8) Explain why DNA with a higher ratio of A-T base pairs compared to G-C base pairing would have a lower melting point.

Chemistry: Matter and Change
1st Edition
ISBN:9780078746376
Author:Dinah Zike, Laurel Dingrando, Nicholas Hainen, Cheryl Wistrom
Publisher:Dinah Zike, Laurel Dingrando, Nicholas Hainen, Cheryl Wistrom
Chapter23: The Chemistry Of Life
Section: Chapter Questions
Problem 65A
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7) You encounter a bunch of cells with glucose concentration inside of 0.10 mM and they are suspended in a
solution that has a glucose concentration of 0.010 mM.
ww
a) Will glucose travel into or out of the cells?
b) What is the free energy change in kl/mol for the transport of glucose from the medium into the cell? Assume a
temperature of 37 °C.
8) Explain why DNA with a higher ratio of A-T base pairs compared to G-C base pairing would have a lower
melting point.
9) Ethidium bromide is a compound commonly used to visualize DNA during gel electrophoresis, though its use
has been phased out due to toxicity and mutagenic concerns. This compound stains DNA by intercalating. Explain
what this means in the context of DNA base pairs.
Transcribed Image Text:7) You encounter a bunch of cells with glucose concentration inside of 0.10 mM and they are suspended in a solution that has a glucose concentration of 0.010 mM. ww a) Will glucose travel into or out of the cells? b) What is the free energy change in kl/mol for the transport of glucose from the medium into the cell? Assume a temperature of 37 °C. 8) Explain why DNA with a higher ratio of A-T base pairs compared to G-C base pairing would have a lower melting point. 9) Ethidium bromide is a compound commonly used to visualize DNA during gel electrophoresis, though its use has been phased out due to toxicity and mutagenic concerns. This compound stains DNA by intercalating. Explain what this means in the context of DNA base pairs.
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