A recombinant DNA contains and Which of the following terms is appropriate for the blanks? Lütfen birini seçin: O a. gene to be cloned / cell O b. plasmid / bacterial DNA O c. gene to be cloned/enzyme O d. plasmid / gene to be cloned

Q: The principle of the alkaline method for plasmid DNA isolation is: O A. An acidic solution…

A: Plasmid DNA isolation by alkaline lysis method This method is the most commonly used method to…

Q: Selectable markers allow Select an answer and submit. For keyboard navigation, use the up/down arrow…

A: A selectable marker is a gene that is added into a cell, specifically in a bacterium or to cells in…

Q: Define the following terms: a. biotechnology:…

A: DNA is a molecule discovered in the nucleus by Friedrich Meischer in the late 1860s, but its…

Q: In gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules…

A: Answer is b.)  have different lengths.

Q: which of the following are considered as the main tools for dna technology? a. vectors b. plasmids…

A: DNA technology is a technique which is used to create desired DNA molecule for enhancing the…

Q: A genetic sequence that can move from one location to an-other within a cell is known as a:(a)…

A: The genome comprises of the coding and non-coding sequences, in which the coding sequences are…

Q: Transfection efficiency refers to a) total number of transfected cells per dish O b) total number of…

A: Transfection is the process of introducing RNA or DNA intentionally in the eukaryotic cell…

Q: Plasmid vectors have the following characteristics, except _________ . a. MCS b. expression c.…

A: Introduction: A plasmid is a small, circular DNA molecule that is distinct from the chromosomal DNA…

Q: If the AMP was mistakenly left out of the LB/AMP/X-gal/IPTG agar media E. coli cells transformed…

A: *NOTE: Kindly repost for other question Dear Student as per the guidelines we are supposed to answer…

Q: A i. crossing over ii. biomarker a. Еcorl b. Recombinant iii. Gene-of-interest iv. restriction…

A: We know that crossing over is the major event during meiosis which leads to variations among…

Q: direction on the other strand. a. Palindromes b. snRNPs

A: Answer: a. Palindromes

Q: Which of the following statements is accurate for DNA replication in your cells, but not PCR? a.…

A: BASIC INFORMATION ABOUT GENETIC MATERIAL  DNA It stands for deoxyribo nucleic acid.  It is the…

Q: Which of the following is not required in order for a plasmid to be used in recombinant DNA…

A: A plasmid is a single-stranded, circular DNA molecule that is different from the chromosomal DNA of…

Q: 18. An instructor had her students perform this laboratory beginning with setting up their own…

A: Agarose gel electrophoresis is the technique for the separation of DNA fragments on the basis of…

Q: The difference between conjugative plasmids and mobilizable plasmids is that Select one: а.…

A: ANSWER;- d) None of the above Explain;- Conjugal plasmids encode a total arrangement of move genes…

Q: Variable number of tandem repeats (VNTRs) in the DNA molecule are highly useful in: A) Recombinant…

A: VNTRs are repeated units that occur in the genome and display length variations. The VNTRs consist…

Q: 3. A plasmid was digested with the enzymes BglII, Nsil and XhoI. A single digest was performed with…

A: A plasmid is an extrachromosomal small DNA molecule present in a cell and is also physically…

Q: 3000 bp 2000 bp 1000 bp 800 bp 700 bp 500 bp L Uncut EcoRI 1 BamHI Ncol EcoR1/BamH1 BamHI/Nco1…

A: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

Q: To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?a)…

A: The correct option is  CaCl2 (b) CaCl2 is the chemical which is added to prevent degradation of the…

Q: Why is Covid PCR testing also referred to as reverse transcriptase? O because it is done real-time O…

A: PCR (Polymerase Chain Reaction) is a technique, which is used to amplify a small fragment of DNA.…

Q: Choose the one answer that fits best.  Read carefully. Which of the following statements about the…

A: Option C is not correct-The plasmid used for transformation is called E.coli

Q: _____ cut(s) DNA molecules at specific sites. a. DNA polymerase c. Restriction enzymes b. DNA probes…

A: Recombinant DNA technology is the technique by which genes are combined from two different sources…

Q: is used to seal the phosphodiester backbones between vector and insert. O DNA polymerase O Ligase…

A: Phosphodiester bonds are formed when exactly two of the hydroxyl groups in the phosphoric acid react…

Q: Match the method with the appropriate enzyme.___ PCR                           a. Taq polymerase___…

A: Deoxyribonucleic acid is defined as a molecule that is composed of two polynucleotide chains that…

Q: A bacterium can naturally uptake DNA from environment. This is because, The bacterium contains…

A: The capacity of nucleic acids to guide their own reproduction from monomers makes them unique.…

Q: A. Use this data to generate a circular map of the plasmid. Pst I: 10 kb Bam HI: 10 kb Sal I:…

A: Restriction endonucleases are enzymes that cut DNA at specific sites. Restriction endonucleases…

Q: A bacterium can naturally uptake DNA from environment. This is because_ a. The bacterium contains…

A:

Q: he purpose of using a washing soap in DNA extraction experiment is to D a. Cut the proteins away…

A: DNA stands for deoxyribonucleic acid. It is present in the nucleus of the cell  It stores genetic…

Q: Which enzyme was used to produce the molecule in the figure above? O ligase O DNA polymerase O RNA…

A: DNA cloning, is a process in which a piece of gene is inserted into the circular piece of DNA…

Q: If the F plasmid does not have the gene encoding Fino, this F plasmid would Select one: a. become…

A: Fertility factor or F factor or F plasmid is a sex factor that helps to transfer the genes between…

Q: Q: Hyperglycosylated genetically engineered proteins synthesized by some micro-organisms are usually…

A: DNA: Deoxyribonucleic acid (DNA) is a polymer made of two polynucleotide chains that coil around one…

Q: Choose the combination of answers that most accurately completes the statement. Which of the…

A: Gene cloning is the process of employing genetic engineering techniques to create perfect clones of…

Q: Tissue growth factor-β (a) is a DNA probe for recombinant plasmids (b) is a product of DNA…

A: Introduction Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains that coil…

Q: During the restriction mapping of plasmid DNA, one control was used and it contained following. O a.…

A: Control DNAs are powerful tools in the investigation of genomic DNA in Maping procedures.and…

Q: You are studying a new plasmid, and you digest the plasmid with three restriction enzymes: Eco RI…

A: The plasmid map can be created by checking the individual band patterns and band patterns with a mix…

Q: You are studying a new plasmid, and you digest the plasmid with three restriction enzymes: Eco RI…

A: Introduction: Gel electrophoresis: The technique of separating DNA fragments as per their size is…

Q: Which of the following is/are not required for DNAreplication to occur?a. DNA polymerase d.…

A: DNA (short for deoxyribonucleic acid) is a double helical structure (with two DNA strands). A…

Q: write in one or two sentence for each please. Define the following terms: a. biotechnology:…

A: Introduction Biology:- It is a branch of science that deals with living organisms and their vital…

Q: High Frequency Recombination results in which of the following? O 1) Plasmid DNA incorporated into…

A: The bacterial DNA is arranged as a circular chromosome. The bacterial genome has a single origin of…

Q: When plasmids are used to produce a desired protein O the plasmids are inserted into the bacterial…

A: Plasmid are circular small double-stranded DNA molecules that exist independently of the host…

Q: Which of the following statements is false?a) The cells which take up recombinant plasmids are drug…

A: Plasmid is a circular DNA molecular that exists as an extrachromosomal molecule of DNA in a cell.…

Q: DNA fingerprinting uses to produce DNA fragments that can be separated and screened for the presence…

A: The DNA fingerprinting and human genome project both related to the field of molecular biology and…

Q: These highly polymorphic molecular markers are useful in DNA fingerprinting: (a) plasmid vectors (b)…

A: DNA typing is a laboratory procedure for detecting normal variations in a DNA sample…

Q: The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is dependent…

A: Agarose gel electrophoresis: It is used for the separation of DNA molecules that are larger than…

Q: What is the use of the filter in the plasmid extraction protocol?: Select one: O a. To get rid of…

A: The correct answer to the problem: The use of the filter in the plasmid extraction protocol is:d. To…

Q: irection: Encircle the letter of the best answer. What carries a gene from one organism into a…

A: DNA(deoxyribonucleic acid) is a double-stranded helical genetic material found in the nucleus of…

Q: FIGURE 2 shows the only suitable DNA restriction site in a plasmid DNA vector that can be cleaved.…

A: Restriction enzymes are the most important tool in genetic engineering. These enzymes have the…

Q: Recombinant DNA molecule is produced by ligatinga) Two DNA segmentsb) Two m-RNA moleculesc) One…

A: The method of producing many, identical copies of a single piece of DNA is DNA cloning. The DNA…

Q: Gene cloning vectors should have the property of self-replication so that DNA insert can be copied…

A: Cloning means to make copies in large amount of the cell which have the same genetic make i-e the…

Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.