A chromatogram of a mixture of species A, B, and C provided the following data: a) Define "Retention time". b) Calculate the resolution between species A and B. c) Calculate the retention time of species B necessary to separate it from species A with a resolution of 1.5.

A chromatogram of a mixture of species A, B, and C provided the following data: a) Define "Retention time". b) Calculate the resolution between species A and B. c) Calculate the retention time of species B necessary to separate it from species A with a resolution of 1.5.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter19: Nuclear Magnetic Resonance Spectroscopy

Section: Chapter Questions

Problem 19.12QAP

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Concept explainers

Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.

Question

A chromatogram of a mixture of species A, B, and C provided the following data:

a) Define "Retention time".

b) Calculate the resolution between species A and B.

c) Calculate the retention time of species B necessary to separate it from species A with a resolution of 1.5.

Retention time, min
Width of peak (W), min
Unretained
3.1
A
11.1
0.98
B
12.4
1.03
C
13.2
1.06

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