During the chromatography the solvent may be changed from hexanes/ ethyl acetate (8:2) to hexanes/ ethyl acetate (4:6) depending on which ferrocenes are present in the mixture. What is the reason for doing this? Which of these solvent mixtures is more polar? How would you prepare 200 mL of hexanes/ ethyl acetate (8:2)?
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- Please answer all 5 questions (it could be short answers). 5-1. What is the main barrier to separating liquid mixtures of less than 500 mL by distillation? 5-2. A sample mixture of ethyl benzoate (bp 212 Celsius) and dodecane (bp 216.2 Celsius) is injected on two GC columns. Column A has DC710 silicone oil as the stationary phase, and column B uses polyethylene glycol as the stationary phase. Which substance would be certain to elute first from column A and would the same material be expected to elute first from column B? Which column, A or B, would be expected to give the better separation of these two substances? 5-3. Question 5-2 refers to separating a mixture of two high boiling liquids by gas chromatography. These materials have similar boiling points. List several GC variables and conditions that would make it easier to separate thesesubstances by gas chromatography 5-4. Capillary GC columns have better resolution than packed columns even though the enormous surface area…2. You need to make 500mL of 7H9(+) media with 1X ADN and 30ug/ml. of the antibiotic kanamycin. Your stock solutions are 10X ADN and 30mg/mL of kanamycin. The solvent is distilled water. How much of cach component will you need to make the desired final solution?How much drying agent, MgSO4, would be needed for drying of 20mL dichloromethane solution that contains 0.01% water, knowing that each mole of MgSO, can absorb 7 moles of H;0?
- Separate a mixture of Vitamin D and Vitamin B2 (riboflavin) by paper chromatography. The stationary phase is methanol (CH;OH) and the mobile phase is pentane (C;H12). Place the vitamins in order of elution. Explain why using IMF. (Circle polar sites on each molecule and label all 4 molecules as polar or non-polar.) Vitamin Ba 0: H3C NH H3C CH3 CH3 CH3 H3C He H3C HO CH2 Vitamin D He CH2OH HOWhat is the minimum distribution coefficient that permits removal of 98% of a solute from 50.0 mL of water with two 10.0-mL extractions with toluene? (Rounding was done in the calculation. Please select the nearest answer.) O 15 25 O 30 35 O 17 Other:Students were tasked to conduct a recrystallization experiment to test the ability of the newly discovered solvents in their laboratory to purify and separate a mixture containing compounds X and Y. The solubility of the two compounds in g/50 mL of hot and cold solvents are given by the photo.
- 11. How much caffeine can be extracted using 50.0ml of DCM if a tea bag contains 15 grams of tea leaves with 0.9% of caffeine? And has undergone solid-liquid extraction with 100ml of distilled water followed by liquid-liquid extraction using DCM. The solubility of caffeine requires 15 ml of water and 2.0ml of DCM for 40.0mg. a 6.1 x 10^-5 b 6.0 x 10^-5 c 7.0 x 10^-5 d 7.1 x 10^-5What is the minimum distribution constant that permits removal of 99% of a solute from 50.0 mL of water with five 10.0-mL extractions with toluene?6.0 mL of cyclohexanol, 8 mL of 85% phosphoric acid, and 10 drops of sulfuric acid are added to a 50-mL round-bottom flask with boiling chips, and the mixture is distilled into an ice-cooled receiver. The distillate is in the form of a colorless bi-phasic mixture. The cyclohexene upper layer is removed from the water layer, and dried over anhydrous calcium chloride. Isolated is cyclohexene as a clear and colorless foul-smelling liquid. Weighing data is given below. Gross mass: 51.24 g Tare mass: 48.04 g Product mass: 3.2g Question: Calculate the theoretical mass of the cyclohexene product, bearing in mind that the cyclohexanol starting material was measured by volume, not by mass (so you must use the density of cyclohexanol to convert milliliters to grams, before calculating the number of moles). Calculate the percent yield of cyclohexene obtained.
- PLEASE USE ANOTHER SOLUTION. WRITE ALL THE PROCESS. What is the minimum distribution constant that permits removal of 99% of a solute from 50.0 mL of water with five 10.0-mL extractions with toluene?16) Which of the following(s) is/are correct? I- Selectivity factor is the amount of time a solute spends in the stationary phase relative to the time it spends in mobile phase II- The retention time for an analyte is the time interval between its injection onto a column and its appearance at the detector at the other end of the column. III- Distribution constant is the ratio of the total solute concentration in the stationary phase to that in the mobile phase. A) I, II and III B) Only II C) I and III D) II and III E) Only III for GC24g 5 g 2 g 100 g 49." R Lactic acid Salicylic acid Trichloroacetic acid Flexible collodion qs ad Sig: wart remover. Use as directed. (a) Flexible collodion contains 20% w/w camphor and 30% w/w castor oil. How many grams of each would be contained in 30 g of the mixture? (b) The specific gravity of castor oil is 0.955. How many milliliters of the oil is contained in 30 g of the mixture? (c) If the specific gravity of the mixture is 0.781, what are the percent w/v con- centrations of lactic acid, salicylic acid, and trichloroacetic acid in the mixture?