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Chromatin Immunoprecipitation (ChIP) experiments enable researchers to measure the levels of transcription factors, coactivators/corepressors and chromatin remodeling complexes on a specific gene in cells. In the ChIP experiment below, the recruitment of the transcription factor NfkB, Histone Deacetylase Complex (HDAC3) and p300/CBP complex to the Interleukin 12 (IL12) gene at various times (0, 30, 60 and 120 min) after treating cells with LPS are measured. Does LPS stimulate or inhibit transcription of the IL12 gene based on the recruitment of factors to the IL12 gene.

Chromatin Immunoprecipitation (ChIP) experiments enable researchers to measure the levels of transcription factors, coactivators/corepressors and chromatin remodeling complexes on a specific gene in cells. In the ChIP experiment below, the recruitment of the transcription factor NfkB, Histone Deacetylase Complex (HDAC3) and p300/CBP complex to the Interleukin 12 (IL12) gene at various times (0, 30, 60 and 120 min) after treating cells with LPS are measured. Does LPS stimulate or inhibit transcription of the IL12 gene based on the recruitment of factors to the IL12 gene.

Medical Terminology for Health Professions, Spiral bound Version (MindTap Course List)
Medical Terminology for Health Professions, Spiral bound Version (MindTap Course List)
8th Edition
ISBN:9781305634350
Author:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder
Publisher:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder
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Chromatin Immunoprecipitation (ChIP) experiments enable researchers to measure the levels of transcription factors, coactivators/corepressors and chromatin remodeling complexes on a specific gene in cells. In the ChIP experiment below, the recruitment of the transcription factor NfkB, Histone Deacetylase Complex (HDAC3) and p300/CBP complex to the Interleukin 12 (IL12) gene at various times (0, 30, 60 and 120 min) after treating cells with LPS are measured. Does LPS stimulate or inhibit transcription of the IL12 gene based on the recruitment of factors to the IL12 gene. Justify your answer by explaining the state of the gene before LPS and after LPS treatment (limit 5-6 sentences).

 

IP NFkB HDAC3 p300 IgG Input WT 0 30 60 120
Transcribed Image Text:IP NFkB HDAC3 p300 IgG Input WT 0 30 60 120
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