(a) Give a brief overview of chromatographic separation mechanisms. Your answer should include reference to adsorption, partition, ion exchange, gel filtration and affinity Indicate the mechanism most relevant to Gas Chromatography (GC). chromatography. (b) Using a drawing, describe in detail the structure of a typical column used for GC. Make sure to mention: (i) the dimensions and materials of the column itself and (ii) the chemical nature of any one common chromatographic stationary phase that could be mounted inside the column. (c) Explain why careful temperature control of the column is very important in GC and why the use of temperature programming can be advantageous.

(a) Give a brief overview of chromatographic separation mechanisms. Your answer should include reference to adsorption, partition, ion exchange, gel filtration and affinity Indicate the mechanism most relevant to Gas Chromatography (GC). chromatography. (b) Using a drawing, describe in detail the structure of a typical column used for GC. Make sure to mention: (i) the dimensions and materials of the column itself and (ii) the chemical nature of any one common chromatographic stationary phase that could be mounted inside the column. (c) Explain why careful temperature control of the column is very important in GC and why the use of temperature programming can be advantageous.

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter88: Column Chromatography

Section: Chapter Questions

Problem 3P

See similar textbooks

Similar questions

ANSWER ALL PLEASE: (a) Draw an outline schematic diagram of a typical instrument for High Performance Liquid Chromatography (HPLC) and label the individual components.(b) Discuss the materials used in the construction of the columns used in HPLC, giving the approximate dimensions of the column and the particle size of the typical column packing material.(c) Giving examples, discuss the chemical nature of the common stationary phases usedin HPLC making sure to distinguish between normal and reversed phase HPLC.
Justify following statements. (a) Paper chromatography is a type of partition chromatography. (b) The R f value is 1. (c) Chromatography is only a qualtitative technique. (d) The adsorbent should be insoluble in the solvent. (e) Chromatography is used to separate only color compounds.
Justify following statements. (a) Paper chromatography is a type of partition chromatography. (b) The R f value is 1. (c) Chromatography is only a qualtitative technique.
Provide a brief explanation of one specific application on High Performance Liquid Chromatography (HPLC). Incorporate the specific application name (e.g., Detection and Quantification of Mycotoxins), provide aconcise overview of sample preparation methods, outline instrumental parameters and conditions utilized, and summarize the outcomes and findings achieved through this analytical approach.
The partition coefficient of ammonia between water and chloroform is wanted to be determined. The necessary quantities of ingredients are taken into the separation funnel, and the extraction process is applied. At the end of the extraction process, 5 mL portions of each of the water and chloroform phases are titrated with 0.1 M HCI in the presence of methyl orange indicator. If the volumes spent at the turning points are 7.2 and 5.1 mL, respectively, calculate the KSU, KLOROFORM (Dispersion coefficient) value.
Justify and explain following statements. (a) The adsorbent should be insoluble in the solvent. (b) Chromatography is used to separate only color compounds.
Two commonly used chromatography methods in labs are Nickel and streptavidin resin based colums. Explain which type of chromatography-ion exchange, size-exclusion, or affinity-these are, as well as their seperation mechanisms at a molecular level.
(a) Write TWO (2) functions of XRF in materials characterisation.(b) Appraise the sample preparation for X-ray Diffraction (XRD) Test.(c) Support the idea of solid material can be described as amorphous and crystalline especially in XRD characterization.(d) Compare between X-ray Powder Diffaction (XRD) and X-ray Fluorescence Spectrometry (XRF) in terms of their similarities and differences.
(d) Explain why there may be a maximum operating temperature for the column and also why there has to be temperature control of the injector. (e) Flame lonisation (FI), Mass Spectrometric (MS) and Thermal Conductivity (TC) can all be used as detection techniques in GC. Discuss the advantages and disadvantages of each as a detection method. Which method is used in combustion analysis? (f) A solution of volume 1 mL, containing an equal parts mixture (by weight) of propofol and di-tertbutylphenol (a standard), was analysed by GC, which displayed peaks at retention times 5.80 min (area integration 2000) and 9.20 min (area integration 3500), respectively. An unknown solution, also volume 1 mL, was analysed under the same conditions and, among a number of other peaks, there was one at 5.80 min (area integration 4000). How could this be confirmed as propofol? In a subsequent analysis, 2.5 mg of di-tertbutylphenol was dissolved in the unknown solution and an extra peak appeared in the GC at…
(a) Which of the following parts in a gas chromatography (GC) system is/are heated up during operation – injector; column; detector? (b) Name ONE type of GC detector. Briefly comment on its advantage and limitation. (c) Why is chemical derivatization needed for GC?
Help me.... Explain the principles of gel permeation chromatography in Polymer, what is the application in Polymer analytics
Discuss the following effect on the retention time (increase, decrease, remain the same or become zero) of a peak in a liquid chromatography: Raising the column temperature, Decreasing the particle size of the column material (at constant pressure), Having bubble formed in the column, Increasing the column head pressure. (i) (ii) (iii) (iv)