Q: A food sample with an unknown number of contaminating bacteria was serially diluted to 10-6. A…
A: Serial dilution of a food sample containing unknown quantity of contaminating bacteria was performed…
Q: What type of medium is used to maintain and preserve specimens before clinical analysis? a.…
A: Microorganisms are small, unicellular, and microscopic organisms. Any organism capable of causing…
Q: You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria…
A: The bacteria can grow only in the favourable conditions that is when there is optimum amount of pH,…
Q: In spread plate method, colonies form within the agar and agar surface. In pour plate method,…
A: Introduction Agar media is essential for the study of microorganisms and molecular biology, It is a…
Q: Why is the level of contamination measured as number of colonies rather than size of colonies?
A: Microbiological contamination refers to the presence of unwanted or accidental introduction of…
Q: Mention any three (3) fluids used in cleaning lenses in the microbiological lab
A: Microscopes have to be well maintained in order to have a longer life for the instrument. Objective,…
Q: When can gelatin be used in culture media preparation? [Answer in not more than 2 sentences
A: GELATIN: Gelatin, often known as gelatine, is a transparent, colourless, and flavourless culinary…
Q: Indicate the correct way of inoculating the following media. Plate Media For sensitivity testing…
A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…
Q: Physical methods of sterilization include :
A: disclaimer: since you have posted multiple questions we have answered the first question for you. if…
Q: procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar…
A: Aseptic Technique describe some of the ways that a laboratory can deal with the constant threat of…
Q: Give the uses/functions and images of each apparatuses Basic Laboratory Equipment Uses/Functions…
A: Antibiotic discs are used in microbiological studies to observe the effects of various antibiotics…
Q: The mechanical removal of microbes from a limited area of living tissue is referred to as -- a.…
A: Control of microbial propagation is very significant in medical, pharmaceutical, food industries,…
Q: ure bacterial culture was diluted by adding a 0.2 mL aliquot to 0.9mL water. Then 0.1 mL of this…
A: In microbiology, pure culture, a laboratory culture which contains one organism species. A pure crop…
Q: Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions…
A: Note: I can't provide you with pictures. But i will explain you functions of these apparatus.…
Q: How can I tell if there is contamination?
A: RFLP is a type of DNA polymorphism that has DNA sequence variations that can be recognized by…
Q: Transparent double-sided dishes used for growing microbes are most commonly called A) Petri dishes.…
A: The micro organisms are grown in specific containments for identification of the characteristic…
Q: Can culture media be sterilized in the hot air sterilizer? Why or why not? (Answer in not more than…
A: Sterilization is defined as a process by which an agent is used to destroy or remove the…
Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…
A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…
Q: You were given a mixed nutrient agar broth culture of bacteria 1a. How will you determine the…
A: A mixed bacterial culture consists of more than one bacterial cell types growing together. On the…
Q: 2. Mechanical disinfection is typically more effective than manual techniques.
A: Disinfection is the removal, deactivation, and killing of microorganisms. Deactivation and…
Q: Could you spread plate in the usual Petri dish a 10mL inoculum?
A: The spread plate method is a technique to plate a liquid sample containing bacteria so that bacteria…
Q: Oven sterilization of aqueous culture medium. Effect: Rationale:
A: Oven sterilization utilizes dry heat of about 160 or 170 degrees celsius for one hour or 30 minutes,…
Q: Microscopic count is used to estimate microbial load of a sample by counting colonies present on the…
A:
Q: I need help with question number 49
A: Microbial control indicates the prevention or inhibition of microbial growth. It involves physical…
Q: Aseptic technique refers to A) the microbial inoculum placed into a test tube or onto a Petri…
A: Following aseptic techniques is an important part while performing an experiment. Aseptic technique…
Q: C MSA (Mannitol Salt Agar) Match the description to the correct Bacterium A= growth, agar is yellow…
A: Microbiology is the branch of science that deals with the study of microorganisms that are too small…
Q: differential plate enrichment plate selective plate streak plate pour plate
A: Differential plate: Differential media used to distinguish between two related microbes. It used to…
Q: Name six basic nutritional requirements supplied in all culture media.
A: The culture media refers to the gel or liquid prepared for the growth of microorganisms. The culture…
Q: The culture primarily contains nutrients such as sugars and a gel (agar). Why don’t viruses grow in…
A: Culture media are an integral part of the microbiology lab. There are various kinds of media used in…
Q: 1-The method of sterilizations we used in the tissue culture lab a. Fumigation b. Filtration C.…
A: The removal of any microorganism or viable spores or bacteria or viruses from the surface. The…
Q: The main of objective of using 13-Line streaking method is to get:
A: Answer: 13-Line streaking method: It is the technique of obtaining pure bacterial culture by…
Q: Differentiate sterilization from disinfection.
A: Methods to remove unwanted microbes from the surfaces, clothes, and bodies are important.…
Q: Which of the following medium would be most appropriate for the bacterial cultures from stomach? a.…
A: Microorganism culture out of their natural environment in pure form in the laboratory has been…
Q: ow can one be sure that the culture media are sterile or free from contamination
A: Sterility, often known as sterility, is the state of being free of all contaminants and…
Q: FILTERING IS FILTERING 1. physical method of disinfection 2. the physical method of water…
A: Q. FILTERING IS FILTERING 1. physical method of disinfection 2. the physical method of water…
Q: What will pose an ethical issue in these procedures are performed - mutilation, sterilization?
A: Sterilization is a procedure which is practiced to limit the family size in which removal or simply…
Q: 1. Differentiate the types of culture media based on its physical state and uses.
A: MICROBES They are unicellular organisms that cannot be seen with the naked eye.This is why they are…
Q: Explain shortly the concept of differential centrifugation and how you would limit pellet…
A: Centrifugation is a mechanical technique. It helps to separate mixtures based on their size, shape,…
Q: What things can be done during sample collection of a sick patient to help eliminate the culture of…
A: Normal flora is the organisms that are present in the body for normal functioning. The normal flora…
Q: Which of the following is used in the sterilization of the medium? a) benmarge b) swab c) autoclave…
A: Sterilization is the process of making something (instrument, swab, room etc. ) free of microbes…
Q: what is your initial assumption of the organism causing this disease
A: Bacteria are the prokaryotic microorganism and based on the structural cell walls, Gram stain…
Q: how could you determine if there is any contamination on your agar?
A: Agar consists of two polysaccharides, agarose and agaropectin, but agarose making up about 70% of…
Q: Which of the following is not one of the six “I’s”? a. inspection d. incubation b. identification e.…
A: Microbes: Microbes are not visible to the naked eye and they grow in complex associations.…
Q: Give the use and advantages of the following cultures for the examination of colony characteristics:…
A: Culture media: a. Culture media provides an environment that supplies all essential nutrition for…
Q: Identification of an organism does not require a pure culture because Bergey’s Manual will sort out…
A: Taxonomy is the branch of biology, which deals with the science of naming, defining and classifying…
Q: explain why you would expect to see similarities in the types of colonies on the fomite plates and…
A: Organisms that are too small to be seen by the naked eye are microorganisms. These organisms can be…
Q: Below is a diagram of the serial dilution of a culture with 1 x 10° cells. Fill out the missing…
A: Colony-forming unit / mL= Number of colonies * Dilution factor * 1/volume plated in mL CFU/mL = 1 *…
Q: Which of the following methods is most sensitive for identifyingdifferent strains of a microbe?a.…
A: Microorganisms or microbes are small living organisms that can be observed with the aid of…
Q: bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows:…
A: CFUs are a measure of the number of progenitors present in a given population of cells; if an…
22. A pure culture refers to a culture that contains multiple types of organisms.
a) True
b) False
Step by step
Solved in 2 steps
- 5) A pure culture A) is sterile . B) is a population of identical cells. C) is made of a clearly defined chemical medium. D) contains one microbial cell.1. The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization? 3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distilled5) Aseptic technique refers to A) the microbial inoculum placed into a test tube or onto a Petri plate. B) a series of practices to avoid contamination. C) the autoclave and other sterilizing procedures. D) cleanliness in the laboratory.
- 1. Sally Monella found 344 bacterial colonies on one of her plates. She had prepared this plate after a serial dilution of a culture of Yersinia pestis. She had pipetted 1 ml sample of diluted Yersinia pestis from a tube with the overall dilution factor of 106 into a plate and covered it with agar. She used this plate to calculate the concentration in cells/ml of a bacterial culture. a) How many organisms were in the original culture? b) Were her results valid? Why or why not?The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization?3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distilleWhat is the difference between a mixed culture and a contaminated culture if they both contain more than one type of organism ?
- Transparent double-sided dishes used for growing microbes are most commonly called A) Petri dishes. B) baker dishes. C) sterilization plates. D) culture medium plates.How do you observe growth in liquid microbiological media? a) the medium is cloudy or turbid b) colonies appear on the surface of the medium c) the medium becomes solid d) colonies appear distributes throughout the medium15. Colony formation can be observed in liquid media. a) True b) False
- 1. A plate with a final dilution factor of 107 produced 210 colonies. a) What was the original concentration in the sample? b) If you were to dilute the original sample with a dilution factor of 108, how many colonies would you count on the plate? c) If you were to dilute the original sample with a dilution factor of 106, how many colonies would you count on the plate? 2. Sally Monella found 344 bacterial colonies on one of her plates. She had prepared this plate after a serial dilution of a culture of Yersinia pestis. She had pipetted 1 ml sample of diluted Yersinia pestis from a tube with the overall dilution factor of 106 into a plate and covered it with agar. She used this plate to calculate the concentration in cells/ml of a bacterial culture. a) How many organisms were in the original culture? b) Were her results valid? Why or why not?a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteriaYou mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do not know if you grew staph epidermis or E. coli. You found that the organism growing on the mannitol salt agar remained red after incubation. It is most likely that the organism is E.coli. a) True b) False