a. The feed to the precipitation unit has 120 mg mAb/l and 28 mg impurity/l in 5 liters of product solution. Determine if 93% of the mAb can be recovered with a purity of at least 94% in the precipitation. State the concentration of each component in the solution, the mass of each component in the precipitate, volume of the solution and the mass of ammonium sulfate required. mAb solubility equation: ln S = -0.0252x + 10.2 impurity solubility equation: In S = -0.0153x +8.01 mg species I solution S = g/L ammonium sulfate b. The mAb and impurity have similar molecular weight and pl. Explain a purification step that can be used after precipitation to remove the final impurity. Describes the steps required in the process to purify the mAb.

a. The feed to the precipitation unit has 120 mg mAb/l and 28 mg impurity/l in 5 liters of product solution. Determine if 93% of the mAb can be recovered with a purity of at least 94% in the precipitation. State the concentration of each component in the solution, the mass of each component in the precipitate, volume of the solution and the mass of ammonium sulfate required. mAb solubility equation: ln S = -0.0252x + 10.2 impurity solubility equation: In S = -0.0153x +8.01 mg species I solution S = g/L ammonium sulfate b. The mAb and impurity have similar molecular weight and pl. Explain a purification step that can be used after precipitation to remove the final impurity. Describes the steps required in the process to purify the mAb.

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter5: The Eukaryotes

Section: Chapter Questions

Problem 2UTM: Sterile normal saline is used in most surgical procedures as an irrigation solution. During certain...

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