Are all the proteins separated properly using SDS-PAGE? Why or why not?
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Are all the proteins separated properly using SDS-PAGE? Why or why not?
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- Are all the proteins separated properly using two-dimensional (2D) gel electrophoresis? Why or why not?When the gel is removed from the chamber in SDS-PAGE, only the “pre-stained” protein standard is visible. There are no other proteins visible on the rest of the gel. Did something go wrong here? Why can’t you see the other proteins?What is the function of the stacking gel in SDS-PAGE?
- can SDS-PAGE be used to determine the mass of a multimeric protein?What types of homogenization techniques are available for solubilizing a protein?Given the following Wild Type and Mutated DNA sequences: 1.) Identify where the base pair change occurs (what letters changed?) 2.) For BOTH sequences, write the mRNA strands, define the codon regions (with spaces), and amino acid sequences. 3.) Describe what kind of mutation has occurred (missense, nonsense, or silent), and what effect this may have on the protein. Wild Type DNA Sequence: 3' - CCTCGTTATGTG - 5' Mutated DNA Sequence: 3' - CCTCGTTATTTG - 5'
- With this DNA sequenec: - 5'-GCAATGGAGAGAATCTGCGCG-3'- - 3'-CGTTACCTCTGTTAGACGCGC-5' - -Identify the sequencce of RNA in which the protein product will be detrnemined. -What will be the protein product sqeuence? -What will be the pl of the protein product?Given the following Wild Type and Mutated DNA sequences: 1.) Identify where the base pair change occurs ( what letter changed?) 2.) For BOTH sequences, write the mRNA strands, define the codon regions and amino acid sequences. 3.) Describe what kind of mutation has occurred (missense, nonsense, or silent), and what effect this may have on the protein. Wild Type DNA Sequence: 3' - AGGCTCGCCTGT - 5' Mutated DNA Sequence: 3' - AGTCTCGCCTGT - 5'What is single-cell protein? Explain the reasons for the removal or reduction of nucleic acid in Single Cell Protein.
- Describe the common strategy (steps) for protein sequencing, starting with a biological sample containing many cell and biochemical substances. How prevalent are disulfide links in proteins? Why do the disulfide links need to be broken prior to sequencing? How can they be chemically broken?how does polyacrylamide gel electrophoresis with SDS separate proteins ?Which well(A through E) of this agarose gel contains the smallest DNA size? Please look at the pic.