A gel filtration column was calibrated by measuring elution volumes for two proteins of known mass: glutamate dehydrogenase, 290 kDa with elution volume 20.8 mL; serum albumin, 37 kDa with elution volume 42.5 mL Use these values to determine the equation of the straight line graph that relates log molar mass to elution volume.  The elution volume of an unknown protein was 32.2 mL.  Use the equation just determined above to estimate the molar mass of the protein in kDa units. Don't round your answer until the end to ensure your answer is within the accepted range.  Round your answer to the nearest whole number (e.g. If you calculated 27.52, input your answer as 28).

Q: Fractional saturation, Y, is represented by Y = PO₂" 2 n n pO₂+ P50' The term po₂ is the partial…

A: By the equation,                                              Y=pO2n/P50n+pO2n​values:P50=26 torr…

Q: A receptor-ligand complex has a dissociation constant of Kd = 20 nM. The rate of receptor-ligand…

A: The dissociation constant (Kd) for a receptor-ligand complex is related to the rate constants for…

Q: Which of the following functions describes the enzyme Peptidase? A. remove phosphate from a…

A: All living things depend on proteins in one way or another. The building components of peptides and…

Q: The following reaction is catalyzed by this type of enzyme? COO™ COO™ HIC-H HIC-H COO™ succinate…

A: The conversion of succinate to fumarate is an important step in the citric acid cycle (also known as…

Q: 0.5 M NaOH. Write the acid base reaction that is taking place and use an ICE table to show how the…

A: Number of moles in 100mL of 5M acetic acid can be calculated by the formulaVolume in L ×…

Q: Using the pka data for lysine, draw the molecular species present at each of the following pH values…

A: Lysine has 3 ionizable groups in it. They are;alpha- carboxyl groupalpha-amino groupside chain basic…

Q: Which foods are better at slowing down the rate of alochol absorption into the bloodstream?.

A: When alcohol is ingested, it is not digested and metabolised by our body. Instead, it enters the…

Q: Devise a solid-phase synthesis of the Ala-Leu-Val-Phe-Met, using Fmoc  protection and the…

A: Solid phase, chemical synthesis of peptides, takes place from the C-terminal to the N-terminal side…

Q: Would the base pair Guanosine and Cytosine be more or less stable at pH 10

A: The stability of a base pair like Guanosine (G) and Cytosine (C) can be influenced by pH, but in the…

Q: PCR is a molecular biology technique where template DNA is amplified using a primer and…

A: Polymerase chain reaction (PCR) is a molecular technique that is used to isolate as well as amplify…

Q: Some microbes like E. Coli have flexible metabolic systems that allow them to generate energy under…

A: E.coli can undertake both aerobic and anaerobic respiration to generate ATP (energy). ATP is…

Q: Each peak on the tandem mass spectrogram represents a product ion with a +1 charge. Use the…

A: Mass spectrometry is an analytical gel-free proteomics method used for identification of proteins,…

Q: Draw the Ala-Ser-Leu-Asp polypeptide (perspective formula) showing the fully extended backbone…

A: Proteins are folded peptides. Peptides are polymers made up of amino acid residues linked via a…

Q: Explain why unfolding or aggrrgation may occur using changes in entropy and enthalpy of the protein…

A: Unfolding or aggregation of proteins can occur due to changes in entropy and enthalpy, which are…

Q: How many peptide bonds are present in the peptide shown below?

A: By removing a water molecule from an amino group of one amino acid and a carboxyl group of the…

Q: Calculate the unknown concentration of the PROTEIN C with an absorbance value of A412 given the…

A: A linear regression analysis will be required to determine the unknown concentration of Protein C.…

Q: When C-2 is labeled, why does the label survive two full turns of the cycle but then become equally…

A: Oxaloacetate is a four-carbon compound, and carbon-2 (C-2) refers to the second carbon atom in the…

Q: =) D) CH₂ H₂N CH₂ CH₂ NH3 H3NCH-Coo CH₂ OH -CH-COO CH-OH CH3 H3NCH-COo CH-CH3 C10 NH₂

A: There are 20 naturally occurring amino acids in nature. Out of these 10 are essential and 10 are non…

Q: 7. Which of the following statements is true about enzyme-catalyzed reactions? a The reaction is…

A: The reaction  which occurs in the presence of an enzyme is called enzyme catalysed reaction.Enzymes…

Q: For the reaction A + B = C + D, assume that the standard change in free energy has a positive value.…

A: Consider the following general reaction:aA + bB ⇌ cC + dDAt equilibrium (steady state), the…

Q: The most abundant element, by mass, in living organisms is: a) C b)  O  c)  N  d)  Na Which of these…

A: Living organisms are primarily composed of water (H2O), which is the solvent within the body of a…

Q: An Fab fragment binds to lysozyme with a dissociation constant of Kd = 10-¹¹ M. A 1 nM (10—² M)…

A: To determine the concentration of added Fab at which half of the lysozyme will be bound to the Fab…

Q: 8. When oxygen binds to the heme group, by how many angstroms does the Fe2+ appear to shift? 9. How…

A: The heme group is a prosthetic group in oxygen-binding protein. Its primary function is to bind…

Q: In a sequence of amino acids, a non-polar amino acid is mutated into a polar charged amino acid.…

A: Protein or a polypeptide chain is made up of amino acids linked together by  peptide bonds. Protein…

Q: Use the polygon to draw the MO energy diagram for:

A: Here we can see the structure of Cyclotetraene with two lone pairs.The no. of pi electrons in…

Q: Estimate the apparent slope of the plot for myoglobin. myoglobin apparent slope = Estimate the…

A: The Hill plot is the graph we obtain by plotting   at X-axis and  at Y-axis. By looking at the value…

Q: Choose any/all that apply to protein synthesis. Ribosomes aid in the formation of peptide bonds…

A: As per the central dogma of molecular biology, genetic information for protein synthesis is stored…

Q: H CH₂ HỎ=CH. CH₂ H₂C (5) H CH₂ H₂C CH₂ CH₂ CH₂ ΝΗ C=NH NH₂ a) Which of the following statements…

A: Recall that:amino acid sequences are written with N-terminal amino acid on the left and C-terminal…

Q: Draw beta sheet with backbone hydrogen bonds, is this structure on the interior or exterior of the…

A: A typical structural motif in proteins is a beta sheet, commonly referred to as a beta strand. It is…

Q: Choose any/all that apply to the proton-motive force and ATP synthesis. The active pumping of…

A: The active pumping of protons through ATP synthase against their concentration gradient provides the…

Q: Addition of immunoglobulin G (IgG) specific for hemoglobin to a solution of hemoglobin results in…

A: Antibodies are proteins that are produced by the immune system to recognize and neutralize foreign…

Q: The amide hydrogen atoms of peptide bonds within proteins can exchange with protons in a solvent. In…

A: Hydrogen-deuterium exchange (HDX) is a technique that can be used to study the structure and…

Q: Proline provides structural regidity in proteins. What is the consequence of this on protein…

A: Of the 900 amino acids naturally occurring in nature, about 21 of them have been selected by…

Q: You dilute 10 µL of your isolated DNA with 90 µL of water. You then measure and record the…

A: To calculate the concentration of the plasmid DNA in μg/mL, we can use the following…

Q: Label the key components of a matrix-assisted laser desorption/ionization time-of-flight, or…

A: Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALD1-TOF) is a…

Q: 8. Which of the following fatty acids is most likely to be a liquid at room temperature? ОН -H OH OH…

A: Fatty acids are carboxylic acids with a hydrocarbon chain ranging from 4 to 36 carbons. In fatty…

Q: Some reaction components are shown on the left. Match them to the reactions catalyzed by glutamine…

A: Glutamine is a polar, uncharged amino acid. Glutamate is a negatively charged, acidic amino…

Q: This image shows the tertiary structure of a protein segment. Tertiary structure results from…

A: Ionic bond is formed between a positively charged and a negatively charged group. The charged groups…

Q: A water molecule has polar O-H bonds that result in regions of partial positive charge (hydrogen…

A: Ion dipole interaction is defined as the force of interaction between charged ions and polar…

Q: For a given reaction, CH3CHO+1/202 --> CH3COOH; the oxidizing agent is 02 O None of the above Осн…

A: The oxidizing agent is the species that is involved in reduction.The decrease in oxidation state is…

Q: 1. Which of the following best describes the relationship between a reactant molecule and an enzyme?…

A: Enzymes are biological catalysts made of proteins. Biochemical processes require enzyme catalyzed…

Q: Tyrosine came from the Greek word "tyros" which means cheese as it was discovered in cheese by…

A: Tyrosine is a crystalline amino acid with a molecular formula of C9H11NO3. It is a non-essential…

Q: Draw the structure of the peptide KNYPEH  showing every bond and atom (including the hydrogens) in…

A: Peptide- "KNYPEH"K- LysineN- AspargineY- TyrosineP- ProlineE- GlutamineH- Histidine

Q: Which of the following amino acids will bind to anion exchange resin at pH 7.0?

A: Anion-exchange chromatography is a type of ion exchange chromatography. Here the stationary phase…

Q: What characteristics determine the position of a protein on an IPG strip at the end of isoelectric…

A: Isoelectric focussing is a technique used for the separation of biomolecules, particularly proteins,…

Q: Please provide explanations and work for each part of the question. If able to keep the same format…

A: Protein structure is a fundamental concept within biochemistry, encompassing the hierarchical…

Q: Order the following cells in terms of the size of their receptive field, from smallest to largest:…

A: The size of receptive fields generally increases as you move from early sensory processing areas to…

Q: The compound below, doxorubicin, is a substrate for p-glycoprotein. Cancer cells with a higher…

A: Cancer is mainly caused by the uncontrolled proliferation of cells due to mutation. Doxorubicin is…

Q: In patients with Lesch Nyhan Syndrome, purine nucleotides are overproduced and over excreted. The…

A: The body creates purines through de novo synthesis. Hypoxanthine-guanine phosphoribosyltransferase…

Q: Arrange the steps of the formation of a secretory protein from its gene to its exocytosis. RNA…

A: Protein targeting is the process by which proteins are transported to their correct destinations…

Scientific Notation

We have to deal with quantities that are extremely large or very small. For example- I may have 6,500,000,000 red blood cells, or the diameter of an iron atom is 0.000000011 inches. Numbers with many zeros can be inexpedient to work with, so scientists use scientific notation. It represents very large numbers or very small numbers in a simple form. We know that whole numbers can be extended to infinity, but we cannot write such large numbers on paper. Numbers that present in millions of places after the decimal point need to be represented simply. Thus, it is difficult to represent a few numbers in their extended form. Therefore, we use scientific notation.