9. Recombinant DNA could be transformed into a cell through various methods such as: Transformation method Description Ligated product incubated with competent E. coli, heat-shock treatment. Cells incubated with the plasmid DNA are subjected to a high-voltage pulse. Transiently renders the bacterial membrane permeable to large molecules. The process of injection of foreign DNA into a cell using micropipette/micromanipulator.

9. Recombinant DNA could be transformed into a cell through various methods such as: Transformation method Description Ligated product incubated with competent E. coli, heat-shock treatment. Cells incubated with the plasmid DNA are subjected to a high-voltage pulse. Transiently renders the bacterial membrane permeable to large molecules. The process of injection of foreign DNA into a cell using micropipette/micromanipulator.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter8: The Structure, Replication, And Chromosomal Organization Of Dna

Section: Chapter Questions

Problem 3QP

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The figure below illustrates the stages of production of useful medical products using recombinant DNA technology. Closely study the figure then answer the Activity 1 questions that follow: Human cell Bacterium 1. DNA Plasmid DNA Human insulin-producing gone 2. DNA is cut with restriction Bacterial DNA with human gone inserted enzymes. 3. Plasmid is reintroduced into bacterium. 4. Engineered bacteria multiply producing insulin. 5. Insulin is separated and purified to produce human insulin. Human insulin Itulin 6. Insulin injected into patient 1. What is a plasmid? Make a diagram of bacterial cell containing a plasmid. 2. How do you explain the selection of plasmids for carrying the desired gener 3. Follow the steps of human insulin production, then 4. Make a diagram for the production of growth hormone. first
Enzymes of bacterial origin used in a wide variety of techniques are: ligases restriction endonucleases primase dna polymerase resctriction exonuclease
Categorize the following as being associated with transformation, conjugation, or transduction. Note that some items may be associated with more than one process. Transformation Conjugation Transduction requires a virus can transfer dead bacterium's DNA to a new host cell efficient way to get naked plasmid into E. coli cells in the lab involves a pilus describes type of horizontal gene transfer in bacteria cells need to be competent process exploitod by biotechnology transfer requires at least two living bacteria alters bacterial genome
Biology Question
Question Image hown bele nallest? Q. A gel from gel electrophoresis is shown below. Which DNA fragment is the smallest? B. Direction of Travel
Which of the following would be the most likely consequence for a bacterial colony where the pLZDonorGuide was properly transformed into the bacteria but the arabinose was not added to the LB plate Group of answer choices The bacteria will have a white color The bacteria will have a blue color Homology directed repair (HDR) will be activated The bacteria will die
During which step in the transformation protocol does the plasmid DNA move into the cell? Heat Shock Grow in LB media at 37°C Plate on media with Ampicillin O Incubate the cells on ice with CaCl2 in the absence of plasmid. fg f1o 19 %24 & 5 6. 7 8. 4 5 R Y U P F G J 2 K 3] vの

Drag and drop the appropriate text in the gaps below. Firstly, the plasmid DNA in the E. coli is propagated through Then the bacterial cells are pelleted by centrifugation at 10,000 RPM. The media supernatant is removed and the bacterial cells are The bacterial cells are then lysed via |. Contaminating macromolecules such as protein and chromosomal DNA is removed by Overnight incubation of the bacterial culture washed in cell resuspension buffer addition of lysis buffer addition of neutralisation buffer washing of the spin column separation via agarose gel electrophoresis
For which of the following would not require a nucleic acid probe?a. locating a gene on a chromosomeb. developing a Southern blotc. identifying a microorganism with FISHd. constructing a recombinant plasmid
Which of the following protocols is BEST used for introducing exogenous DNA into a cell with a thick outer barrier (e.g. plant cell wall)? electroporation O heat-shock transformation gene gun Olipofection
Which of the following is NOT true about Base Excision repair? Group of answer choices Ligase is responsible for resealing the nick in the phosphodiester backbone. Endonuclease is used to remove the damaged nucleotide. It is a preferred method of removal for larger defects in the genetic code Polymerase creates the new nucleotide to replace the damaged one Glycosylase removes damaged the damaged base before the nucleotide
The presence of colonies on the bacterial plate post transformation suggests which of the following? Group of answer choices The colonies contain the transformed plasmid and are not resistant to the antibiotic The colonies contain the transformed plasmid and are resistant to the antibiotic The colonies do not contain the transformed plasmid and are not resistant to the antibiotic The colonies do not contain the transformed plasmid and are resistant to the antibiotic