SBI 3C1VIRTUAL LAB: THE MICROSCOPEINSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow theinstructions on how to properly use a microscope. When you are complete, answer the questions below.PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen?TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification)Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPEОBJECTIVELENSPOWEROCULARMAGNIFICATIONOBJECTIVE LENSMAGNIFICATIONTOTAL MAGNIFICATIONMAG (X) = Ocular X ObjectiveLOWLP MAG =MEDIUMMP MAG=HIGHHP MAG-Complete the following chart by calculating the missing lens or total magnification [2]TOTAL MAGNIFICATIONOBJECTIVE LENS MAGNIRAR (EYEPIECE)MAGNIFICATION5X80X10X40X10X100X500X50XPART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDESAccess the Virtual Microscope at http://www.ncbionetwork.orglietimicroscopelGo to the "EXPLORE" tab. Click on the slide box → choose HUMAN → dlick on BLOOD to view the HUMAN BLOOD slide. You will needto use the coarse and fine focus and adjust the light to get clear views. The slide can also be moved within the viewing field.QUESTIONS:1. When you observed a prenared blood sample under low power, medium power and high power, what did you notice as you INCREASEDmagnification? Explain.2. What is the SHAPE of the blood cells?3. What organelles do you see under HIGH power?4. The cell contains many other organelles; why do you NOT see all of them through the microscope

Note: as you have asked multiple questions. We are instructed not to answer all the parts.…

Answered: AB: THE SBI 3C1 INSTRUCTIONS: Go to the…

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter33: Fluoroscopy

Section: Chapter Questions

Problem 1RQ

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Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.
Using a Light Microscope to Determine an Object's SIZE PRE-LAB QUESTIONS Fill in the diagram of the microscope with the term or description that matches, the microscope part. Eye Plece Body Tube Contains lens to increase magnification usually 10x Revolves to allow changing various objectives Arm Objectives Moves stage up and down approximately to correct distance Hold slides in place Stage Permits finer focusing by moving the stage in smaller increments Regulates the amount of light going through the stage Base Light Source Copyright © 2012 Laying the Foundation®, Ic., Dallas, Texas. All rights reserved. Visit us online at www.lftralning.org.
List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step. 1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage. a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage. 4. Use the Low Power Objective. a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…
A. Purpose: Figure 1 B. Materials: Microscope Magazine Slides and cover slips Paper towels Pipette Scissors C. Procedure: 1. Careful carry a microscope to your lab area. Make sure to hold it with one hand under the base and one hand on the arm as shown in Figure 1. 2. Plug the microscope in and turn it on. Take a moment to look at all the parts of the microscope. Then look at your ocular lens. What is the magnification of the ocular lens (eye piece)? Figure 2 3. Fill in the chart to show the total magnification for each objective lens. Magnification of Ocular Lens Magnification of Objective Lens Objective Lens Total Magnification Low Power Medium Power High Power
PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_ The field of view (FOV) is the circular area you can see when you look through the microscope. The diameter of the field of view is different depending on which objective lens you are using. For example, you are using the medium-power objective lens, then the area you can see is actually smaller than if you were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate actual size of objects / cells seen through the microscope. When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1). Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left edge of the field of view (see figure 2). NOTE: Slage cip I-1000 Objects in the FOV are usually measured in micrometers (um). To convert, a FOV in mm, times it by 1000…
Please include your reference below for my further research. Thank you! 1. What are the basic components of a Fluorescence Microscope and what are the functions of each? 2. Are there any parts that you can remove without compromising accuracy and utility of the equipment? 3. Can you suggest additional components to improve the equipment?
Write the term that the phrase describes. 1. Large knob that moves the stage or objective lens a great distance. Used with scanning or low-power objective lenses only. 2. Flat platform beneath the objective lens on which the microscope slide is placed. 3. Removable lenses that you look through to observe the microscope slide. 4. Small knob that moves the stage or objective lens a very small distance and is used for precision focusing. 5. Extends from the arm and contains the ocular lenses and rotating nosepiece. 6. Lens that condenses light through the specimen and is located below the stage. 7. Light from specimen passes through these lenses first. These lenses are located in the rotating nosepiece. 8. Wide bottom part that supports the microscope. 9. Regulates the amount of light passing through the condenser. 10. Vertical portion that connects the base to the head.
Match the terms on the left with its description _____ stage _____ocular lens ____Diaphragm _____adjustment focusing knob _____Body tube holds the microscope slide lens you look through, usually 10x power regulate the amount of light passing the specimen used to focus on specimen holds the eyepiece upper part of the microscope that contains optical components. holds the object lens magnifies specimen secures that slice to the stage condenses light from the light source into a narrow beam source of illumination magnifies an image 100x,
Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)
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Answer these questions by watching the YouTube videos and reviewing the Powerpoints from Lab #4. 1. What is Refraction of light? 2. What is the difference between the Ocular lens and the Objective lens? 3. What is the purpose of the Revolving Nosepiece? 4. What is the difference between the Course Adjustment Knob and the Fine Adjustment Knob? 5. How do you calculate Total Magnification? 6. What is Resolution in terms of Microscopy? 7. What is the purpose of Oil Immersion? 8. What is the Diffraction Barrier and why does it exist? 9. What is the purpose of using stains and fluorescent dyes in microscopy? 10. What is the advantage of using an Electron Microscope? 11. What Objective lens should you always start with? 12. What is the purpose of the Iris Diaphragm on the Condenser? 13. How do you know your Objective lens has been adjusted properly? 14. Why should you not use Kimwipes…
4) Answer true or false to each of the following statements. On high power, you should use the coarse adjustment knob. The diaphragm determines how much light shines on the specimen. The low power objective has a greater magnification than the scanning objective. The fine focus knob visibly moves the stage up and down. Images viewed in the microscope will appear upside down. If a slide is thick, only parts of the specimen may come into focus. The type of microscope you are using is a scanning microscope. For viewing, microscope slides should be placed on the objective. In order to switch from low to high power, you must rotate the revolving nosepiece. The total magnification of a microscope is determined by adding the ocular lens power to the objective lens power.

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