(a) Will phenylalanine test positive or negative in the biuret test? Why? (b) What color do you expect when phenylalanine is added to the biuret test?
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- 4. a) The molecule below is a/n: amino acid dipeptide tripeptide (circle one) b) Would the molecule below test positive in the biuret test? Why/why not? If yes, label and point an arrow to the portion of the molecule responsible. H. H. NH3-C-C-NH-C-C CH3 CH2SHComplete the table below by adding (+) or (-) if eahc sample (amino acid or protein) below will test positive or negative, respectively. sample BIURET NINHYDRIN XANTHOPROTEIC MILLON-NASSE SAKAGUCHI LEAD ACETATE PAULY HOSKIN-COLE G W H Y C R HAIR GELATIN CASEIN PEPTONE Identify the amino acid that will give the following data: BIURET NINHYDRIN XANTHOPROTEIC MILLON-NASSE SAKAGUCHI LEAD ACETATE PAULY HOPSKIN-COLE - + + + - - + - Name of amino acid: structurte of the amino acid:1. Breaking of disulfide bonds in albumin to produce -SH is most likely caused by?* Acetic acid Alcohol Reducing agents Sun (ultraviolet radiation) 2. Positive with Molisch Test, but negative with both Iodine Test and Benedict's Test.* Glucose Fructose Ribose/Arabinose Maltose Sucrose 3. Which of the following is not a reducing sugar?* Sucrose Glucose Fructose Lactose
- Question 1: choose the proper structure of the predominant form of L-D-D-S-L-Q at pH 10. Question 2: choose the proper structure of the predominant form of D-I-L-W at pH 6.A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show workingOwing to their structural similarity, separation of mixtures of organic compounds (such as the isomers 1-t- butyl-, 3-t-butyl-, and 4-t-butylcyclohexene) often proves difficult. Silica gel chromatography, however, can be used with some success provided that sufficient silica gel is used to provide optimal separation. Consider, for example, the TLC plate pictured at right after developing and UV visualization where prior to elution, lanes A and B were spotted with 1-t-butylcyclohexene and lanes B and C spotted with 4-t- butylcyclohexene. Which of the following must be true? In the space provided, indicate the CORRECT answers (as 1, 2, and/or 3) then explain your reasoning for all three statements. 1. 1-t-butylcyclohexene elutes faster than 4-t-butylcyclohexene. 2. The R; for both 1-t-butylcyclohexene and 4-t-butylcyclohexene are equal. 3. 4-t-butylcyclohexene is less polar than 1-t-butylcyclohexene
- What are the colour changes occur to the reducing sugars after benedict test is performed? Please mention the colour change observed for each reducing sugars in benedict test2) Please draw a-mannose 1,4' B-talose.An octapeptide was hydrolyzed and the amino acids separated. Its amino acid composition was determined: Gly (1); Ala (2); Val (1); Lys (2); Met (1); Asp (1). After incubation with FDNB and hydrolysis, 2,4-dinitrophenylalanine was detected. NO2 H H N C COOH ON- CH, Treatment with cyanogen bromide, which cleaves at M, resulted in two fragments: a pentapeptide containing two K residues and a tripeptide containing V, G, and A. The residues are not listed in any particular order. After treatment with trypsin, which cleaves at R and K, there were three fragments: two dipeptides and MGAV. What is the sequence of the octapeptide? Use the one-letter abbreviations for the amino acids, and do not use dashes.
- Complete the table below with the results/colors obtained: Unhydrolyzed RNA Hydrolyzed RNA Tests performed Color (+) or ( - ) Color (+) or ( - ) 1. Benedict's 2. Orcinol 3. Purine bases 4. Inorganic phosphate * (+) = present (-) = absent %3DXanthoproteic test is used to detect amino acids containing an aromatic nucleus in a protein solution which gives yellow color, but why does phenylalanine gives negative result?Which of the following is an anomer of B-D-gulopyranose? O O го ОН H ОН H ОН H H ОН CH₂OH -II- H H ОН ОН CH2OH H ОН ОН CH2OH О 0. H CH2OH то Н H H ОН H H - о H ОН ОН H ОН H ОН ОН H О ОН ОН H N