A carboxypeptidase is a metalloenyme (its active site contains one or more metal ions essential for the function) that catalyzes the hydrolysis of the peptide bond of the terminal amino acid of a polypeptide chain (where the free carboxyl group occurs). The binding of an L-alanyl-L-tyrosine peptide substrate in the active site of the enzyme is represented in the scheme below: Glu ++ Zn -COO™ H3C OH CH₂ HC NH C C H O Poche apolaire C O +N; H H H -HO H C NH₂ H Arg 145 Tyr 248 NB: This scheme gives a planar representation of the spatial structure of the active site where indicated contacts (hatched lines) are supposed to occur in the 3D structure of the enzyme. 1- Describe the interactions that occur between the ligand and amino acid residues of the active site. 2- What would be the impact on the Km value if we replace L-alanyl-L-tyrosine by the following substrates: L-alanyl-L-phenylalanine; L-alanyl-L-aspartate; L-aspartyl-L- tyrosine.
A carboxypeptidase is a metalloenyme (its active site contains one or more metal ions essential for the function) that catalyzes the hydrolysis of the peptide bond of the terminal amino acid of a polypeptide chain (where the free carboxyl group occurs). The binding of an L-alanyl-L-tyrosine peptide substrate in the active site of the enzyme is represented in the scheme below: Glu ++ Zn -COO™ H3C OH CH₂ HC NH C C H O Poche apolaire C O +N; H H H -HO H C NH₂ H Arg 145 Tyr 248 NB: This scheme gives a planar representation of the spatial structure of the active site where indicated contacts (hatched lines) are supposed to occur in the 3D structure of the enzyme. 1- Describe the interactions that occur between the ligand and amino acid residues of the active site. 2- What would be the impact on the Km value if we replace L-alanyl-L-tyrosine by the following substrates: L-alanyl-L-phenylalanine; L-alanyl-L-aspartate; L-aspartyl-L- tyrosine.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
![A carboxypeptidase is a metalloenyme (its active site contains one or more metal
ions essential for the function) that catalyzes the hydrolysis of the peptide bond of the
terminal amino acid of a polypeptide chain (where the free carboxyl group occurs).
The binding of an L-alanyl-L-tyrosine peptide substrate in the active site of the
enzyme is represented in the scheme below:
Glu
Zn++
COO™
OH
H3C
CH₂
HC
NH
IO C
H
H
Poche apolaire
-H
H
O+N: C N
H
H
H
NH₂
Arg 145
Туг 248
NB: This scheme gives a planar representation of the spatial structure of the active
site where indicated contacts (hatched lines) are supposed to occur in the 3D
structure of the enzyme.
1- Describe the interactions that occur between the ligand and amino acid residues
of the active site.
2- What would be the impact on the Km value if we replace L-alanyl-L-tyrosine by the
following substrates: L-alanyl-L-phenylalanine; L-alanyl-L-aspartate; L-aspartyl-L-
tyrosine.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff1cc82da-1a95-413d-800c-76a0d9bb28c9%2Ff0c9c81a-fef9-428d-89f6-f3b4e2684b8e%2F5w3xgb_processed.jpeg&w=3840&q=75)
Transcribed Image Text:A carboxypeptidase is a metalloenyme (its active site contains one or more metal
ions essential for the function) that catalyzes the hydrolysis of the peptide bond of the
terminal amino acid of a polypeptide chain (where the free carboxyl group occurs).
The binding of an L-alanyl-L-tyrosine peptide substrate in the active site of the
enzyme is represented in the scheme below:
Glu
Zn++
COO™
OH
H3C
CH₂
HC
NH
IO C
H
H
Poche apolaire
-H
H
O+N: C N
H
H
H
NH₂
Arg 145
Туг 248
NB: This scheme gives a planar representation of the spatial structure of the active
site where indicated contacts (hatched lines) are supposed to occur in the 3D
structure of the enzyme.
1- Describe the interactions that occur between the ligand and amino acid residues
of the active site.
2- What would be the impact on the Km value if we replace L-alanyl-L-tyrosine by the
following substrates: L-alanyl-L-phenylalanine; L-alanyl-L-aspartate; L-aspartyl-L-
tyrosine.
Expert Solution
![](/static/compass_v2/shared-icons/check-mark.png)
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 3 steps with 1 images
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
Recommended textbooks for you
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781319114671/9781319114671_smallCoverImage.jpg)
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
![Lehninger Principles of Biochemistry](https://www.bartleby.com/isbn_cover_images/9781464126116/9781464126116_smallCoverImage.gif)
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
![Fundamentals of Biochemistry: Life at the Molecul…](https://www.bartleby.com/isbn_cover_images/9781118918401/9781118918401_smallCoverImage.gif)
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781319114671/9781319114671_smallCoverImage.jpg)
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
![Lehninger Principles of Biochemistry](https://www.bartleby.com/isbn_cover_images/9781464126116/9781464126116_smallCoverImage.gif)
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
![Fundamentals of Biochemistry: Life at the Molecul…](https://www.bartleby.com/isbn_cover_images/9781118918401/9781118918401_smallCoverImage.gif)
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781305961135/9781305961135_smallCoverImage.gif)
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781305577206/9781305577206_smallCoverImage.gif)
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
![Fundamentals of General, Organic, and Biological …](https://www.bartleby.com/isbn_cover_images/9780134015187/9780134015187_smallCoverImage.gif)
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON