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1. Calculate the size of the resulting fragments as they will occur after digestion and write the sizes on the maps below. Note that linear DNA has a total size of 48 502 bp (see figure 3A).

1. Calculate the size of the resulting fragments as they will occur after digestion and write the sizes on the maps below. Note that linear DNA has a total size of 48 502 bp (see figure 3A).

Biology 2e
Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter14: Dna Structure And Function
Section: Chapter Questions
Problem 18RQ: During proofreading, which of the following enzymes reads the DNA? primase topoisomerase DNA pol...
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18:20 O O OO0 • 4G+ * ll 7% Restriction enzyme tutorial 2021_1_ - Saved 1. Calculate the size of the resulting fragments as they will occur after digestion and write the sizes on the maps below. Note that linear DNA has a total size of 48 502 bp (see figure 3A). %3D B BamHI 5305 22,346 27,972 34499 41,732 op) ECORI, 21,226 26,104 31,747 39,168 44,972 (bp) D Hindll 23,130 27,479 36,895 37,584 44141 25,157 (bp) 37,459 2. How many fragments would you expect to see for each of the maps indicated in question 1 above? Мар В: Мар С: Мар D: 3. Use the sequence indicated in figure 1 (also refer to figure 2 and 3) and indicate how many times does the sequence GAATTC occur in the A DNA sequence? What about AAGCTT and GGATCC? GGATC: GAATTC: АAGCTT: B IU e A = E
Transcribed Image Text:18:20 O O OO0 • 4G+ * ll 7% Restriction enzyme tutorial 2021_1_ - Saved 1. Calculate the size of the resulting fragments as they will occur after digestion and write the sizes on the maps below. Note that linear DNA has a total size of 48 502 bp (see figure 3A). %3D B BamHI 5305 22,346 27,972 34499 41,732 op) ECORI, 21,226 26,104 31,747 39,168 44,972 (bp) D Hindll 23,130 27,479 36,895 37,584 44141 25,157 (bp) 37,459 2. How many fragments would you expect to see for each of the maps indicated in question 1 above? Мар В: Мар С: Мар D: 3. Use the sequence indicated in figure 1 (also refer to figure 2 and 3) and indicate how many times does the sequence GAATTC occur in the A DNA sequence? What about AAGCTT and GGATCC? GGATC: GAATTC: АAGCTT: B IU e A = E
18:20 O O OO0 • 4G+ * ll 7% Restriction enzyme tutorial 2021_1_ - Saved 4. Draw these fragments as they would migrate on the electrophoresis gel. Write the size of the fragments on the square when you draw them. 5. According to your answers provided at question 2 there should be a certain amount of Ladder BamHI EcoRI Hindll bp 50,000 24,000 20,000 16,000 10,000 9,000 8,000 7,000 6,000 5,000 4,000 3,000 2,000 1,000 bands visible on your gel. Suppose you did this practical and you observed less bands on your gel, give possible explanations for this. B IU e DNA migration ||
Transcribed Image Text:18:20 O O OO0 • 4G+ * ll 7% Restriction enzyme tutorial 2021_1_ - Saved 4. Draw these fragments as they would migrate on the electrophoresis gel. Write the size of the fragments on the square when you draw them. 5. According to your answers provided at question 2 there should be a certain amount of Ladder BamHI EcoRI Hindll bp 50,000 24,000 20,000 16,000 10,000 9,000 8,000 7,000 6,000 5,000 4,000 3,000 2,000 1,000 bands visible on your gel. Suppose you did this practical and you observed less bands on your gel, give possible explanations for this. B IU e DNA migration ||
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Can you please answer question 5.

According to your answers provide at question 2 there should be a certain number of bands visible on your gel. Suppo. you did this practical and you observed less bands on your gel, give possit I explanations for this.

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