Although we use pre-cast gels in this lab it is common practice to make your own polyacrylamide gels in a laboratory. How much bis-acrylamide (in grams) should you use to make 5 mL of a 5% bis-acrylamide solution ? We tyrically stere rupning buffor (used in SDS RA CELIN HOY

Macroscale and Microscale Organic Experiments
7th Edition
ISBN:9781305577190
Author:Kenneth L. Williamson, Katherine M. Masters
Publisher:Kenneth L. Williamson, Katherine M. Masters
Chapter4: Recrystallization
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2. Although we use pre-cast gels in this lab it is common practice to make your own
polyacrylamide gels in a laboratory. How much bis-acrylamide (in grams) should
you use to make 5 mL of a 5% bis-acrylamide solution?
3. We typically store running buffer (used in SDS-PAGE) in a 10X concentrated
form. You are given 100 mL of a 10X running buffer stock solution and need to
make it all into a 1X running buffer solution. How much water would you add to
the stock solution?
Transcribed Image Text:2. Although we use pre-cast gels in this lab it is common practice to make your own polyacrylamide gels in a laboratory. How much bis-acrylamide (in grams) should you use to make 5 mL of a 5% bis-acrylamide solution? 3. We typically store running buffer (used in SDS-PAGE) in a 10X concentrated form. You are given 100 mL of a 10X running buffer stock solution and need to make it all into a 1X running buffer solution. How much water would you add to the stock solution?
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