描く前 X² X₂ 3 由用目
- A pure culture of an unknown bacterium was streaked onto plates of a variety of media. You notice that the colony morphology is strikingly different on plates of minimal media with glucose compared to that seen on trypticase soy agar plates. How can you explain these differences in colony morphology?a.Describe what makes thioglycollate medium suitable for culturing anaerobes. What would the growth patterns of Clostridium sporogenes and Micrococcus luteus be in this medium? b. In the Kligler test, why do we inoculate the surface of the agar slope and then stab into the butt of the slope? What does a pink coloured colony indicate when using MAC (MacConkey Agar)?Why do Salmonella growing on XLT-4 agar is red colony with black center. Explain it in detail and concisely.