Draw the gel electrophoresis results for the following 16 kb plasmid when the plasmid is cut with restriction enzyme A, with restriction enzyme B and with both A and B restriction enzymes. The A enzyme cuts at positions 0 and 9.2 kb and the sites are shown as white. (0 is at the top of the circle.) The B enzyme cuts at positions 3.8, 7 and 13.2 kb and the sites are shown as gray. Show the electrical polarity of the gel, the bands, and their sizes in your drawing. Make sizes consistent between gel lanes. Explain why the concept of charge density is important for gel electrophoresis.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
restriction enzyme A, with restriction enzyme B and with both A and B restriction enzymes. The A
enzyme cuts at positions 0 and 9.2 kb and the sites are shown as white. (0 is at the top of the circle.) The
B enzyme cuts at positions 3.8, 7 and 13.2 kb and the sites are shown as gray. Show the electrical
polarity of the gel, the bands, and their sizes in your drawing. Make sizes consistent between gel lanes.
Explain why the concept of charge density is important for gel electrophoresis.
Step by step
Solved in 4 steps with 2 images
