An open tubular column with a diameter of 205 um and a stationary phase thickness on the inner wall of 0.51 um passes unretained solute through in 55 s. A particular solute has a retention time of 421 s. 1). What is the distribution constant for this solute? KD= 2). What fraction of time does this solute spend in the stationary phase? tS/ttotal =

An open tubular column with a diameter of 205 um and a stationary phase thickness on the inner wall of 0.51 um passes unretained solute through in 55 s. A particular solute has a retention time of 421 s. 1). What is the distribution constant for this solute? KD= 2). What fraction of time does this solute spend in the stationary phase? tS/ttotal =

Chemistry

9th Edition

ISBN:9781133611097

Author:Steven S. Zumdahl

Publisher:Steven S. Zumdahl

Chapter11: Properties Of Solutions

Section: Chapter Questions

Problem 109AE: Patients undergoing an upper gastrointestinal tract laboratory test are typically given an X-ray...

See similar textbooks

Similar questions

Patients undergoing an upper gastrointestinal tract laboratory test are typically given an X-ray contrast agent that aids with the radiologic imaging of the anatomy. One such contrast agent is sodium diatrizoate, a nonvolatile water-soluble compound. A 0.378-m solution is prepared by dissolving 38.4 g sodium diatrizoate (NaDTZ) in.l.60 102 mL water at 3 1.2C (the density of water at 31.2C is 0.995 g/cm3). What is the molar mass of sodium diatrizoate? What is the vapor pressure of this solution if the vapor pressure of pure water at 31.2C is 34.1 torr?
An open tubular column with a diameter of 215 um and a stationary phase thickness on the inner wall of 0.51 um passes unretained solute through in 79 s. A particular solute has a retetion time of 467 s. What is the distribution constant for this solute? Kp = What fraction of time does this solute spend in the stationary phase? ts !! ttotal
The osmotic pressures of solutions of an enzyme, denoted B, in water at 298 K are given below. Determine the molar mass of the enzyme. Cmass, /g dm-) 1.00 2.00 4.00 7.00 9.00 II/Pa 27 70 197 500 785 Slope: BRT Slope: BRTIM Intercept: RT Intercept: RTIM (a) Molar concentration, c, (b) Mass concentration, cmass,B Fig. 6 The plot and extrapolation made to analyse the results of an osmometry experiment (a) using the molar concentra- tion and (b) using the mass concentration. IVc, C mass,B
GRAVIMETRIC ANALYSIS The addition of dimethylglyoxime, H2C4H602N2, to a solution containing nickel(Il) ion gives rise to a precipitate: Nickel dimethylglyoxime is a bulky precipitate that is inconvenient to manipulate in amounts greater than 135 mg. The amount of nickel in a type of permanent-magnet alloy ranges between 22% and 38%. Calculate the sample size that should not be exceeded when analyzing these alloys for a nickel.
1. Part A Solution temperature = 24 °C Conductivity of instrument grade water = 1.043 uScm! Conductivity of deionised water = 1.74 µScm! Conductivity of tap water = 330 μScml On the basis of data above comment on conditions of the different water grades. 2. The experimental data for electrolytes in Part B are given below: Electrolyte c (M) K (ms.cm') HC1 0.01 5.06 KCI 0.01 1.415 NaCl 0.01 1.236 CaCl2 0.01 2.30 КОН 0.01 2.29 CH3OH 0.01 0.003 K2SO4 0.01 2.65 Calculate molar conductivity A of each electrolyte solution in Sm/mol above. Correct for the conductivity of deionized water.
C. Electrolytes in Body Fluids 1. Type of IV Solution 5% Isolute P (mEa/L) Plasnalyte (mEg/L) Lactated Ringer's (mEg/L) Nal+ = 130 Klt = 4 Ca2+ = 3 Nal+ = 25 K+ = 20 Nal+ = 140 Kl+ = 5 2. Cations %3D %3D Mg+ = 3 Mg2+ = 3 CI- = 22 HPO- =3 Acl- = 23 CI- = 98 Glu'-= 23 Acl- = 27 3. Anions CI'- = 109 %3D HPO = 28 %3D %3D 4. Total Charge of Cations (+) 137 137 5. Total Charge of Anions (-) 6. Sum of the Charges Copyright 2014 Pearson Education, Inc.
12 g of unknown organic sample was dissolve in 706 mL of Dicloromethane (DCM). The boiling point of benzene was increased by 2.72oC. Determine the molecular weight of the unknown sample? Kb of DCM = 2.42oC/m Bb of benzene = 39.6 oC density of benzene = 1.33 g/mL at 25 °C Round your answer to the nearest whole number, no units required.
4.columns with one million theoretical plates operating by slip flow. The figure shows the gradient separation of two forms of fluorescently labeled bovine serum albumin separated on a)50 x 2.1 mm UHPLC column of 1.7 µm C4 silica particles and b)10 mm x 75µm colloidal crystal column of 470 nm C4 silica nanoparticles. What are the main differences in the chromatogram? What is the reason for the difference? Why does the colloidal crystal column have such a high number of theoretical plates (N)? How is the problem of high pressures with very small particles overcome?
What is the minimum distribution constant that permits removal of 99% of a solute from 50.0 mL of water with two 25.0-mL extractions with toluene? five 10.0-mL extractions with toluene?
In 6.75g of distillate >130C. After performing all necessary GC calculations, it is determined that this sample is 75.9% isopentyl acetate. What qas the actual mass(g) of isopentyl acetate that was actually in this sample? Answer to 2 decimal places
What mass of MgCl₂ 6H₂O is required to make a 0.200 dm³ stock solution containing 25.0 ppm Mg(II)? What volumes of this stock solution are required to make 10.0 cm³ standard solutions containing 5.00, 1.00 and 0.10 ppm Mg(II)?
A 18 g of unknown organic sample was dissolve in 756 mL of benzene. The boiling point of benzene was increased by 3.36oC. As the first step of analysis, determine the moecular weight of the unknow sample? Kb of benzene= 2.64oC/m Bb of benzene = 80.09 oC density of benzene = 0.874 g/mL at 25 °C Answer in whole number, no units required.