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Describe how you would genetically design a superbug resistant to β-lactams, methicillin, streptomycin, daptomycin, and trimethoprim.
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- Describe how you would genetically design a superbugresistant to b-lactams, methicillin, streptomycin, daptomycin,and trimethoprimA series of auxotrophic mutants were isolated in Neurospora. Examination of fungi containing these mutations revealed that they grew on minimal medium to which various compounds (A, B, C, D) were added; growth responses to each of the four compounds are presented in the following table. Give the order of compounds A, B, C, and D in a biochemical pathway. Outline a biochemical pathway that includes these four compounds and indicate which step in the pathway is affected by each of the mutations. Compound Mutation number А в С A D 134 276 987 773 772 146 333 123 + + I +Various antimicrobial drugs to treat microbial infection have diverse mechanism of action. Consider the following antimicrobial drugs: A. Seconeolitsine, known as DNA topoisomerase I inhibitor in bacteria. (i) Explain briefly how inhibiting DNA topoisomerase I is a good mechanism of action for an antibiotic, include possible molecular machineries being targeted. (ii) What would be an appropriate response if seconeolitsine works well by stating the state of supercoiling in bacteria. (iii) To prove your answer (ii), you test the condition of bacterial DNA by running gel electrophoresis, one has been treated with seconeolitsine (+ sample) and the other one is not (- sample). Explain the position of each + sample and – sample band on the gel in reference to the point of origin (where you load your samples) or how far each DNA sample travel across agarose gel. (iv) Explain why you would expect answer (iii) for each + sample and – sample. B.…
- What is the name of the fungal toxin that inhibits RNA polymerase Il at 1 microgram/mL concentration? O Flavopiridol a-Amanitin O Triptolide DRB (5,6-Dichloro-1-ß-D- ribofuranosylbenzimidazole)Illustrate how latex agglutination works to bind to ASO and form agglutinatesDescribe an experiment in which you demonstrate that the pUL44 protein of Cytomegalovirus (CMV) localizes with phospho-H2AX (yH2AX). What reagent can be used to denote the cellular location of H2AX? Be sure to carefully describe your controls, as well as the expected results. What other technique can be used to validate the findings in this experiment? Explain.
- write the detail explation of mode of action of vincristine in detail . write the detail explation of mode of action of paclitaxel in detail ( these are microtubule damaging agent )What is the genus and species of your bacteria? And What led you to confirm this result and why? Please discuss. ☆My bacteria is Alcaligenes faecalisYou have isolated a beta-lactamase producing Staphylococcus aureus (not a MRSA strain) from an infected surgical site on your patient. If for genetic reasons, your patient is allergic to all antibiotics except beta-lactam antibiotics such as ampicillin ( they can only take Beta-lactam antibiotics such as ampicillin), which strategy below could you use to treat this Staphylococcus aureus infection in your patient? Note different answers compared to previous question. give the patient erythromycin can use a beta-lactamse resistant beta-lactam such as methicillin or oxacillin O give the patient penicillin give the patient an azole drug
- What does the two component signal transduction system allow cells to do? What are the two components and what do they do? How was this type of system used in Streptococcus pneumoniae transformation?Explain why penicillin therapy may result in the formation of Lforms but tetracycline therapy would not.Ritonavir inhibits the action of HIV protease. What kind of inhibition is imposed on HIV protease by ritonavir?