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- d) Since we know this bacterial organism thrives in colder areas of the world, what can be said about the effect of temperature on the growth patterns ofthis bacterial organism? How would increasing the temperature affect the growth constant? e) If the bacterial organism is resistant to antibiotics, how would administering antibiotics affect the growth of this organism?A batch of turkey rolls (10 lb—approximately 4.5 Kg—each) were cooked to 165°F internal temperature in bags, opened, sliced, vacuum-packaged, and stored at 40°F. The product was expected to have a refrigerated shelf life of 50 days. However, after 40 days, the packages contained gas and approximately 107 bacterial cells/g of meat. The bacterial species involved in the spoilage was found to be Leuconostoc carnosum, which is killed at 165°F. What could be the sources of the bacterial species in this cooked product?In Figure 5-3, if the concentration of bacterial cells inthe original suspension is 200/ml and 0.2 ml is platedonto each of 100 petri dishes, what is the expected average number of colonies per plate?
- Using a dilution series is a way to transfer the very small volumes of original culture necessary to get countable colony forming units. If you suspect your culture of bacteria has 59.62*10^9 cells per mL, how many mL of original culture would you want to end up on the plate to produce 68 cfu? Express your answer in scientific notation rounded to two decimal places. Eg. 0.01001 would be 1.00*10^-2 You must include trailing zeros 0.0000040001 would be reported as 4.00*10^-6 There must always be two decimal places, even if they are zeros Canvas expects one digit before the decimal point 0.40*10^-5 would be marked incorrect for 0.0000040001A species of bacteria has a doubling time of 24 hours. After incubating the initial population for one week, the final population was 6,400 cells. What was the initial population of that bacteria? Show all work. Equations: Nt = No x 2n Final concentration = (initial concentration)/(total dilution factor) Total dilution factor = (individual dilution factor)# of serial dilutionsA bacterial strain is growing exponentially. At 1:00pm the titre of the culture is 2x10^3 cells per ml. Three hours later, the culture is still growing exponentially and the titre is 4x10^4 cells per ml. How many generations have elapsed, what is the generation time, and what will the titre be at 9:00pm
- A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/L4. (a) Which of the following chemical reactions is an overall oxidation-reduction (redox) reaction that involves oxidation number changes in their reactions? A. NaOH + H₂O → Na¹ + H3O+ + OH B. HCl + H₂O → H3O+ + Cl- C. CO₂ + H₂O → H+ + CO3²- D. N₂ + 3H₂ → 2NH3A microbiologist inoculates a growth medium with 100 bacterial cells/ml. If the generation time of the species is 1 hour, and the microbes are in the exponential (log) phase of growth, how many hours require for the culture to surpass 10,000 cells/ml, if the culture is checked once each hour? O 1) 2 hours O 2) 7 hours O 3) 3 hours O 4) 10 hours O 5) 24 hours
- An inoculum of 106 bacterial cells was introduced into a flask of culture medium and growth monitored. No change was seen for 18 minutes (the lag phase), then growth occurred rapidly. After a further 87 minutes, the population had increased to 5.08 × 107 cells. What is the doubling time of the culture? Show your solution. (The 87-minute period is after the lag phase.)Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration.The solution containing bacterial spores is heated in an autoclave. When the autoclave has reached a constant temperature of 121 °C, there are still 106 spores/ml. The specific death constant for bacterial spores at 121 ° C is 11.62 min-1. How long should the heating be continued at a constant temperature so that there are 1 spores/ml left? Enter the answer in minutes to three decimal places.