An unknown amino acid labeled BAYOKEM has an IpH value equal to 5.97. 1. If its pka2 is equal to 9.63, calculate the pKa1 of BAYOKEM. Show complete solutions. 2. Based on the table below, what is the probable identity of BAYOKEM? AMINO ACID pka1 pka2 Proline 1.99 10.96 Tryptophan 2.38 9.39 Valine 2.32 9.62 Glutamine 2.17 9.13 Asparagine 2.02 8.80
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- A tetrapeptide, glutamate-glycine-alanine-lysine, is prepared at at concentration of 1 mM (0.001 M) and is measured in the standard setup (pathlength of 1 cm). What is the approximate absorbance of this peptide at 280 nm? Hint: if the peptide contained a single tryptophan, the answer would be about 10. 10 280 1 0Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pl. The solution is then titrated with 0.2M HCI to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCl needed to reach each relevant pKa value and equivalence point(s). Relevant pka values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.
- Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL XCimetidine is used in the treatment and management of gastric ulcers and gastroesophageal reflux disease. In the case of an active ulcer, cimetidine can be administered continuous by IV infusion at a dose rate of 37.5 mg/hr. If the concentration of the cimetidine solution is 6 mg/ml, then how many milliliters must be available for a 4-hr infusion?Misoprostol is used to prevent NSAID-induced gastric ulcers. A daily dose consists of two tablets administered four times each day. What is the size of one day’s dose if each tablet contains 100 mcg of misoprostol?The fosfomycin disc (200/50) also contains glucose-6-phosphate (G-6-P). Which ONE of the following best describes why this is the case? Select one: A. G-6-P increases the solubility of fosfomycin B. G-6-P is used to promote active transport of fosfomycin into the bacterial cell C. G-6-P is used in the formulation to correct glucose deficiencies promoted by fosfomycin D. G-6-P stabilises fosfomycin from spontaneous hydrolysis in aqueous solution E. G-6-P is an inhibitor of FosA that inactivates fosfomycin
- The following laboratory test results were obtained in a patient with severe jaundice, right upper quadrant abdominal pain, fever, and chills. Serum alkaline phosphatase Four times normalSerum cholesterol IncreasedAST (SGOT) Normal or slightly increased5’-Nucleotidase IncreasedTotal serum bilirubin 25 mg/dLConjugated bilirubin 19 mg/dLProthrombin time Prolonged but improves with a vitamin K injection A) What is the most likely cause of jaundice in the patient? Rationalize your answers.The structure of ephedrine isgiven, The pKa for ephedrine is 9.6. Answer the following questions about aqueous solutions of ephedrine. a. Draw the structure of the principal chemical form of ephedrine that results when it isdissolved in water.b. Draw the structure of the principal chemical form of ephedrine that results when it is dissolved in a buffer at a physiologically relevant pH of 7.4.c. Calculate the pH that results when 0.3 mole of ephedrine is dissolve in 2.0 L of 0.05 M perchloric acid (HClO4).Given the Ramachandran Plot below, identify the protein components that could adopt the phi-psi angle combination indicated by the number 3.
- The isoelectric point, pI, of the protein deoxyribonuclease I is 10.2 , while that of pepsin is 1. What is the net charge of deoxyribonuclease I at pH 7.3 ? What is the net charge of pepsin at pH 3.5 ? The isoelectric point of tryptophan is 5.89 ; phenylalanine , 5.48 . During paper electrophoresis at pH 6.5 , toward which clectrode does tryptophan migrate? During paper electrophoresis at pH 4.5, toward which electrode does phenylalanine migrate? [Diethylaminoethyl cellulose is a positively charged resin used in ion-exchange chromatography with a pKa is about 11. A negatively charged protein with an isoelectric point of 5.0 is applied to the column in a buffer at pH 7 and containing 0.1 M NaCl. Which of the following conditions is most likely to weaken the interaction between the protein and the resin? A Raising the pH to 8 and decreasing the NaCl to 0.05 M B Raising the pH to 8 and increasing the NaCl to 0.2 M C Lowering the pH to 6 and decreasing the NaCl to 0.05 M D Lowering the pH to 6 and increasing the NaCl to 0.2 MThe following table shows the molecular masses (Mr) and isoelectric points (pI) of five proteins: Protein Mr pI Chicken egg white lysozyme 14,000 11.0 Carbonic anhydrase 30,000 5.4 Ovalbumin 45,000 4.5 Phosphorylase b 97,000 6.7 Lactate dehydrogenase 140,000 7.12 (a) A solution containing these five proteins was adjusted to pH 7.0 and then applied to a SIZE-EXCLUSION COLUMN. Assume that these five protein molecules are spherical in shape. Which protein is likely to be eluted LAST from the column? Explain briefly. (b) In another experiment, this protein mixture was first adjusted to pH 6.7 before applying to a CATION-EXCHANGE COLUMN. (i)What is the net charge on phosphorylase b? (ii) Which of these five proteins will bind to the CATION-EXCHANGER? Explain briefly.v