Shown below are the maps of a series of rII− deletion strains (1–5). The deleted region is indicated as (......) and the intact region as ______.
1 ___________(...........)_______________
2 _________________(...........)_________
3 (.....................)_____________________
4 ________________________(................)
5 _____(..........)______________________
rII− phage strains A-E have point mutations in the rII region. E.coli K(λ) cells are coinfected with one phage that has a deletion and one phage that has a point mutation. The presence of wild-type progeny phage is assessed by the presence (+) or absence (o) of plaques.
|
|
1 |
2 |
3 |
4 |
5 |
|
A |
+ |
o |
+ |
+ |
+ |
|
B |
o |
+ |
+ |
+ |
+ |
|
C |
+ |
+ |
+ |
o |
+ |
|
D |
+ |
+ |
o |
+ |
+ |
|
E |
+ |
+ |
o |
+ |
o |
A) Indicate the order of the point mutations in the rII region.
- A) CADBE
- B) DEBAC
- C) BADCE
- D) ABDEC
- E) CEADB
B) The test described here is a recombination test. Explain Why?
⊚ true
⊚ false
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