Can you classify household disinfectants as sterilants? What organisms (if any) survive after the use of common household disinfectants that would not survive the use of sterilization/aseptic technique? List a minimum of two organisms that would survive.
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- For each item on the following list, propose a reasonable method ofsterilization. You cannot use the same method more than three timesfor the whole list. The method must sterilize, not just disinfect; and themethod must not destroy the item or render it useless unless there is noother choice. After considering a workable method, think of a methodthat would not work. Note: Where an object containing something isgiven, you must sterilize everything (for example, both the jar and thepetroleum jelly [Vaseline] in it). Some examples of sterilizing methodsare autoclave, ethylene oxide gas, dry oven, and ionizing radiation.room airseruma pot of soilplastic Petri dishesheat-sensitive drugscloth dressingsleather shoes from a thrift shopa cheese sandwicha flask of nutrient agaran entire room (walls, floor,etc.) rubber glovesdisposable syringescarcasses of cows with “madcow” diseaseinside of a refrigeratorwinea jar of Vaselinefruit in plastic bagsmilkorchid seedsmetal instrumentsmail contaminated…Which physical or chemical agent would be the best choice for sterilizing the following items: glass pipettes, tryptic soy broth tubes, nutrient agar, antibiotic solution, interior of a biological safety cabinet, wrapped package of plastic Petri plates? Explain your choices.In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropper
- List two methods of high level disinfection.You are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. 6) What would be the last reagent you use to identify the bacteria? 7) What type of visual aid you need to help you see the bacterial samples clearly? 8) After the use of what reagent, you will be able to decide if you have a Gram positive or Gram negative bacteria? **Please give the answer**From figure shows triplesugar iron agar. What biochemical characteristics does this figureillustrate? How could this medium be used to begin the identificationof the isolated bacteria in this chapter’s Case Study?
- Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:What are the approaches for sterilization during the production of sterile products? Please answer at your own wordsCONTRAST the following pairs of terms: Sterilize and Disinfect Sanitization and Antisepsis Bactericide and Bacteriostatic
- Explain why it is important to use only a small amount of bacteria when preparing a smearMake a list of the different types of sterilization, including the method, the principle, the materials that can be sterilized, the required temperature and time, and the required temperature and time. Method Principle Apparatus Temp/Time MaterialsDefine the following terms; Sterilization, and Aseptic Technique. What are the differences between the two concepts?