Chromatography A student in lab used chromatography to separate the pigments present in an extract of spinach leaves. Using your knowledge of chromatography, calculate the retention factor for each pigment present on the chromatogram. Distance (cm) 9.5 Pigment Rf value .916 8.7 Carotene Xanthophyll .431 4.1 2.2 Chlorophyll a .231 Chlorophyll b .189 1.8 0.0 Leaf stain 0.0

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter34: Miscellaneous Separation Methods
Section: Chapter Questions
Problem 34.8QAP
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See attached. Ignore my RF factors. I don’t know if they are right.
Chromatography
A student in lab used chromatography to separate the pigments present in an extract of
spinach leaves. Using your knowledge of chromatography, calculate the retention factor
for each pigment present on the chromatogram.
Distance (cm)
9.5
Pigment
Rf value
.916
8.7
Carotene
Xanthophyll
.431
4.1
2.2
Chlorophyll a
.231
Chlorophyll b
.189
1.8
0.0
Leaf stain
0.0
Transcribed Image Text:Chromatography A student in lab used chromatography to separate the pigments present in an extract of spinach leaves. Using your knowledge of chromatography, calculate the retention factor for each pigment present on the chromatogram. Distance (cm) 9.5 Pigment Rf value .916 8.7 Carotene Xanthophyll .431 4.1 2.2 Chlorophyll a .231 Chlorophyll b .189 1.8 0.0 Leaf stain 0.0
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