________________ "cut" DNA at specific sequences and the resulting segment with sticky ends is "pasted" into a plasmid by ___________ to then be replicated in the host. Group of answer choices 1. Restriction enzymes; ethidium bromide 2 DNA polymerases; ligase 3 Restriction enzymes; ligase 4 Ligases; restriction enzymes

______ "cut" DNA at specific sequences and the resulting segment with sticky ends is "pasted" into a plasmid by _ to then be replicated in the host. Group of answer choices 1. Restriction enzymes; ethidium bromide 2 DNA polymerases; ligase 3 Restriction enzymes; ligase 4 Ligases; restriction enzymes

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 1SQ

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Similar questions

A ____________ is required to transfer DNA sequences from one organism to another. reporter gene vector genetic probe restriction endonuclease genomic library
Choose the letter that corresponds to the step in the process where a restriction enzyme is used.
Based on the image below, answer the following questions: Restriction enzymes PstI and ScaI were employed and the gel image after restriction digest is shown below. The name of the plasmid is Blank 1. The gel lane on the Blank 2 was digested using PstI enzyme. (left or right) The gel lane on the Blank 3 was digested using PstI and ScaI enzymes. (left or right)
PLEASE CHECK THE WORDS THAT ARE IN BOLD. THEY ARE THE SAME QUESTIONS BUT THE WORDS IN BOLD ARE DIFFERENT. Assuming a linear piece of DNA (plasmid) was used as starting material, how many restriction sites were there considering you observed three bands after agarose gel electrophoresis. Assuming a circular piece of DNA (plasmid) was used as starting material, how many restriction sites were there considering you observed two bands after agarose gel electrophoresis.
Use the sequence provided and make use of figure 1 to determine what restriction enzyme uses the spesific recognition site and figure 2 and 3 to detremine how many times does the sequence occur in the λ DNA sequence? GGATCC: __________________________________________________________ GAATTC: ___________________________________________________________ AAGCTT: ___________________________________________________________
The following image will be used to answer questions 1 through 9. Below is a restriction map of the circular plasmid YIP5. This plasmid contains 5,541 base pairs. There is an EcoRI site at base pair 1. The locations of other restriction sites are shown on the map. The numbers after the enzyme names tell at which base pair that enzyme cleaves the DNA. If you digest YIP5 with any one of the listed restriction enzymes you will end up with a linearized piece of DNA that is 5,541 base pairs long. EcoRI 1/5541 HindIII 32 Pvul 4916 Eagl 942 YIP 5 Apal 2035 Pvull 3247 Smal 2540 Reaction B) You digest YIP5 with two enzymes, Hindll and Apal, at the same time. How many DNA fragments would you expect from this reaction and what are the sizes of the fragments?
The following image will be used to answer questions 1 through 9. Below is a restriction map of the circular plasmid YIP5. This plasmid contains 5,541 base pairs. There is an EcoRI site at base pair 1. The locations of other restriction sites are shown on the map. The numbers after the enzyme names tell at which base pair that enzyme cleaves the DNA. If you digest YIP5 with any one of the listed restriction enzymes you will end up with a linearized piece of DNA that is 5,541 base pairs long. EcoRI 1/5541 HindIII 32 Pvul 4916. Eagl 942 YIP 5 Apal 2035 Pvull 3247 Smal 2540 Reaction A) You digest YIP5 with two enzymes, EcoRI and Eagl, at the same time. How many DNA fragments would you expect from this reaction and what are the sizes of the fragments?
Which statement is NOT the function of restriction enzyme? Group of answer choices It cuts the vector’s plasmid and the desired gene from the original DNA. It ligates the desired to gene to vector’s plasmid. It acts as scissors of DNA that cut along a specific sequence. It serves as degrading proteins that cut plasmid on specific target site.
U have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions. PscI & GsuI ______________________________________________________________ ScaI, PdmI & BsaXI ______________________________________________________________ ScaI, SspI & EheI ______________________________________________________________
Define the following terms: Plasmid Restriction Enzyme Standard Curve
which sample shows the plasmid is fully digested
Restriction Enzymes with LongerRecognition Sites Produce Larger _______ Fragments