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- Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct) a acid alcohol b crystal violet c carbolfuschin d malachite green e safranin f iodine g alcohol h methylene blueWhat staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.Is is best to observe slides of bacteria stained with the acid fast stain under high dry magnification. True or false
- Describe one error in the staining procedure that would result in a Gram-positive bacterium appearing pink.Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a day
- The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in performing the acid-fast stain technique because of its thin cell wall. However, if this organism is used for Gram-stain, it can also take the Gram stain reaction. Why is this so?About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this type of coloring?Gram-positive (+) bacteria with a thin peptidoglycan layer traps the crystal violet stain. Select one: True False
- Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain affect the staining of bacteria? Can dyes other than methylene blue be used for direct staining? Briefly explain.Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple stain, and differential stain. please provide minimum of 400 wordsWhat stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.