A solution of DNA of unknown concentration (termed "[unknown] DNA solution") gave an absorbance value of 0.39 at 260 nm. The goal here is to determine the DNA concentration of this [unknown] DNA solution. To calculate this, you are given the following additional information: A DNA solution whose concentration is 50 ug/ml has an absorbance value of 1.0 at 260 nm. Using this information and knowing that A1/C1 = A2/C2 i. Calculate the DNA concentration (ug/mL) in the DNA solution of [unknown] ii. Once you have determined the DNA concentration of [unknown] DNA solution, calculate how many ug of DNA would be present in 25 μl of this DNA solution iii. iv. If you dilute the "[unknown] DNA solution" 10-fold, i.e. 1/10, what will the absorbance value (at 260 nm) of the diluted solution be? What will the new concentration of DNA of the diluted solution be?

A solution of DNA of unknown concentration (termed "[unknown] DNA solution") gave an absorbance value of 0.39 at 260 nm. The goal here is to determine the DNA concentration of this [unknown] DNA solution. To calculate this, you are given the following additional information: A DNA solution whose concentration is 50 ug/ml has an absorbance value of 1.0 at 260 nm. Using this information and knowing that A1/C1 = A2/C2 i. Calculate the DNA concentration (ug/mL) in the DNA solution of [unknown] ii. Once you have determined the DNA concentration of [unknown] DNA solution, calculate how many ug of DNA would be present in 25 μl of this DNA solution iii. iv. If you dilute the "[unknown] DNA solution" 10-fold, i.e. 1/10, what will the absorbance value (at 260 nm) of the diluted solution be? What will the new concentration of DNA of the diluted solution be?

Organic Chemistry

8th Edition

ISBN:9781305580350

Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Publisher:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Chapter20: Dienes, Conjugated Systems, And Pericyclic Reactions

Section: Chapter Questions

Problem 20.26P

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